PMID- 34136664
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210619
IS  - 2405-5808 (Electronic)
IS  - 2405-5808 (Linking)
VI  - 27
DP  - 2021 Sep
TI  - Consumption of barley ameliorates the diabetic steatohepatitis and reduces the 
      high transforming growth factor beta expression in mice grown in alpha-minimum essential 
      medium in vitro as embryos.
PG  - 101029
LID - 10.1016/j.bbrep.2021.101029 [doi]
LID - 101029
AB  - Non-alcoholic fatty liver disease (NAFLD), which includes the subtype 
      non-alcoholic steatohepatitis (NASH), is a major complication of type 2 diabetic 
      mellitus (T2DM), even among non-obese patients. However, the exact cause of 
      NAFLD/NASH in non-obese patients with T2DM is unclear. We studied a non-obese 
      mouse model of T2DM created through the malnourishment of embryos by culture in 
      vitro for 48 h in alpha-minimum essential medium (MEM) at the two-cell stage. We 
      compared the development of steatohepatitis in these MEM mice with control mice 
      that were similarly cultured in standard potassium simplex-optimized medium 
      (KSOM). We also studied the effects of 10 weeks of consumption of barley, which 
      contains large amounts of the soluble fiber beta-glucan, on the steatohepatitis of 
      the adult MEM mice. The size of lipid droplets, the area of fibrosis, and the 
      mRNA expression of the transforming growth factor beta (Tgfb) gene in the liver 
      were higher in adult MEM mice fed a rice-based diet than in KSOM mice fed the 
      same diet. However, barley consumption reduced the area of fibrosis and TGFB 
      expression in MEM mice. In conclusion, adult mice that are cultured in MEM at the 
      two-cell embryo stage develop steatohepatitis and T2DM, accompanied by higher 
      hepatic TGFB expression, than KSOM controls. Furthermore, the consumption of 
      barley during adulthood ameliorates the steatohepatitis and reduces the TGFB 
      expression.
CI  - (c) 2021 The Authors. Published by Elsevier B.V.
FAU - Ishiyama, Shiori
AU  - Ishiyama S
AD  - Department of Integrated Applied Life Science, Integrated Graduate School of 
      Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Kimura, Mayu
AU  - Kimura M
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Umihira, Nodoka
AU  - Umihira N
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Matsumoto, Sachi
AU  - Matsumoto S
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Takahashi, Atsushi
AU  - Takahashi A
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Nakagawa, Takao
AU  - Nakagawa T
AD  - Kiwa Laboratory Animals Co., Ltd., 486 Kebaramiya, Kiminocho, Wakayama, 640-1473, 
      Japan.
FAU - Wakayama, Teruhiko
AU  - Wakayama T
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
AD  - Advanced Biotechnology Center, University of Yamanashi, 4-4-37 Takeda, Kofu, 
      Yamanashi, 400-8510, Japan.
AD  - Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Kishigami, Satoshi
AU  - Kishigami S
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
AD  - Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi, 400-8510, Japan.
FAU - Mochizuki, Kazuki
AU  - Mochizuki K
AD  - Department of Integrated Applied Life Science, Integrated Graduate School of 
      Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi, 400-8510, Japan.
AD  - Graduate School of Life and Environmental Sciences, University of Yamanashi, 
      4-4-37 Takeda, Kofu, Yamanashi, 400-8510, Japan.
AD  - Faculty of Life and Environmental Sciences, University of Yamanashi, 4-4-37 
      Takeda, Kofu, Yamanashi, 400-8510, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210530
PL  - Netherlands
TA  - Biochem Biophys Rep
JT  - Biochemistry and biophysics reports
JID - 101660999
PMC - PMC8181192
OTO - NOTNLM
OT  - Barley
OT  - Fibrosis
OT  - Minimal essential medium
OT  - Non-alcoholic fatty liver disease
OT  - Non-alcoholic steatohepatitis
OT  - Transforming growth factor beta
EDAT- 2021/06/18 06:00
MHDA- 2021/06/18 06:01
PMCR- 2021/05/30
CRDT- 2021/06/17 06:50
PHST- 2020/12/22 00:00 [received]
PHST- 2021/05/06 00:00 [revised]
PHST- 2021/05/17 00:00 [accepted]
PHST- 2021/06/17 06:50 [entrez]
PHST- 2021/06/18 06:00 [pubmed]
PHST- 2021/06/18 06:01 [medline]
PHST- 2021/05/30 00:00 [pmc-release]
AID - S2405-5808(21)00123-0 [pii]
AID - 101029 [pii]
AID - 10.1016/j.bbrep.2021.101029 [doi]
PST - epublish
SO  - Biochem Biophys Rep. 2021 May 30;27:101029. doi: 10.1016/j.bbrep.2021.101029. 
      eCollection 2021 Sep.