PMID- 34123108
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210615
IS  - 2041-6520 (Print)
IS  - 2041-6539 (Electronic)
IS  - 2041-6520 (Linking)
VI  - 11
IP  - 32
DP  - 2020 Jul 23
TI  - A Janus 3D DNA nanomachine for simultaneous and sensitive fluorescence detection 
      and imaging of dual microRNAs in cancer cells.
PG  - 8482-8488
LID - 10.1039/d0sc02850a [doi]
AB  - Herein, a Janus three-dimensional (3D) DNA nanomachine was constructed for the 
      simultaneous and sensitive fluorescence detection and imaging of dual microRNAs 
      (miRNAs) in cancer cells, which could effectively eliminate signal interference 
      in a homogeneous nanoparticle-based 3D DNA nanostructure caused by the proximity 
      of the two different signal probes to achieve accurate co-location in the same 
      position of living cancer cells. In this system, the Janus nanoparticles were 
      synthesized as the carrier for immobilizing two different oligonucleotides on two 
      different functionalized hemispheres of the nanoparticles to form a Janus 3D DNA 
      nanostructure, which could convert trace amounts of miRNA-21 and miRNA-155 
      targets into massive FAM and Cy5-labeled duplexes to induce two remarkable 
      fluorescence emissions by the catalytic hairpin assembly (CHA) and 3D DNA walker 
      cascade nucleic acid amplification strategy, realizing sensitive detection and 
      imaging of miRNA targets in cancer cells. Impressively, in comparison with 
      current miRNA imaging methods based on nanoparticle assemblies, the proposed 
      strategy could efficiently eliminate "false positive" results obtained in single 
      type miRNA detection and distinctly increase the immobilization concentration of 
      two different signal probes using Janus nanoparticles as the carrier to further 
      enhance fluorescence intensity, resulting in accurate co-location in the same 
      position of living cells. Meanwhile, the proposed fluorescence imaging technology 
      makes it possible to visualize low concentrations of miRNAs with tiny change 
      associated with some cancers, which could significantly improve the accuracy and 
      precision compared to those of the conventional fluorescence in situ 
      hybridization (FISH) approach. Therefore, it could serve as persuasive evidence 
      for supplying accurate information to better understand biological processes and 
      investigate mechanisms of various biomolecules and subcellular organelles, 
      resulting in the further validation of their function in tumor proliferation and 
      differentiation. This strategy provided an innovative approach to design new 
      generations of nanomachines with ultimate applications in bioanalysis and 
      clinical diagnoses.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Yang, Zezhou
AU  - Yang Z
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Peng, Xin
AU  - Peng X
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Yang, Peng
AU  - Yang P
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Zhuo, Ying
AU  - Zhuo Y
AUID- ORCID: 0000-0001-7356-5924
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Chai, Ya-Qin
AU  - Chai YQ
AUID- ORCID: 0000-0003-4392-9592
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Liang, Wenbin
AU  - Liang W
AUID- ORCID: 0000-0002-9086-4974
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
FAU - Yuan, Ruo
AU  - Yuan R
AUID- ORCID: 0000-0003-3664-6236
AD  - Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest 
      University), Ministry of Education, College of Chemistry and Chemical 
      Engineering, Southwest University Chongqing 400715 PR China 
      wenbinliangasu@gmail.com yuanruo@swu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200723
PL  - England
TA  - Chem Sci
JT  - Chemical science
JID - 101545951
PMC - PMC8163441
COIS- There are no conflicts to declare.
EDAT- 2021/06/15 06:00
MHDA- 2021/06/15 06:01
PMCR- 2020/07/23
CRDT- 2021/06/14 09:42
PHST- 2021/06/14 09:42 [entrez]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/06/15 06:01 [medline]
PHST- 2020/07/23 00:00 [pmc-release]
AID - d0sc02850a [pii]
AID - 10.1039/d0sc02850a [doi]
PST - epublish
SO  - Chem Sci. 2020 Jul 23;11(32):8482-8488. doi: 10.1039/d0sc02850a.