PMID- 34139397
OWN - NLM
STAT- MEDLINE
DCOM- 20210819
LR  - 20210819
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 237
DP  - 2021 Aug
TI  - Developmental exposure to a POPs mixture or PFOS increased body weight and 
      reduced swimming ability but had no effect on reproduction or behavior in 
      zebrafish adults.
PG  - 105882
LID - S0166-445X(21)00141-7 [pii]
LID - 10.1016/j.aquatox.2021.105882 [doi]
AB  - Complex mixtures of persistent organic pollutants (POPs) are regularly detected 
      in the environment and animal tissues. Often these chemicals are associated with 
      latent effects following early-life exposures, following the developmental origin 
      of health and disease paradigm. We investigated the long-term effects of a human 
      relevant mixture of 29 POPs on adult zebrafish following a developmental 
      exposure, in addition to a single PFOS exposure for comparison, as it was the 
      compound with the highest concentration within the mixture. Zebrafish embryos 
      were exposed from 6 to 96 h post fertilization to x10 and x70 the level of POP 
      mixture or PFOS (0.55 and 3.83 muM) found in human blood before being transferred 
      to clean water. We measured growth, swimming performance, and reproductive output 
      at different life stages. In addition, we assessed anxiety behavior of the adults 
      and their offspring, as well as performing a transcriptomic analysis on the adult 
      zebrafish brain, as the POP mixture and PFOS concentrations used are known to 
      affect larval behavior. Exposure to POP mixture and PFOS reduced swimming 
      performance and increased length and weight, compared to controls. No effect of 
      developmental exposure was observed on reproductive output or anxiety behavior. 
      Additionally, RNA-seq did not reveal pathways related to anxiety although 
      pathways related to synapse biology were affected at the x10 PFOS level. 
      Furthermore, pathway analysis of the brain transcriptome of adults exposed as 
      larvae to the low concentration of PFOS revealed enrichment in pathways such as 
      calcium, MAPK, and GABA signaling, all of which are important for learning and 
      memory. Based on our results we can conclude that some effects on the endpoints 
      measured were apparent, but if these effects lead to adversities at population 
      levels remains elusive.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Christou, Maria
AU  - Christou M
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, P.O Box 369 Sentrum, 0102 Oslo, 
      Norway. Electronic address: mchristou_1987@hotmail.com.
FAU - Ropstad, Erik
AU  - Ropstad E
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, P.O Box 369 Sentrum, 0102 Oslo, 
      Norway. Electronic address: erik.ropstad@nmbu.no.
FAU - Brown, Stephen
AU  - Brown S
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, P.O Box 369 Sentrum, 0102 Oslo, 
      Norway. Electronic address: brownstephen323@gmail.com.
FAU - Kamstra, Jorke H
AU  - Kamstra JH
AD  - Faculty of Veterinary Medicine, Institute for Risk Assessment Sciences, Utrecht 
      University, 3584 CM Utrecht, the Netherlands. Electronic address: 
      j.h.kamstra@uu.nl.
FAU - Fraser, Thomas W K
AU  - Fraser TWK
AD  - Department of Production Animal Clinical Sciences, Faculty of Veterinary 
      Medicine, Norwegian University of Life Sciences, P.O Box 369 Sentrum, 0102 Oslo, 
      Norway. Electronic address: thomas.fraser@hi.no.
LA  - eng
PT  - Journal Article
DEP - 20210604
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - *Alkanesulfonic Acids/toxicity
MH  - Animals
MH  - Body Weight
MH  - *Environmental Pollutants
MH  - *Fluorocarbons/toxicity
MH  - Humans
MH  - Reproduction
MH  - Swimming
MH  - *Water Pollutants, Chemical/toxicity
MH  - Zebrafish
OTO - NOTNLM
OT  - Behavior
OT  - Growth
OT  - Pathway analysis
OT  - RNA-seq
OT  - Reproduction
OT  - U(crit)
EDAT- 2021/06/18 06:00
MHDA- 2021/08/20 06:00
CRDT- 2021/06/17 20:15
PHST- 2020/12/16 00:00 [received]
PHST- 2021/05/27 00:00 [revised]
PHST- 2021/05/31 00:00 [accepted]
PHST- 2021/06/18 06:00 [pubmed]
PHST- 2021/08/20 06:00 [medline]
PHST- 2021/06/17 20:15 [entrez]
AID - S0166-445X(21)00141-7 [pii]
AID - 10.1016/j.aquatox.2021.105882 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2021 Aug;237:105882. doi: 10.1016/j.aquatox.2021.105882. Epub 2021 
      Jun 4.