PMID- 34118042
OWN - NLM
STAT- MEDLINE
DCOM- 20210806
LR  - 20210806
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2275
DP  - 2021
TI  - mTRIP, an Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology 
      and Disease at the Single-Cell Resolution.
PG  - 247-263
LID - 10.1007/978-1-0716-1262-0_15 [doi]
AB  - Mitochondrial physiology and metabolism are closely linked to replication and 
      transcription of mitochondrial DNA (mtDNA). However, the characterization of 
      mtDNA processing is poorly defined at the single-cell level. We developed mTRIP 
      (mitochondrial Transcription and Replication Imaging Protocol), an imaging 
      approach based on modified fluorescence in situ hybridization (FISH), which 
      simultaneously reveals mitochondrial structures committed to mtDNA initiation of 
      replication as well as the mitochondrial RNA (mtRNA) content at the single-cell 
      level in human cells. Also specific RNA regions, rather than global RNA, can be 
      tracked with mTRIP. In addition, mTRIP can be coupled to immunofluorescence for 
      in situ protein tracking, or to MitoTracker, thereby allowing for simultaneous 
      labeling of mtDNA, mtRNA, and proteins or mitochondria, respectively. Altogether, 
      qualitative and quantitative alterations of the dynamics of mtDNA processing are 
      detected by mTRIP in human cells undergoing physiological changes, as well as 
      stress and dysfunction. mTRIP helped elucidating mtDNA processing alterations in 
      cancer cells, and has a potential for diagnostic of mitochondrial diseases.
FAU - Chatre, Laurent
AU  - Chatre L
AD  - Institut Pasteur, Stem Cells and Development, Department of Developmental and 
      Stem Cell Biology, CNRS UMR3738 Team "Stability of Nuclear and Mitochondrial 
      DNA", Paris, France.
AD  - Normandie Universite, UNICAEN, CEA, CNRS, ISTCT/CERVOxy group, GIP Cyceron, Caen, 
      France.
FAU - Ricchetti, Miria
AU  - Ricchetti M
AD  - Institut Pasteur, Stem Cells and Development, Department of Developmental and 
      Stem Cell Biology, CNRS UMR3738 Team "Stability of Nuclear and Mitochondrial 
      DNA", Paris, France. miria.ricchetti@pasteur.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (RNA, Mitochondrial)
SB  - IM
MH  - Animals
MH  - DNA, Mitochondrial/*chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mitochondria/*genetics
MH  - Mitochondrial Diseases/*genetics
MH  - RNA, Mitochondrial/chemistry
MH  - Single-Cell Analysis/*methods
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - DNA replication
OT  - FISH
OT  - Imaging
OT  - Mitochondrial DNA
OT  - Mitochondrial disease
OT  - Single-cell
OT  - Transcription
OT  - mTRIP
EDAT- 2021/06/13 06:00
MHDA- 2021/08/07 06:00
CRDT- 2021/06/12 12:12
PHST- 2021/06/12 12:12 [entrez]
PHST- 2021/06/13 06:00 [pubmed]
PHST- 2021/08/07 06:00 [medline]
AID - 10.1007/978-1-0716-1262-0_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2275:247-263. doi: 10.1007/978-1-0716-1262-0_15.