PMID- 34131588
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240402
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 16
IP  - 1
DP  - 2021
TI  - Potential prognostic markers and significant lncRNA-mRNA co-expression pairs in 
      laryngeal squamous cell carcinoma.
PG  - 544-557
LID - 10.1515/biol-2021-0052 [doi]
AB  - lncRNA-mRNA co-expression pairs and prognostic markers related to the development 
      of laryngeal squamous cell carcinoma (LSCC) were investigated. The lncRNA and 
      mRNA expression data of LSCC in GSE84957 and RNA-seq data of 112 LSCC samples 
      from TCGA database were used. Differentially expressed genes (DEGs) and lncRNAs 
      (DE-lncRNAs) between LSCC and para-cancer tissues were identified. Co-expression 
      analysis of DEGs and DE-lncRNA was conducted. Protein-protein interaction network 
      for co-expressed DEGs of top 25 DE-lncRNA was constructed, followed by survival 
      analysis for key nodes in co-expression network. Finally, expressions of several 
      DE-lncRNAs and DEGs were verified using qRT-PCR. The lncRNA-mRNA network showed 
      that ANKRD20A5P, C21orf15, CYP4F35P, LOC_I2_011146, XLOC_006053, XLOC_I2_003881, 
      and LOC100506027 were highlighted in network. Some DEGs, including FUT7, PADI1, 
      PPL, ARHGAP40, MUC21, and CEACAM1, were co-expressed with above lncRNAs. Survival 
      analysis showed that PLOD1, GLT25D1, and KIF22 were significantly associated with 
      prognosis. qRT-PCR results showed that the expressions of MUC21, CEACAM1, FUT7, 
      PADI1, PPL, ARHGAP40, ANKRD20A5P, C21orf15, CYP4F35P, XLOC_I2_003881, 
      LOC_I2_011146, and XLOC_006053 were downregulated, whereas the expression of 
      LOC100506027 was upregulated in LSCC tissues. PLOD1, GLT25D1, and KIF22 may be 
      potential prognostic markers in the development of LSCC. 
      C21orf15-MUC21/CEACAM1/FUT7/PADI1/PPL/ARHGAP40 are potential lncRNA-mRNA pairs 
      that play significant roles in the development of LSCC.
CI  - (c) 2021 Junguo Wang et al., published by De Gruyter.
FAU - Wang, Junguo
AU  - Wang J
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Department of Otolaryngology, Research Institute of Otolaryngology, No. 321 
      Zhongshan Road, Nanjing, 210008, China.
FAU - Liu, Dingding
AU  - Liu D
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Department of Otolaryngology, Research Institute of Otolaryngology, No. 321 
      Zhongshan Road, Nanjing, 210008, China.
FAU - Gu, Yajun
AU  - Gu Y
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Department of Otolaryngology, Research Institute of Otolaryngology, No. 321 
      Zhongshan Road, Nanjing, 210008, China.
FAU - Zhou, Han
AU  - Zhou H
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Department of Otolaryngology, Research Institute of Otolaryngology, No. 321 
      Zhongshan Road, Nanjing, 210008, China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Department of Otolaryngology, Research Institute of Otolaryngology, No. 321 
      Zhongshan Road, Nanjing, 210008, China.
FAU - Shen, Xiaohui
AU  - Shen X
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Research Institute of Otolaryngology, No. 321 Zhongshan Road, Nanjing, 210008, 
      China.
FAU - Qian, Xiaoyun
AU  - Qian X
AD  - Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower 
      Hospital of Nanjing University Medical School, Jiangsu Provincial Key Medical 
      Discipline (Laboratory), No. 321 Zhongshan Road, Nanjing, 210008, China.
AD  - Research Institute of Otolaryngology, No. 321 Zhongshan Road, Nanjing, 210008, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210602
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC8174121
OTO - NOTNLM
OT  - co-expression analysis
OT  - differential expression
OT  - laryngeal squamous cell carcinoma
OT  - lncRNA-mRNA
OT  - prognosis
COIS- Conflict of interest: The authors state no conflict of interest.
EDAT- 2021/06/17 06:00
MHDA- 2021/06/17 06:01
PMCR- 2021/06/02
CRDT- 2021/06/16 06:49
PHST- 2020/10/18 00:00 [received]
PHST- 2021/03/03 00:00 [revised]
PHST- 2021/04/13 00:00 [accepted]
PHST- 2021/06/16 06:49 [entrez]
PHST- 2021/06/17 06:00 [pubmed]
PHST- 2021/06/17 06:01 [medline]
PHST- 2021/06/02 00:00 [pmc-release]
AID - biol-2021-0052 [pii]
AID - 10.1515/biol-2021-0052 [doi]
PST - epublish
SO  - Open Life Sci. 2021 Jun 2;16(1):544-557. doi: 10.1515/biol-2021-0052. eCollection 
      2021.