PMID- 34118145
OWN - NLM
STAT- MEDLINE
DCOM- 20210803
LR  - 20210803
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 30
IP  - 7
DP  - 2021 Jul
TI  - Investigating the inflammatory cascade effect of basophil activation in children 
      with allergic rhinitis or asthma, via the IgE-FcepsilonRI-NF-kappaB signaling pathway.
PG  - 673-679
LID - 10.17219/acem/135756 [doi]
AB  - BACKGROUND: Allergic rhinitis (AR) is a type I allergic reaction mediated by 
      serum immunoglobulin E (IgE). OBJECTIVES: This study aimed to investigate the 
      basophil activation rate and serum expression of inflammatory factors in AR 
      patients and rats. The involvement of the IgE-FcepsilonRI-nuclear factor kappa B 
      (NF-kappaB) signaling pathway in the pathogenesis of AR was also explored. MATERIAL 
      AND METHODS: Thirty patients with pediatric AR (or asthma; untreated) were 
      enrolled in our hospital as the observation group. Fifteen healthy children were 
      selected as the control group. The basophil activation and serum levels of 
      interleukin (IL)-4, IL-5, IL-13, IgE, and prostaglandin D2 (PGD2) in 2 groups of 
      children were detected. Forty-eight male Sprague Dawley rats were randomly 
      divided into group A (AR model group), group B (AR model + IgE inhibitor), group 
      C (AR model + omalizumab), and group D (control). The animal model was 
      established by intraperitoneal injection of ovalbumin and nasal mucosa 
      stimulation. The basophil activation rate, serum levels of IL-4, IL-5, IL-13, 
      IgE, and PGD2 were analyzed. RESULTS: In children with AR, the blood samples 
      contained mainly activated basophils, whereas the healthy group showed mainly 
      non-activated basophils. The levels of IL-4, IL-5, IL-13, IgE and PGD2 in the 
      serum of the AR group were higher than those in healthy subjects (p < 0.05). In 
      animal studies, the blood samples collected from group A were mainly activated 
      basophils, while the samples of the other groups were mainly non-activated 
      basophils. The serum contents of IL-4, IL-5, IL-13, IgE, and PGD2 in group A were 
      significantly higher than those in groups B and C (p < 0.05). CONCLUSIONS: 
      Patients with AR showed increased activation of basophils and elevated expression 
      of IL-4, IL-5, IL-13, IgE, and PGD2. The upregulation of FcepsilonRI and NF-kappaB was 
      involved in the pathogenesis of AR.
FAU - Qiao, Yi
AU  - Qiao Y
AD  - Department of Otorhinolaryngology, National Children's Medical Center, Shanghai 
      Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 
      China.
FAU - Chen, Jie
AU  - Chen J
AD  - Department of Otorhinolaryngology, National Children's Medical Center, Shanghai 
      Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 
      China.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - 0 (NF-kappa B)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - *Asthma
MH  - Basophils
MH  - Child
MH  - Humans
MH  - Immunoglobulin E
MH  - Male
MH  - NF-kappa B/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Rhinitis, Allergic
MH  - Signal Transduction
OTO - NOTNLM
OT  - IgE-FcepsilonRI-NF-kappaB
OT  - allergic rhinitis
OT  - basophil
EDAT- 2021/06/13 06:00
MHDA- 2021/08/04 06:00
CRDT- 2021/06/12 12:17
PHST- 2021/06/13 06:00 [pubmed]
PHST- 2021/08/04 06:00 [medline]
PHST- 2021/06/12 12:17 [entrez]
AID - 10.17219/acem/135756 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2021 Jul;30(7):673-679. doi: 10.17219/acem/135756.