PMID- 34118276
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20211130
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 209
DP  - 2021 Aug
TI  - Age-related differences in corneal nerve regeneration after SMILE and the 
      mechanism revealed by metabolomics.
PG  - 108665
LID - S0014-4835(21)00231-1 [pii]
LID - 10.1016/j.exer.2021.108665 [doi]
AB  - PURPOSE: To investigate the effect of age on wound healing after small incision 
      lenticule extraction (SMILE) and the underlying metabolomic mechanisms. METHODS: 
      This prospective study was conducted on 216 patients in four groups: the 18-20 
      (n = 38, Group I), 21-30 (n = 84, Group Ⅱ), 31-40 (n = 58, Group Ⅲ), and 41-50 
      (n = 36, Group IV) age groups. The density of corneal epithelial wing cells, 
      basal cells, corneal stromal cells, endothelial cells and corneal nerves were 
      examined with a laser confocal microscope (HRT III-RCM) before and 1 month, 3 
      month, 6 month and 1 year after SMILE. The central nerve fiber length (CNFL), the 
      central corneal nerve fibre density (CNFD), and the central corneal nerve branch 
      density (CNBD) were analyzed by Nero J. The corneal stroma lenticules were 
      obtained from SMILE to analyze metabolites by high-performance liquid 
      chromatography coupled with quadrupole time-of-flight tandem mass spectrometry 
      (HPLC-QTOF-MS). RESULTS: The density of corneal wing epithelial cells and basal 
      epithelial cells have no significant difference among the four groups. The CNFL 
      was 21.90 +/- 1.68 mm/mm(2) in Group Ⅰ and 21.63 +/- 2.09 mm/mm(2) in Group Ⅱ after 1 
      year of SMILE, which represented a return to the preoperative level, whereas the 
      CNFL of Group Ⅲ (19.40 +/- 0.98 mm/mm(2)) and Group Ⅳ (18.94 +/- 0.72 mm/mm(2)) were 
      lower than that preoperation (P <0.01). CNFL repair had a negative correlation 
      with age after surgery (Pearson's R = -0.572, P <0.01). The CNFD and the CNBD 
      showed the same trend with the CNFL (Pearson's R = -0.602 and -0.531, P <0.05). 
      Through screening the significantly different metabolites between the 18-30 age 
      group (including Group I and Group Ⅱ) and other two groups, 6 common remarkably 
      different metabolites were identified. Meanwhile, 5 unique different metabolites 
      were identified only between the 18-30 age group and the 31-40 age group. Six 
      unique different metabolites were identified only between the 18-30 age group and 
      the 41-50 age group. CONCLUSION: Corneal nerve repair after SMILE was 
      significantly affected by age. The identified age-associated differences in 
      metabolites were mainly related to inflammation, oxidation, nerve protection and 
      regeneration.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Li, Min
AU  - Li M
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, 
      Shanghai, China. Electronic address: rgswan@163.com.
FAU - Liu, Lin
AU  - Liu L
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, 
      Shanghai, China.
FAU - Shi, Yuehui
AU  - Shi Y
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, 
      Shanghai, China.
FAU - Sun, Lina
AU  - Sun L
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, 
      Shanghai, China.
FAU - Ma, Xiaoyun
AU  - Ma X
AD  - Department of Ophthalmology, Shanghai University of Medicine & Health Sciences 
      Affiliated Zhoupu Hospital, Shanghai, China. Electronic address: 
      maxiaoy1820@126.com.
FAU - Zou, Jun
AU  - Zou J
AD  - Department of Ophthalmology, Shanghai Tenth People's Hospital, Tongji University, 
      Shanghai, China. Electronic address: zoujun70@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210610
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cornea/*innervation/metabolism/pathology
MH  - Follow-Up Studies
MH  - Humans
MH  - *Keratomileusis, Laser In Situ
MH  - Lasers, Excimer/*therapeutic use
MH  - Male
MH  - Metabolomics/*methods
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Myopia/diagnosis/metabolism/*surgery
MH  - Nerve Fibers/*physiology
MH  - Nerve Regeneration/*physiology
MH  - Prospective Studies
MH  - Time Factors
MH  - Young Adult
OTO - NOTNLM
OT  - Age
OT  - Corneal wound healing
OT  - Metabolite
OT  - Small incision lenticule extraction (SMILE)
EDAT- 2021/06/13 06:00
MHDA- 2021/12/01 06:00
CRDT- 2021/06/12 20:08
PHST- 2021/01/06 00:00 [received]
PHST- 2021/06/01 00:00 [revised]
PHST- 2021/06/07 00:00 [accepted]
PHST- 2021/06/13 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2021/06/12 20:08 [entrez]
AID - S0014-4835(21)00231-1 [pii]
AID - 10.1016/j.exer.2021.108665 [doi]
PST - ppublish
SO  - Exp Eye Res. 2021 Aug;209:108665. doi: 10.1016/j.exer.2021.108665. Epub 2021 Jun 
      10.