PMID- 34149994
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240402
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 5
DP  - 2021
TI  - A treatment combined prussian blue nanoparticles with low-intensity pulsed 
      ultrasound alleviates cartilage damage in knee osteoarthritis by initiating 
      PI3K/Akt/mTOR pathway.
PG  - 3987-4006
AB  - Reactive oxidative stress (ROS) related apoptosis in chondrocytes and 
      extracellular matrix (ECM) degradation play crucial roles in the process of 
      osteoarthritis. Prussian blue nanoparticles are known to scavenge ROS in 
      cellular. Low-intensity pulsed ultrasound has been used as a non-invasive 
      modality for the is widely used in clinical rehabilitation management of OA. In 
      this study, we aim to investigate the effects of PBNPs/LIPUS combined treatment 
      on knee osteoarthritis (KOA) and to determine whether phosphoinositide 3-kinase 
      (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway mediates this 
      process. Use LPS to process primary cells of knee joint cartilage to establish a 
      cartilage knee arthritis model. After treated with LIPUS and PBNPs, cell 
      viability was rated by CCK-8 and ROS levels were assessed by DCFH-DA. Articular 
      pathological changes were observed by naked eyes, H&E, and Safranin O staining, 
      then monitored by cartilage lesion grades and Mankin's score. Cellular ROS, 
      apoptosis rate, and TUNEL staining of chondrocytes were fairly decreased in the 
      PBNPs group and the LIPUS group but drastically down-regulated in the PBNPs/LIPUS 
      combination treatment group when compared with the LPS group. Western blot 
      results showed that the cleaved caspase-3, Bax, IL-1beta, MMP3 and MMP13 in the 
      PBNPs and LIPUS groups slightly decreased, and Bcl2 increased slightly, while in 
      the combination treatment group, the former was significantly decreased, and Bcl2 
      was Significantly increased. The PBNPs/LIPUS combination treatment reduced 
      cellular ROS, apoptosis, and matrix metalloproteinases (MMPs), as a consequence, 
      alleviated articular cartilage damage in KOA. Moreover, the PBNPs/LIPUS 
      combination treatment suppressed the JNK/c-Jun signal pathway.
CI  - AJTR Copyright (c) 2021.
FAU - Zuo, Deyu
AU  - Zuo D
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
FAU - Tan, Botao
AU  - Tan B
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
FAU - Jia, Gongwei
AU  - Jia G
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
FAU - Wu, Dandong
AU  - Wu D
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
FAU - Yu, Lehua
AU  - Yu L
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
FAU - Jia, Lang
AU  - Jia L
AD  - Department of Rehabilitation Medicine, The Second Affiliated Hospital of 
      Chongqing Medical University People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210515
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8205753
OTO - NOTNLM
OT  - Osteoarthritis (OA)
OT  - low-intensity pulsed ultrasound (LIPUS)
OT  - prussian blue nanoparticles (PBNPs)
COIS- None.
EDAT- 2021/06/22 06:00
MHDA- 2021/06/22 06:01
PMCR- 2021/05/15
CRDT- 2021/06/21 05:55
PHST- 2020/09/29 00:00 [received]
PHST- 2021/03/02 00:00 [accepted]
PHST- 2021/06/21 05:55 [entrez]
PHST- 2021/06/22 06:00 [pubmed]
PHST- 2021/06/22 06:01 [medline]
PHST- 2021/05/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 May 15;13(5):3987-4006. eCollection 2021.