PMID- 34130645
OWN - NLM
STAT- MEDLINE
DCOM- 20211019
LR  - 20211019
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jun 15
TI  - Identifying prognostic lncRNAs based on a ceRNA regulatory network in laryngeal 
      squamous cell carcinoma.
PG  - 705
LID - 10.1186/s12885-021-08422-2 [doi]
LID - 705
AB  - PURPOSE: Growing evidence demonstrates that long non-coding RNAs (lncRNAs) play a 
      crucial role as competing endogenous RNAs (ceRNAs) in tumor occurrence. The 
      lncRNAs' functions and clinical significance in laryngeal squamous cell carcinoma 
      (LSCC) remain unclear. The study aims to reveal the lncRNA-associated ceRNA 
      regulatory network of LSCC and clarify its clinical relevance. METHODS: Here, we 
      obtained LSCC transcriptome data from The Cancer Genome Atlas (TCGA) database and 
      identified the differential expression profile of lncRNAs, miRNAs, and mRNAs by 
      the EdgeR R package. The function enrichment analysis of mRNAs was performed 
      using clusterProfiler R package and GSEA3.0. Then, we constructed a ceRNA network 
      and prognosis model based on lncRNAs through bioinformatic methods. Moreover, we 
      explored the functions of prognosis-related lncRNA in LSCC by CCK-8 and transwell 
      assay. RESULTS: 1961 lncRNAs, 69 miRNAs, and 2224 mRNAs were identified as 
      differentially expressed genes in LSCC tissues. According to the transcriptome 
      differential expression profile, a ceRNA network containing 61 lncRNAs, 21 
      miRNAs, and 77 mRNAs was established. Then, four lncRNAs (AC011933.2, FAM30A, 
      LINC02086, LINC02575) were identified from the ceRNA network to build a prognosis 
      model for LSCC patients. And we found that LINC02086 and LINC02575 promoted the 
      proliferation, migration, and invasion of LSCC cells while AC011933.2 and FAM30A 
      inhibited these biological functions in vitro. Furthermore, we validated that 
      LINc02086/miR-770-5p/SLC26A2 axis promoted migration in LSCC. CONCLUSION: Four 
      lncRNAs of the ceRNA network were abnormally expressed and related to patient 
      prognosis in LSCC. They played a significant role in the progress of LSCC via 
      affecting the proliferation and metastasis of tumor cells.
FAU - Shi, Yong
AU  - Shi Y
AD  - Department of Reproductive Medicine Center of the First Affiliated Hospital of 
      Zhengzhou University, Zhengzhou University, Zhengzhou, 450000, China. 
      biosy1993@163.com.
FAU - Yang, Dongli
AU  - Yang D
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Hospital of 
      Shanxi Medical University, Shanxi Medical University, Taiyuan, China.
FAU - Qin, Yixiao
AU  - Qin Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Hospital of 
      Shanxi Medical University, Shanxi Medical University, Taiyuan, China.
LA  - eng
PT  - Journal Article
DEP - 20210615
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/mortality/pathology
MH  - Cell Movement
MH  - Female
MH  - Gene Regulatory Networks/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/mortality/pathology
MH  - Male
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
MH  - Transfection
PMC - PMC8207764
OTO - NOTNLM
OT  - CeRNA
OT  - Laryngeal squamous cell carcinoma
OT  - LncRNA
OT  - Prognosis
COIS- The authors declare that there is no conflict of interest.
EDAT- 2021/06/17 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/06/15
CRDT- 2021/06/16 05:43
PHST- 2021/01/14 00:00 [received]
PHST- 2021/05/25 00:00 [accepted]
PHST- 2021/06/16 05:43 [entrez]
PHST- 2021/06/17 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/06/15 00:00 [pmc-release]
AID - 10.1186/s12885-021-08422-2 [pii]
AID - 8422 [pii]
AID - 10.1186/s12885-021-08422-2 [doi]
PST - epublish
SO  - BMC Cancer. 2021 Jun 15;21(1):705. doi: 10.1186/s12885-021-08422-2.