PMID- 34144067
OWN - NLM
STAT- MEDLINE
DCOM- 20210817
LR  - 20210817
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 185
DP  - 2021 Aug 31
TI  - Molecular characterization, expression analysis and function identification of 
      Pf_TNF-alpha and its two receptors Pf_TNFR1 and Pf_TNFR2 in yellow catfish 
      (Pelteobagrus fulvidraco).
PG  - 176-193
LID - S0141-8130(21)01318-0 [pii]
LID - 10.1016/j.ijbiomac.2021.06.090 [doi]
AB  - Inflammation is a common manifestation of body immunity and mediates a cascade of 
      cytokines. Tumor necrosis factor-alpha (TNF-alpha), as a multi-effect cytokine, plays an 
      important role in the inflammatory response by interacting with its receptor 
      (TNFR). In this study, Pf_TNF-alpha, Pf_TNFR1 and Pf_TNFR2 genes were cloned from 
      yellow catfish (Pelteobagrus fulvidraco), and bioinformatics analyses showed that 
      the three genes were conserved and possessed similar sequence characteristics as 
      those of other vertebrates. The qPCR results showed that Pf_TNF-alpha, Pf_TNFR1 and 
      Pf_TNFR2 mRNAs were constitutively expressed in 14 tissues and the lymphocytes of 
      four tissues from healthy adults. The mRNA expression levels of Pf_TNF-alpha and 
      Pf_TNFR1 genes were significantly up-regulated in the spleen, liver, trunk 
      kidney, head kidney and gill after Edwardsiella ictaluri infection, while the 
      mRNA expression of Pf_TNFR2 was significantly up-regulated in the spleen, and 
      down-regulated in the liver and gill. In the isolated peripheral blood leukocytes 
      (PBLs) of yellow catfish, the expression of Pf_TNF-alpha mRNA was notably 
      up-regulated and the two Pf_TNFR transcripts were distinctly down-regulated after 
      stimulation with lipopolysaccharides (LPS), peptidoglycan (PGN), 
      polyinosinic-polycytidylic acid (Poly I:C) and phytohaemagglutinin (PHA). After 
      stimulated by recombinant (r) Pf_sTNF protein, the mRNA expressions of various 
      inflammatory factors genes were up-regulated in the PBLs. Meanwhile, rPf_sTNF 
      promoted the phagocytic activity of leukocytes, whereas the activity mediated by 
      rPf_sTNF could be inhibited by rPf_TNFR1CRD2/3 and rPf_TNFR2CRD2/3. The 
      up-regulation of TNF-alpha and IL-1beta mRNAs expression triggered by rPf_sTNF could be 
      inhibited by MAPK inhibitor (VX-702) and NF-kappaB inhibitor (PDTC). rPf_sTNF induced 
      the expression of FADD mRNA in PBLs and increased the apoptotic rate of PBLs, and 
      inhibiting the NF-kappaB and MAPK signal pathways could enhance the apoptosis of 
      PBLs. The results indicate that Pf_TNF-alpha, Pf_TNFR1 and Pf_TNFR2 play important 
      roles in the immune response of yellow catfish to bacterial invasion.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Hu, Yuan-Hong
AU  - Hu YH
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Zhou, Xu
AU  - Zhou X
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Jiang, Xin-Xin
AU  - Jiang XX
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Zhang, Gui-Rong
AU  - Zhang GR
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Shi, Ze-Chao
AU  - Shi ZC
AD  - Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture 
      and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of 
      Fishery Sciences, Wuhan 430223, PR China.
FAU - Ji, Wei
AU  - Ji W
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Ma, Xu-Fa
AU  - Ma XF
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China.
FAU - Luo, Zun-Lan
AU  - Luo ZL
AD  - State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese 
      Research Academy of Environmental Sciences, Beijing 100012, PR China. Electronic 
      address: luozl@craes.org.cn.
FAU - Wei, Kai-Jian
AU  - Wei KJ
AD  - National Demonstration Center for Experimental Aquaculture Education, Huazhong 
      Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater 
      Animal Breeding, Ministry of Agriculture and Rural Affairs, College of Fisheries, 
      Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: 
      kjwei@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210616
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Fish Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptidoglycan)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Catfishes/*genetics
MH  - Cloning, Molecular/*drug effects
MH  - Computational Biology
MH  - Female
MH  - Fish Proteins/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Organ Specificity
MH  - Peptidoglycan/pharmacology
MH  - Phylogeny
MH  - Phytohemagglutinins/pharmacology
MH  - Poly I-C/pharmacology
MH  - Receptors, Tumor Necrosis Factor/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
OTO - NOTNLM
OT  - Functional analysis
OT  - Gene expression
OT  - Pelteobagrus fulvidraco
OT  - Pf_TNF-alpha
OT  - Pf_TNFR1
OT  - Pf_TNFR2
EDAT- 2021/06/19 06:00
MHDA- 2021/08/18 06:00
CRDT- 2021/06/18 20:12
PHST- 2021/03/26 00:00 [received]
PHST- 2021/05/18 00:00 [revised]
PHST- 2021/06/12 00:00 [accepted]
PHST- 2021/06/19 06:00 [pubmed]
PHST- 2021/08/18 06:00 [medline]
PHST- 2021/06/18 20:12 [entrez]
AID - S0141-8130(21)01318-0 [pii]
AID - 10.1016/j.ijbiomac.2021.06.090 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 Aug 31;185:176-193. doi: 
      10.1016/j.ijbiomac.2021.06.090. Epub 2021 Jun 16.