PMID- 34121212
OWN - NLM
STAT- MEDLINE
DCOM- 20211116
LR  - 20211116
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 35
IP  - 11
DP  - 2021 Nov
TI  - Determination of bupivacaine tissue concentration in human biopsy samples using 
      high-performance liquid chromatography with mass spectrometry.
PG  - e5198
LID - 10.1002/bmc.5198 [doi]
AB  - In the present study, we developed a simple and rapid analytical method for the 
      quantification of bupivacaine hydrochloride in human biopsy samples of adipose, 
      muscle, neural, connective and cartilage tissue using liquid chromatography-mass 
      spectrometry. Anesthetics were extracted from the tissue samples using 0.1% 
      formic acid in acetonitrile for protein denaturation and hexane for removal of 
      lipophilic impurities. Analytes were separated adequately on Phenomenex Luna 
      Omega polar C18 column using a gradient mobile phase 0.1% formic acid in water 
      and 0.1% formic acid in acetonitrile. The lower limits of quantification were </= 
      97 ng g(-1) tissue for all studied tissues. Intra-day recoveries were between 
      48.2% and 82.1% with relative standard deviations (RSDs) between 1.47% and 
      14.28%, whereas inter-day recoveries were between 52.2% and 77.6% with RSDs 
      between 2.98% and 14.79%. The calibration curve showed a linear fit with R(2) 
      higher than 0.99 in the concentration range from 0.16 to 100 mug g(-1) . Lidocaine 
      hydrochloride was tested as internal standard because its recoveries and matrix 
      effects were comparable to bupivacaine hydrochloride. Post-analytical corrections 
      of measured bupivacaine tissue concentrations can accordingly be made based on 
      recovery of lidocaine as internal standard, with recoveries between 51.2% and 
      86.9% and RSDs between 1.99% and 16.88%. The developed method could be used to 
      study time-dependent spread of bupivacaine locally or to more distant locations 
      across tissue barriers.
CI  - (c) 2021 John Wiley & Sons, Ltd.
FAU - Istenic, Simon
AU  - Istenic S
AD  - Core Facility, Helios TBLUS, Domzale, Slovenia.
AD  - Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, 
      Slovenia.
FAU - Cvetko, Erika
AU  - Cvetko E
AD  - Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, 
      Slovenia.
FAU - Zabret, Jozi
AU  - Zabret J
AD  - Core Facility, Helios TBLUS, Domzale, Slovenia.
FAU - Stopar Pintaric, Tatjana
AU  - Stopar Pintaric T
AD  - Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, 
      Slovenia.
AD  - Department of Anaesthesiology and Intensive Therapy, University Medical Centre 
      Ljubljana, Ljubljana, Slovenia.
FAU - Umek, Nejc
AU  - Umek N
AUID- ORCID: 0000-0001-5831-2216
AD  - Institute of Anatomy, Faculty of Medicine, University of Ljubljana, Ljubljana, 
      Slovenia.
LA  - eng
GR  - Grant No: P3-0043/Slovenian Research Agency/
GR  - Tertiary funding/University Medical Center Ljubljana/
PT  - Journal Article
DEP - 20210704
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - Y8335394RO (Bupivacaine)
SB  - IM
MH  - Biopsy
MH  - Bupivacaine/*analysis/chemistry/isolation & purification
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Linear Models
MH  - Mass Spectrometry/*methods
MH  - Muscle, Skeletal/pathology
MH  - Nerve Tissue/pathology
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - HPLC-MS
OT  - biopsy sample
OT  - bupivacaine
OT  - human tissue
OT  - lidocaine
EDAT- 2021/06/15 06:00
MHDA- 2021/11/17 06:00
CRDT- 2021/06/14 06:59
PHST- 2021/05/13 00:00 [revised]
PHST- 2021/03/28 00:00 [received]
PHST- 2021/06/09 00:00 [accepted]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/11/17 06:00 [medline]
PHST- 2021/06/14 06:59 [entrez]
AID - 10.1002/bmc.5198 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2021 Nov;35(11):e5198. doi: 10.1002/bmc.5198. Epub 2021 Jul 4.