PMID- 34124974
OWN - NLM
STAT- MEDLINE
DCOM- 20211130
LR  - 20211130
IS  - 1365-2060 (Electronic)
IS  - 0785-3890 (Print)
IS  - 0785-3890 (Linking)
VI  - 53
IP  - 1
DP  - 2021 Dec
TI  - Hsa_circ_0042823 accelerates cancer progression via miR-877-5p/FOXM1 axis in 
      laryngeal squamous cell carcinoma.
PG  - 960-970
LID - 10.1080/07853890.2021.1934725 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumour 
      of the head and neck. Our previous study reveals that the circular RNA (circRNA) 
      hsa_circ_0042823 is abnormally expressed in LSCC, suggesting that 
      hsa_circ_0042823 is closely associated with LSCC. Here, we attempted to explore 
      the molecular mechanism of hsa_circ_0042823 in LSCC. METHODS: Quantitative 
      real-time PCR and western blot were performed to assess the expression of gene 
      and protein in human laryngeal carcinoma cells, TU212 and TU686. MTT and 
      transwell assays were performed to examine cell proliferation, migration and 
      invasion. The relationship among hsa_circ_0042823, miR-877-5p and forkhead box M1 
      (FOXM1) was verified by luciferase reporter assay. Finally, we constructed a 
      subcutaneous tumour mouse model to analyse in vivo growth of LSCC cells following 
      knockdown of hsa_circ_0042823. RESULTS: Compared with normal human bronchial 
      epithelial cells (HBECs), hsa_circ_0042823 was highly expressed in the LSCC cell 
      lines (AMC-HN-8 and TU686). Further studies demonstrated that hsa_circ_0042823 
      interacted with miR-877-5p, and FOXM1 was the target of miR-877-5p. 
      Hsa_circ_0042823 promoted the expression of FOXM1 via its ceRNA activity on 
      miR-877-5p. Hsa_circ_0042823 overexpression promoted proliferation, migration and 
      invasion of AMC-HN-8 cells through regulating miR-877-5p/FOXM1 axis. 
      Additionally, inhibition of hsa_circ_0042823 inhibited growth of LSCC in vivo via 
      miR-877-5p/FOXM1 axis. CONCLUSIONS: Hsa_circ_0042823/miR-877-5p/FOXM1 axis 
      participates in the progression of LSCC. This work demonstrates that 
      hsa_circ_0042823 accelerates cancer progression by regulating miR-877-5p/FOXM1 
      axis in LSCC. Therefore, this study may provide new insights into the 
      pathogenesis of LSCC.KEY MESSAGESHsa_circ_0042823 promotes FOXM1 expression by 
      sponging miR-877-5p.Hsa_circ_0042823 promotes proliferation, migration, invasion 
      of LSCC cells.Hsa_circ_0042823 knockdown inhibits tumour growth of LSCC via 
      miR-877-5p/FOXM1 axis.
FAU - Wu, Tianyi
AU  - Wu T
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, PR China.
FAU - Sun, Zhanwei
AU  - Sun Z
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
FAU - Li, Shichao
AU  - Li S
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
FAU - Wang, Weiwei
AU  - Wang W
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
FAU - Yu, Boyu
AU  - Yu B
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
FAU - Wang, Guangke
AU  - Wang G
AD  - Department of Otolaryngology, Head and Neck Surgery, Henan Provincial People's 
      Hospital, People's Hospital of Zhengzhou University; People's Hospital of Henan 
      University, Zhengzhou, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Med
JT  - Annals of medicine
JID - 8906388
RN  - 0 (FOXM1 protein, human)
RN  - 0 (Forkhead Box Protein M1)
RN  - 0 (MIRN877 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - *Forkhead Box Protein M1/genetics
MH  - *Head and Neck Neoplasms/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics
MH  - Mice
MH  - *MicroRNAs/genetics
MH  - RNA, Circular
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics
PMC - PMC8204964
OTO - NOTNLM
OT  - Circular RNA
OT  - FOXM1
OT  - hsa_circ_0042823
OT  - invasion
OT  - laryngeal squamous cell carcinoma
OT  - miR-877-5p
OT  - migration
OT  - proliferation
OT  - tumour growth
COIS- All authors declare no conflict of interest.
EDAT- 2021/06/15 06:00
MHDA- 2021/12/01 06:00
PMCR- 2021/06/14
CRDT- 2021/06/14 12:21
PHST- 2021/06/14 12:21 [entrez]
PHST- 2021/06/15 06:00 [pubmed]
PHST- 2021/12/01 06:00 [medline]
PHST- 2021/06/14 00:00 [pmc-release]
AID - 1934725 [pii]
AID - 10.1080/07853890.2021.1934725 [doi]
PST - ppublish
SO  - Ann Med. 2021 Dec;53(1):960-970. doi: 10.1080/07853890.2021.1934725.