PMID- 34119802
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 259
DP  - 2021 Aug
TI  - Identification of potential SLA-I-restricted CTL epitopes within the M protein of 
      porcine reproductive and respiratory syndrome virus.
PG  - 109131
LID - S0378-1135(21)00154-1 [pii]
LID - 10.1016/j.vetmic.2021.109131 [doi]
AB  - CD8+ cytotoxic T lymphocytes (CTLs), are essential for clearance of porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection and regulation of 
      host immune responses. Identification of SLA I-restricted CD8+ CTL epitopes would 
      facilitate PRRSV vaccine development. Here, cells isolated from peripheral blood 
      mononuclear cells (PBMCs) of PRRSV-immunized Large White pigs (JXA1-R strain) 
      were screened for immunodominant PRRSV-2 M protein T cell epitopes via ELISPOT 
      assay. Of nine immunodominant epitopes detected, eight elicited significant IFN-gamma 
      secretion responses that varied among individual pigs and according to epitope. 
      To predict which epitopes harbored potential CTL epitopes, swine leukocyte 
      antigen (SLA) class I genes of Large White pigs were cloned and sequenced, 
      yielding fourteen distinct SLA class I gene sequences. Based on ELISPOT and SLA 
      genotyping results, SLA-restricted binding of the fourteen predicted class I 
      proteins to peptides derived from the eight immunodominant epitopes were 
      predicted in-silico. After evaluation of 42 pET-peptide-SLA-I-beta2m complexes 
      containing predicted restricted peptides, extracellular SLA class I domains and 
      beta2m, ELISA testing of 33 peptide-SLA-I-beta2m complexes detected four complexed 
      peptides. These four peptides were evaluated using in vitro complex refolding 
      assays that confirmed that M(2-5) and M(6-1) peptides each formed complexes with 
      SLA-2*0502 and sbeta2m, while M(9-1) formed a complex with SLA-2*1201 and sbeta2m. 
      ELISPOT results confirmed these three 9-mer potential CTL epitopes efficiently 
      stimulated IFN-gamma secretion when presented by SLA class I molecules specified 
      here. This study describes effective CTL epitope identification methods for use 
      in future investigations of swine cellular immunity toward T cell-based vaccine 
      development.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Liang, Chao
AU  - Liang C
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Xia, Qianhui
AU  - Xia Q
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Zhou, Jingming
AU  - Zhou J
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Chen, Yumei
AU  - Chen Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Liu, Yankai
AU  - Liu Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Ding, Peiyang
AU  - Ding P
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Qi, Yanhua
AU  - Qi Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China. 
      Electronic address: pingaw@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210526
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (swine leukocyte antigen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Computer Simulation
MH  - Epitopes, T-Lymphocyte/genetics/*immunology
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Immunodominant Epitopes/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Protein Binding
MH  - Swine
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Viral Matrix Proteins/*genetics/*immunology
OTO - NOTNLM
OT  - CTL epitopes
OT  - Non-glycosylated membrane protein
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Swine leukocyte antigen
EDAT- 2021/06/14 06:00
MHDA- 2021/11/05 06:00
CRDT- 2021/06/13 20:45
PHST- 2021/02/08 00:00 [received]
PHST- 2021/05/23 00:00 [accepted]
PHST- 2021/06/14 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/06/13 20:45 [entrez]
AID - S0378-1135(21)00154-1 [pii]
AID - 10.1016/j.vetmic.2021.109131 [doi]
PST - ppublish
SO  - Vet Microbiol. 2021 Aug;259:109131. doi: 10.1016/j.vetmic.2021.109131. Epub 2021 
      May 26.