PMID- 34131437
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210617
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 2
DP  - 2021 Aug
TI  - Activation of the NLRC4 inflammasome in renal tubular epithelial cell injury in 
      diabetic nephropathy.
PG  - 814
LID - 10.3892/etm.2021.10246 [doi]
LID - 814
AB  - Renal tubular interstitial injury plays a key role in the progression of diabetic 
      nephropathy (DN) and, thus, the study of renal tubular injury in DN is important. 
      The aim of the present study was to elucidate the role of the NLR family CARD 
      domain containing 4 (NLRC4) inflammasome in renal tubular epithelial cell (RTEC) 
      injury in DN. Human kidney biopsy tissues were obtained from patients with DN, 
      and normal kidney tissues were obtained from nephrectomies performed for renal 
      hamartoma. Human RTECs (HK2 cells) were divided into normal glucose (D-glucose 
      5.6 mmol/l), high glucose (HG; 30 mmol/l), high osmotic (D-glucose 5.6 mmol/l + 
      D-mannitol 24.4 mmol/l), HG + NLRC4 small interfering (si)RNA or HG + siRNA 
      control groups. Then, the expression levels of NLRC4, PTEN-induced kinase 1 
      (PINK1) and parkin, as well as the levels of mitochondrial reactive oxygen 
      species, which are associated with mitophagy, were observed. The expression 
      levels of NLRC4, PINK1, parkin and phosphorylated parkin in the RTECs of patients 
      with DN were higher compared with those in normal controls. In HK2 cells, HG 
      stimulated the expression of NLRC4, the secretion of IL-1beta and IL-18 and cell 
      death. Moreover, knockdown of NLRC4 expression in HK2 cells treated with HG 
      reduced the secretion of the inflammatory cytokines, IL-1beta and IL-18. The 
      findings of the present study may provide a rationale for the development of 
      treatments for patients with DN by preventing inflammasome activation.
CI  - Copyright: (c) Wang et al.
FAU - Wang, Yulin
AU  - Wang Y
AD  - Department of Nephrology, Zhengzhou University, Zhengzhou, Henan 450000, P.R. 
      China.
FAU - Gou, Rong
AU  - Gou R
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
FAU - Yu, Lu
AU  - Yu L
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
FAU - Wang, Liuwei
AU  - Wang L
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
FAU - Yang, Zijun
AU  - Yang Z
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
FAU - Guo, Yanhong
AU  - Guo Y
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
FAU - Tang, Lin
AU  - Tang L
AD  - Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, Henan 450052, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210528
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8193214
OTO - NOTNLM
OT  - NLRC4 inflammasome
OT  - diabetic nephropathy
OT  - mitophagy
OT  - renal tubular epithelial cells
COIS- The authors declare that they have no competing interests.
EDAT- 2021/06/17 06:00
MHDA- 2021/06/17 06:01
PMCR- 2021/05/28
CRDT- 2021/06/16 06:47
PHST- 2020/12/22 00:00 [received]
PHST- 2021/05/04 00:00 [accepted]
PHST- 2021/06/16 06:47 [entrez]
PHST- 2021/06/17 06:00 [pubmed]
PHST- 2021/06/17 06:01 [medline]
PHST- 2021/05/28 00:00 [pmc-release]
AID - ETM-0-0-10246 [pii]
AID - 10.3892/etm.2021.10246 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Aug;22(2):814. doi: 10.3892/etm.2021.10246. Epub 2021 May 28.