PMID- 34148793 OWN - NLM STAT- MEDLINE DCOM- 20211125 LR - 20211125 IS - 1347-4421 (Electronic) IS - 1347-4421 (Linking) VI - 132 IP - 3 DP - 2021 Sep TI - Inhibitory effect of Morus australis leaf extract and its component iminosugars on intestinal carbohydrate-digesting enzymes. PG - 226-233 LID - S1389-1723(21)00141-9 [pii] LID - 10.1016/j.jbiosc.2021.05.005 [doi] AB - alpha-Amylase and alpha-glucosidase are central enzymes involved in the digestion of carbohydrates. alpha-Glucosidase includes maltase-glucoamylase and sucrase-isomaltase. We have previously performed the kinetic analysis of the inhibitory effects of powdered or roasted Morus australis leaf extract and its component iminosugars, such as 1-deoxynojirimycin (1-DNJ), fagomine, and 2-O-alpha-d-galactopyranosyl deoxynojirimycin (GAL-DNJ) on the activity of maltase. In this study, we analyzed the inhibitory effects of the aforementioned compounds against alpha-amylase, glucoamylase, sucrase, and isomaltase. At pH 6.0 and 37 degrees C, each leaf extract sample inhibited glucoamylase, sucrase, and isomaltase but not alpha-amylase. 1-DNJ and fagomine showed weak alpha-amylase inhibitory activity while GAL-DNJ exhibited none. 1-DNJ showed a strong glucoamylase, sucrase, and isomaltase inhibitory potential. The inhibitory potential against these three enzymes was 18-500 and 1500-3000-fold higher in the case of 1-DNJ than that observed in the case of fagomine and GAL-DNJ, respectively. We also observed that the indigestible dextrin could considerably inhibit alpha-amylase. When the powdered M. australis leaf extract was blended with indigestible dextrin, the mixture inhibited alpha-amylase, as well as maltase, glucoamylase, sucrase, and isomaltase. These results suggest that the ingestion of the leaf extract blended with indigestible dextrin might have the potential to efficiently suppress the postprandial blood glucose level increase. CI - Copyright (c) 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. FAU - Qiao, Ying AU - Qiao Y AD - Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. FAU - Ito, Masaaki AU - Ito M AD - Department of Bioresources Engineering, National Institute of Technology, Okinawa College, 905 Henoko, Nago 905-2192, Japan. FAU - Kimura, Toshiyuki AU - Kimura T AD - Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. FAU - Ikeuchi, Takeaki AU - Ikeuchi T AD - Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. FAU - Takita, Teisuke AU - Takita T AD - Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. FAU - Yasukawa, Kiyoshi AU - Yasukawa K AD - Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: yasukawa.kiyoshi.7v@kyoto-u.ac.jp. LA - eng PT - Journal Article DEP - 20210618 PL - Japan TA - J Biosci Bioeng JT - Journal of bioscience and bioengineering JID - 100888800 RN - 0 (Plant Extracts) RN - EC 3.2.1.20 (alpha-Glucosidases) RN - EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase) SB - IM MH - Glucan 1,4-alpha-Glucosidase/metabolism MH - Kinetics MH - *Morus MH - Plant Extracts/pharmacology MH - alpha-Glucosidases/metabolism OTO - NOTNLM OT - Iminosugar OT - Indigestible dextrin OT - Morus australis OT - alpha-Amylase OT - alpha-Glucosidase EDAT- 2021/06/22 06:00 MHDA- 2021/11/26 06:00 CRDT- 2021/06/21 05:36 PHST- 2021/04/01 00:00 [received] PHST- 2021/05/13 00:00 [revised] PHST- 2021/05/14 00:00 [accepted] PHST- 2021/06/22 06:00 [pubmed] PHST- 2021/11/26 06:00 [medline] PHST- 2021/06/21 05:36 [entrez] AID - S1389-1723(21)00141-9 [pii] AID - 10.1016/j.jbiosc.2021.05.005 [doi] PST - ppublish SO - J Biosci Bioeng. 2021 Sep;132(3):226-233. doi: 10.1016/j.jbiosc.2021.05.005. Epub 2021 Jun 18.