PMID- 34122460
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210615
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Identifying Anti-Oxidant Biosynthesis Genes in Pearl Millet [Pennisetum glaucum 
      (L.) R. Br.] Using Genome-Wide Association Analysis.
PG  - 599649
LID - 10.3389/fpls.2021.599649 [doi]
LID - 599649
AB  - Pearl millet [Pennisetum glaucum (L.) R Br.] is an important staple food crop in 
      the semi-arid tropics of Asia and Africa. It is a cereal grain that has the 
      prospect to be used as a substitute for wheat flour for celiac patients. It is an 
      important antioxidant food resource present with a wide range of phenolic 
      compounds that are good sources of natural antioxidants. The present study aimed 
      to identify the total antioxidant content of pearl millet flour and apply it to 
      evaluate the antioxidant activity of its 222 genotypes drawn randomly from the 
      pearl millet inbred germplasm association panel (PMiGAP), a world diversity panel 
      of this crop. The total phenolic content (TPC) significantly correlated with DPPH 
      (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (% inhibition), which 
      ranged from 2.32 to 112.45% and ferric-reducing antioxidant power (FRAP) activity 
      ranging from 21.68 to 179.66 (mg ascorbic acid eq./100 g). Genome-wide 
      association studies (GWAS) were conducted using 222 diverse accessions and 67 K 
      SNPs distributed across all the seven pearl millet chromosomes. Approximately, 
      218 SNPs were found to be strongly associated with DPPH and FRAP activity at high 
      confidence [-log (p) > 3.0-7.4]. Furthermore, flanking regions of significantly 
      associated SNPs were explored for candidate gene harvesting. This identified 18 
      candidate genes related to antioxidant pathway genes (flavanone 
      7-O-beta-glycosyltransferase, GDSL esterase/lipase, glutathione S-transferase) 
      residing within or near the association signal that can be selected for further 
      functional characterization. Patterns of genetic variability and the associated 
      genes reported in this study are useful findings, which would need further 
      validation before their utilization in molecular breeding for high 
      antioxidant-containing pearl millet cultivars.
CI  - Copyright (c) 2021 Yadav, Tokas, Yadav, Winters, Singh, Yadav, Gangashetty, 
      Srivastava and Yadav.
FAU - Yadav, Chandra Bhan
AU  - Yadav CB
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
FAU - Tokas, Jayanti
AU  - Tokas J
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
AD  - Chaudhary Charan Singh (CCS) Haryana Agricultural University, Hisar, India.
FAU - Yadav, Devvart
AU  - Yadav D
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
AD  - Chaudhary Charan Singh (CCS) Haryana Agricultural University, Hisar, India.
FAU - Winters, Ana
AU  - Winters A
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
FAU - Singh, Ram B
AU  - Singh RB
AD  - International Crops Research Institute for the Semi-Arid Tropics, Patancheru, 
      India.
FAU - Yadav, Rama
AU  - Yadav R
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
FAU - Gangashetty, Prakash I
AU  - Gangashetty PI
AD  - International Crops Research Institute for the Semi-Arid Tropics, Niamey, Niger.
FAU - Srivastava, Rakesh K
AU  - Srivastava RK
AD  - International Crops Research Institute for the Semi-Arid Tropics, Patancheru, 
      India.
FAU - Yadav, Rattan S
AU  - Yadav RS
AD  - Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth 
      University, Aberystwyth, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20210528
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8194398
OTO - NOTNLM
OT  - antioxidant activity
OT  - candidate genes
OT  - genome-wide association study
OT  - germplasm
OT  - marker-trait associations
OT  - pearl millet [Pennisetum glaucum (L.) R. Br.]
OT  - phenolics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/15 06:00
MHDA- 2021/06/15 06:01
PMCR- 2021/01/01
CRDT- 2021/06/14 09:37
PHST- 2020/08/27 00:00 [received]
PHST- 2021/04/19 00:00 [accepted]
PHST- 2021/06/14 09:37 [entrez]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/06/15 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.599649 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 May 28;12:599649. doi: 10.3389/fpls.2021.599649. 
      eCollection 2021.