PMID- 34122596
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220423
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2021
DP  - 2021
TI  - Da-Huang-Fu-Zi-Tang Ameliorates Severe Acute Pancreatitis by Elevation of M2 
      Kupffer Cells in Rats.
PG  - 5561216
LID - 10.1155/2021/5561216 [doi]
LID - 5561216
AB  - INTRODUCTION: Severe acute pancreatitis (SAP) is a clinical emergency often 
      accompanied by inflammatory response syndrome (SIRS), which eventually leads to 
      acute lung injury and failure of other organs. The activation of liver Kupffer 
      cells (KCs) plays a major role in the process of SIRS and multiorgan damage 
      caused by SAP. Da-Huang-Fu-Zi-Tang (DHFZT), a traditional Chinese prescription, 
      has been widely used for SAP in the clinic. The present study investigated the 
      activation state of KCs in SAP and the potential mechanism of DHFZT. METHODS: A 
      total of 24 Sprague Dawley rats were randomly assigned to four groups: SH (sham 
      operation group + saline enema), SH-DHFZT (sham operation group + DHFZT enema), 
      SAP (model group + saline enema), and SAP-DHFZT (model group + DHFZT enema). 
      Blood samples were drawn from the abdominal aorta for measuring serum endotoxin, 
      amylase, calcium ion, IL-1beta, TNF-alpha, iNOS, and IL-10. Then, the pancreas, lung, 
      liver, and ileum were harvested for histological observation, and the liver was 
      used to detect the level of F4/80, CD86, and CD163 in KCs with 
      immunohistochemistry and western blot. RESULTS: In the SAP group, the CD86+ KCs 
      were significantly increased with a high level of IL-1beta, TNF-alpha, and iNOS, and the 
      organs were impaired. In the SAP-DHFZT group, CD163+ KCs were significantly 
      increased with the high level of IL-10, and the damage to organs was alleviated. 
      CONCLUSION: These phenomena suggested that the SIRS and multiple organ damage in 
      SAP might be related to the excessive activation of M1 KCs, and DHFZT might 
      alleviate the SIRS by inducing the differentiation of KCs into the M2-type and 
      promote the expression of anti-inflammatory factor IL-10.
CI  - Copyright (c) 2021 Yi Song et al.
FAU - Song, Yi
AU  - Song Y
AD  - Department of Emergency Medicine, The Affiliated Zhongshan Hospital of Dalian 
      University, Dalian 116001, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Department of Emergency Intensive Care Unit, Yiwu Central Hospital, Yiwu 322000, 
      China.
FAU - Qi, Xin
AU  - Qi X
AD  - Graduate School, Dalian University, Dalian 116622, China.
FAU - Kang, Xin
AU  - Kang X
AD  - Department of Emergency Medicine, The Affiliated Zhongshan Hospital of Dalian 
      University, Dalian 116001, China.
FAU - Lu, Xiaoguang
AU  - Lu X
AUID- ORCID: 0000-0002-0236-7178
AD  - Department of Emergency Medicine, The Affiliated Zhongshan Hospital of Dalian 
      University, Dalian 116001, China.
LA  - eng
PT  - Journal Article
DEP - 20210602
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC8192177
COIS- The authors declare no conflicts of interest.
EDAT- 2021/06/15 06:00
MHDA- 2021/06/15 06:01
PMCR- 2021/06/02
CRDT- 2021/06/14 09:38
PHST- 2021/02/06 00:00 [received]
PHST- 2021/05/08 00:00 [revised]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/06/14 09:38 [entrez]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/06/15 06:01 [medline]
PHST- 2021/06/02 00:00 [pmc-release]
AID - 10.1155/2021/5561216 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2021 Jun 2;2021:5561216. doi: 
      10.1155/2021/5561216. eCollection 2021.