PMID- 34121238
OWN - NLM
STAT- MEDLINE
DCOM- 20211110
LR  - 20220531
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 50
IP  - 10
DP  - 2021 Nov
TI  - Wnt/beta-catenin signaling pathway participates in the effect of miR-626 on oral 
      squamous cell carcinoma by targeting RASSF4.
PG  - 1005-1017
LID - 10.1111/jop.13216 [doi]
AB  - BACKGROUND: The role of miR-626 in oral squamous cell carcinoma (OSCC) was 
      investigated by targeting RASSF4. METHODS: The miR-626 and RASSF4 expression was 
      detected in normal oral mucosa or OSCC tissues and OSCC or normal cells. The 
      methylation status of RASSF4 was analyzed using methylation-specific polymerase 
      chain reaction (PCR). The cytoplasmic/nuclear ratios (C/N ratios) targeted by 
      miR-626 were examined using microarray, followed by a dual-luciferase reporter 
      assay. The subcellular localization of RASSF4 and miR-626 in OSCC cells was 
      determined using RNA fluorescence in situ hybridization (FISH) and 
      immunocytochemistry (ICC), respectively. Ca9-22 and HSC2 cells were divided into 
      mock, inhibitor NC, miR-626 inhibitor, scramble, RASSF4 and miR-626 
      mimic + RASSF4 groups, and then CCK-8, Annexin V-FITC/PI, wound healing, 
      Transwell, qRT-PCR and western blotting assays were performed. RESULTS: OSCC 
      tissues and cells had increased miR-626 expression and decreased RASSF4 
      expression. Patients with RASSF4 methylation had lower RASSF4 expression than 
      those without methylation. In addition, a negative correlation between miR-626 
      and RASSF4 was found in OSCC tissues, both of which were correlated with the 
      pathological grade, pathological stage, lymph node metastasis and patient 
      prognosis. MiR-626 targeted RASSF4 in OSCC cells. Overexpressed RASSF4 inhibited 
      the proliferation, invasion, migration and epithelial-mesenchymal transition 
      (EMT) of OSCC cells, promoted cell apoptosis, and blocked the Wnt/beta-Catenin 
      pathway, which was reversed by miR-626 overexpression. CONCLUSIONS: Inhibiting 
      miR-626 can regulate the biological characteristics of OSCC cells, including 
      proliferation, invasion, migration, EMT and apoptosis, by targeting RASSF4, which 
      may be related to the Wnt/beta-Catenin pathway.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Cui, Sheng-Hai
AU  - Cui SH
AD  - Department of Oral and Maxillofacial Surgery, Yantai Stomatological Hospital, 
      Yantai, China.
FAU - Hu, Xiao-Di
AU  - Hu XD
AD  - Department of Stomatology, Yantaishan Hospital, Yantai, China.
FAU - Yan, Yan
AU  - Yan Y
AUID- ORCID: 0000-0002-7925-741X
AD  - Department of Stomatology, Yantaishan Hospital, Yantai, China.
LA  - eng
SI  - RefSeq/NM_032023
PT  - Journal Article
DEP - 20210628
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (MIRN626 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RASSF4 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - *Carcinoma, Squamous Cell/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - MicroRNAs/genetics/*metabolism
MH  - *Mouth Neoplasms/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Tumor Suppressor Proteins/genetics
MH  - Wnt Signaling Pathway/genetics
OTO - NOTNLM
OT  - OSCC
OT  - RASSF4
OT  - Wnt/beta-Catenin pathway
OT  - miR-626
EDAT- 2021/06/15 06:00
MHDA- 2021/11/11 06:00
CRDT- 2021/06/14 07:01
PHST- 2021/06/08 00:00 [revised]
PHST- 2021/04/12 00:00 [received]
PHST- 2021/06/10 00:00 [accepted]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/11/11 06:00 [medline]
PHST- 2021/06/14 07:01 [entrez]
AID - 10.1111/jop.13216 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2021 Nov;50(10):1005-1017. doi: 10.1111/jop.13216. Epub 2021 
      Jun 28.