PMID- 34124373
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220423
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 16
IP  - 1
DP  - 2021
TI  - Hyperhomocysteinemia exacerbates ischemia-reperfusion injury-induced acute kidney 
      injury by mediating oxidative stress, DNA damage, JNK pathway, and apoptosis.
PG  - 537-543
LID - 10.1515/biol-2021-0054 [doi]
AB  - BACKGROUND: Hyperhomocysteinemia (HHcy) plays an important role in the 
      progression of many kidney diseases; however, the relationship between HHcy and 
      ischemia-reperfusion injury (IRI)-induced acute kidney injury (IRI-induced AKI) 
      is far from clear. In this study, we try to investigate the effect and possible 
      mechanisms of HHcy on IRI-induced AKI. METHODS: Twenty C57/BL6 mice were reared 
      with a regular diet or high methionine diet for 2 weeks (to generate HHcy mice); 
      after that, mice were subgrouped to receive sham operation or 
      ischemia-reperfusion surgery. Twenty four hour after reperfusion, serum 
      creatinine, blood urea nitrogen, and Malondialdehyde (MDA) were measured. H&E 
      staining for tubular injury, western blot for gammaH2AX, JNK, p-JNK, and cleaved 
      caspase 3, and TUNEL assay for tubular cell apoptosis were also performed. 
      RESULTS: Our results showed that HHcy did not influence the renal function and 
      histological structure, as well as the levels of MDA, gammaH2AX, JNK, p-JNK, and 
      tubular cell apoptosis in control mice. However, in IRI-induced AKI mice, HHcy 
      caused severer renal dysfunction and tubular injury, higher levels of oxidative 
      stress, DNA damage, JNK pathway activation, and tubular cell apoptosis. 
      CONCLUSION: Our results demonstrated that HHcy could exacerbate IRI-induced AKI, 
      which may be achieved through promoting oxidative stress, DNA damage, JNK pathway 
      activation, and consequent apoptosis.
CI  - (c) 2021 Mei Zhang et al., published by De Gruyter.
FAU - Zhang, Mei
AU  - Zhang M
AD  - Department of Biomedicine, Guizhou University School of Medicine, Guizhou 
      University, Guiyang 550025, Guizhou, People's Republic of China.
FAU - Yuan, Jing
AU  - Yuan J
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Dong, Rong
AU  - Dong R
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Da, Jingjing
AU  - Da J
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Li, Qian
AU  - Li Q
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Hu, Ying
AU  - Hu Y
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Yu, Fangfang
AU  - Yu F
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Ran, Yan
AU  - Ran Y
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Zha, Yan
AU  - Zha Y
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
FAU - Long, Yanjun
AU  - Long Y
AD  - Division of Nephrology, Guizhou Provincial People's Hospital, Guizhou Provincial 
      Institute of Nephritic & Urinary Disease, No. 83 East ZhongShan Road, Guiyang 
      550002, Guizhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210529
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC8165256
OTO - NOTNLM
OT  - JNK signaling pathway
OT  - acute kidney injury
OT  - hyperhomocysteinemia
OT  - ischemia-reperfusion injury
OT  - oxidative stress
COIS- Conflict of interest: The authors state no conflict of interest.
EDAT- 2021/06/15 06:00
MHDA- 2021/06/15 06:01
PMCR- 2021/05/29
CRDT- 2021/06/14 09:54
PHST- 2020/11/09 00:00 [received]
PHST- 2021/04/01 00:00 [revised]
PHST- 2021/04/22 00:00 [accepted]
PHST- 2021/06/14 09:54 [entrez]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/06/15 06:01 [medline]
PHST- 2021/05/29 00:00 [pmc-release]
AID - biol-2021-0054 [pii]
AID - 10.1515/biol-2021-0054 [doi]
PST - epublish
SO  - Open Life Sci. 2021 May 29;16(1):537-543. doi: 10.1515/biol-2021-0054. 
      eCollection 2021.