PMID- 34121491
OWN - NLM
STAT- MEDLINE
DCOM- 20220307
LR  - 20220307
IS  - 1530-8022 (Electronic)
IS  - 0885-3282 (Linking)
VI  - 36
IP  - 5
DP  - 2021 Nov
TI  - Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer 
      network hydrogel for cartilage tissue engineering.
PG  - 803-817
LID - 10.1177/08853282211024020 [doi]
AB  - In the present study, alginate/cartilage extracellular matrix (ECM)-based 
      injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) 
      for cartilage tissue engineering. The in situ forming hydrogels were composed of 
      different ionic crosslinked alginate concentrations with 1% w/v enzymatically 
      crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer 
      network (IPN). The response surface methodology (RSM) approach was applied to 
      optimize IPN hydrogel's mechanical properties by varying alginate and SFN 
      concentrations. The results demonstrated that upon increasing the alginate 
      concentration, the compression modulus improved. The SFN concentration was 
      optimized to reach a desired mechanical stiffness. Accordingly, the 
      concentrations of alginate and SFN to have an optimum compression modulus in the 
      hydrogel were found to be 1.685 and 1.724% w/v, respectively. The gelation time 
      was found to be about 10 s for all the samples. Scanning electron microscope 
      (SEM) images showed homogeneous dispersion of the SFN in the hydrogel, mimicking 
      the natural cartilage environment. Furthermore, water uptake capacity, 
      degradation rate, cell cytotoxicity, and glycosaminoglycan and collagen II 
      secretions were determined for the optimum hydrogel to support its potential as 
      an injectable scaffold for articular cartilage defects.
FAU - Shojarazavi, Nastaran
AU  - Shojarazavi N
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran, Iran.
FAU - Mashayekhan, Shohreh
AU  - Mashayekhan S
AUID- ORCID: 0000-0002-5479-5625
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran, Iran.
FAU - Pazooki, Hossein
AU  - Pazooki H
AD  - Department of Chemical and Environmental Engineering, Amirkabir University of 
      Technology, Tehran, Iran.
FAU - Mohsenifard, Sadaf
AU  - Mohsenifard S
AD  - Department of Chemical and Petroleum Engineering, Sharif University of 
      Technology, Tehran, Iran.
FAU - Baniasadi, Hossein
AU  - Baniasadi H
AUID- ORCID: 0000-0002-0463-337X
AD  - Polymer Technology, School of Chemical Engineering, Aalto University, Espoo, 
      Finland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210614
PL  - England
TA  - J Biomater Appl
JT  - Journal of biomaterials applications
JID - 8813912
RN  - 0 (Alginates)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Hydrogels)
RN  - 0 (Polymers)
RN  - 9007-34-5 (Collagen)
RN  - 9007-76-5 (Fibroins)
SB  - IM
MH  - *Alginates
MH  - *Cartilage/drug effects
MH  - Cartilage, Articular
MH  - Chondrocytes/drug effects
MH  - Collagen/metabolism
MH  - *Extracellular Matrix/drug effects
MH  - Fibroins
MH  - Glycosaminoglycans
MH  - Hydrogels/*chemistry/pharmacology
MH  - Polymers/*chemistry/pharmacology
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Injectable hydrogels
OT  - alginate
OT  - cartilage tissue engineering
OT  - interpenetrating polymer network
EDAT- 2021/06/15 06:00
MHDA- 2022/03/08 06:00
CRDT- 2021/06/14 09:02
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2022/03/08 06:00 [medline]
PHST- 2021/06/14 09:02 [entrez]
AID - 10.1177/08853282211024020 [doi]
PST - ppublish
SO  - J Biomater Appl. 2021 Nov;36(5):803-817. doi: 10.1177/08853282211024020. Epub 
      2021 Jun 14.