PMID- 34147958 OWN - NLM STAT- MEDLINE DCOM- 20211104 LR - 20211104 IS - 1873-1716 (Electronic) IS - 0167-5877 (Linking) VI - 193 DP - 2021 Aug TI - Finding PRRSV in sow herds: Family oral fluids vs. serum samples from due-to-wean pigs. PG - 105397 LID - S0167-5877(21)00141-0 [pii] LID - 10.1016/j.prevetmed.2021.105397 [doi] AB - The aim of this study was to compare the detection of porcine reproductive and respiratory syndrome virus (PRRSV) in due-to-wean litters in commercial swine breeding herds using family oral fluids (FOF) vs. individual piglet serum samples. FOF and piglet serum samples were collected in 199 due-to-wean litters on six farms containing 2177 piglets. All samples were individually tested for PRRSV RNA by RT-rtPCR. A litter was considered PRRSV-positive when PRRSV RNA was detected in >/= 1 piglet serum sample or the FOF sample. Mixed effect logistic regression with farm as a random effect was used 1) to evaluate the probability of obtaining a PRRSV RNA positive FOF as a function of the proportion of viremic piglets in a litter and 2) the effect of litter size and parity on the probability that a litter would test PRRSV RNA positive in FOF. A Bayesian prevalence estimation under misclassification (BayesPEM) analysis was used to calculate the PRRSV prevalence and 95 % credible interval given the condition that all samples (FOF and serum) tested negative. In total, 34 of 199 litters (17.1 %) contained >/= 1 viremic piglet(s), and 28 of 199 litters (14.1 %) were FOF positive. When all piglet serum samples within a litter tested negative, 1 of 165 FOF (0.6 %) tested PRRSV RNA positive. The probability of a PCR-positive FOF sample from litters with 10 %, 20 %, 30 %, 40 %, and 50 % within-litter PRRSV prevalence was 3.5 %, 35.1 %, 88.8 %, 99.2 %, and >99.9 %, respectively. The odds of a PCR-positive FOF in a first parity litter were 3.36 times (95 % CI: 2.10-5.38) that of a parity >/= 2 litter. The odds of a positive FOF result in a litter with 11 piglets. FOF was shown to be an efficacious sample type for PRRSV detection in farrowing rooms. A risk-based approach for litter selection combined with FOF collection can be used to improve on-farm PRRSV detection with a limited sample size, compared to sampling multiple individual pigs. Finally, the BayesPEM analysis showed that PRRSV may still be present in breeding herds when all samples (serum and FOF) test PRRSV RNA negative, i.e., negative surveillance results should be interpreted with caution. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Almeida, M N AU - Almeida MN AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States. Electronic address: malmeida@iastate.edu. FAU - Zhang, M AU - Zhang M AD - Department of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, Iowa, United States. FAU - Zimmerman, J J AU - Zimmerman JJ AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States. FAU - Holtkamp, D J AU - Holtkamp DJ AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States. FAU - Linhares, D C L AU - Linhares DCL AD - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 50011, United States. LA - eng PT - Comparative Study PT - Journal Article DEP - 20210605 PL - Netherlands TA - Prev Vet Med JT - Preventive veterinary medicine JID - 8217463 SB - IM MH - Animals MH - Bayes Theorem MH - Blood/virology MH - Female MH - Litter Size MH - *Porcine Reproductive and Respiratory Syndrome/diagnosis/epidemiology MH - *Porcine respiratory and reproductive syndrome virus/isolation & purification MH - Pregnancy MH - Saliva/virology MH - Swine MH - Weaning OTO - NOTNLM OT - Monitoring OT - Oral fluids OT - PRRSV OT - Surveillance OT - Swine OT - serum EDAT- 2021/06/21 06:00 MHDA- 2021/11/05 06:00 CRDT- 2021/06/20 20:52 PHST- 2020/12/15 00:00 [received] PHST- 2021/04/27 00:00 [revised] PHST- 2021/05/31 00:00 [accepted] PHST- 2021/06/21 06:00 [pubmed] PHST- 2021/11/05 06:00 [medline] PHST- 2021/06/20 20:52 [entrez] AID - S0167-5877(21)00141-0 [pii] AID - 10.1016/j.prevetmed.2021.105397 [doi] PST - ppublish SO - Prev Vet Med. 2021 Aug;193:105397. doi: 10.1016/j.prevetmed.2021.105397. Epub 2021 Jun 5.