PMID- 34142871
OWN - NLM
STAT- MEDLINE
DCOM- 20211011
LR  - 20211011
IS  - 1557-8682 (Electronic)
IS  - 1527-0297 (Linking)
VI  - 22
IP  - 3
DP  - 2021 Sep
TI  - Impact of Hypoxic Exercise Recovery on Skeletal Muscle Glycogen and Gene 
      Expression.
PG  - 300-307
LID - 10.1089/ham.2021.0028 [doi]
AB  - Slivka, Dustin, Charles Dumke, Walter Hailes, and Brent Ruby. Impact of hypoxic 
      exercise recovery on skeletal muscle glycogen and gene expression. High Alt Med 
      Biol. 22:300-307, 2021. Background: The impact of altitude during recovery from 
      exercise is largely unknown. The purpose of this study was to determine the acute 
      gene response and muscle glycogen re-synthesis after exercise when exposed to 
      simulated high altitude during recovery. Materials and Methods: Twelve male 
      participants (age, 25 +/- 2 years; height, 178 +/- 7 cm; weight, 78.8 +/- 7.8 kg; 
      VO(2)peak, 4.25 +/- 0.59 l/min; Wpeak 307 +/- 44 W; and body fat, 13.1% +/- 1.2%) 
      completed two trials (random order), which consisted of cycling for 90 minutes in 
      laboratory conditions and then recovering for 6 hours in laboratory conditions 
      (975 m; normoxia) or at a high simulated altitude (5,000 m; hypoxia). Results: 
      Skeletal muscle biopsies from the vastus lateralis were obtained before exercise, 
      after exercise, and 6 hours after exercise for the measurement of metabolic gene 
      expression and muscle glycogen. Muscle glycogen decreased with exercise 
      (61% +/- 13%, p < 0.05) and increased with recovery (78% +/- 35%, p < 0.05) with no 
      difference between trials (p > 0.05). Hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, 
      optic atrophy gene 1 (OPA-1), mitofusin 2 (MFN-2), and peroxisome 
      proliferator-activated receptor gamma coactivator 1 alpha (PGC-1alpha) gene 
      expression were suppressed after altitude exposure (p < 0.05), while 
      mitochondrial fission 1 protein (FIS-1), phosphofructokinase (PFK), Cytochrome c 
      oxidase (COX), and hexokinase (HK) were unaffected by altitude exposure 
      (p > 0.05). Conclusions: High-altitude exposure during recovery from exercise 
      inhibits gene expression associated with mitochondrial development without 
      affecting muscle glycogen re-synthesis.
FAU - Slivka, Dustin
AU  - Slivka D
AUID- ORCID: 0000-0001-7337-3770
AD  - School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, 
      Nebraska, USA.
FAU - Dumke, Charles
AU  - Dumke C
AD  - School of Integrative Physiology and Athletic Training, University of Montana, 
      Missoula, Montana, USA.
FAU - Hailes, Walter
AU  - Hailes W
AD  - School of Integrative Physiology and Athletic Training, University of Montana, 
      Missoula, Montana, USA.
FAU - Ruby, Brent
AU  - Ruby B
AD  - School of Integrative Physiology and Athletic Training, University of Montana, 
      Missoula, Montana, USA.
LA  - eng
PT  - Journal Article
DEP - 20210617
PL  - United States
TA  - High Alt Med Biol
JT  - High altitude medicine & biology
JID - 100901183
RN  - 0 (RNA, Messenger)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Adult
MH  - Gene Expression
MH  - *Glycogen
MH  - Humans
MH  - Hypoxia
MH  - Male
MH  - *Muscle, Skeletal
MH  - RNA, Messenger
MH  - Young Adult
OTO - NOTNLM
OT  - HIF-1
OT  - PGC-1alpha
OT  - altitude
OT  - biopsy
OT  - environmental
OT  - hypoxia
OT  - mRNA
EDAT- 2021/06/19 06:00
MHDA- 2021/10/12 06:00
CRDT- 2021/06/18 12:14
PHST- 2021/06/19 06:00 [pubmed]
PHST- 2021/10/12 06:00 [medline]
PHST- 2021/06/18 12:14 [entrez]
AID - 10.1089/ham.2021.0028 [doi]
PST - ppublish
SO  - High Alt Med Biol. 2021 Sep;22(3):300-307. doi: 10.1089/ham.2021.0028. Epub 2021 
      Jun 17.