PMID- 34150003
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210622
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 5
DP  - 2021
TI  - Circular RNA circ_0084927 regulates proliferation, apoptosis, and invasion of 
      breast cancer cells via miR-142-3p/ERC1 pathway.
PG  - 4120-4136
AB  - OBJECTIVE: We aimed to investigate the mechanism of circular RNA circ_0084927 in 
      the progression of breast cancer (BC). METHODS: The levels of circ_0084927, 
      miR-142-3p, and ELKS/RAB6-interacting/CAST family member-1 (ERC1) mRNA in the BC 
      tissues and cells were detected by qRT-PCR. CCK8, colony formation, Transwell, 
      and flow cytometry assays were performed to examine the cell proliferation, 
      colony formation, cell invasion, and apoptosis, respectively, in the BC cells 
      with regulated expressions of circ_0084927, miR-142-3p, and ERC1. RNase R 
      treatment was employed to verify the circular structure of circ_0084927. 
      Nucleocytoplasmic separation experiment, bioinformatics analysis, dual-luciferase 
      reporter assay, and RNA immunoprecipitation were performed to investigate the 
      ceRNA mechanism of circ_0084927. RESULTS: High levels of circ_0084927 and ERC1 
      and low levels of miR-142-3p were detected in the BC tissues and cells. Knockdown 
      of circ_0084927 promoted apoptosis and inhibited proliferation, colony formation, 
      and invasion of BC cells (all P<0.05), whereas overexpression of circ_0084927 in 
      the BC cells achieved the opposite effects. miR-142-3p is the target of 
      circ_0084927. Overexpression of miR-142-3p could inhibit BC cell proliferation, 
      colony formation, and cell invasion and induce apoptosis of the BC cells (all 
      P<0.05), and the effects of miR-142-3p knockout on the BC cells could be reversed 
      by silencing circ_0084927. miR-142-3p could target ERC1. Both ERC1 silencing and 
      circ_0084927 knockout in the BC cells could achieve the tumor-suppressing effect, 
      and this effect could be more remarkable under simultaneous ERC1 silencing and 
      circ_0084927 knockout (all P<0.05). CONCLUSION: Circ_0084927 can promote the 
      progression of BC by regulating the miR-142-3p/ERC1 axis.
CI  - AJTR Copyright (c) 2021.
FAU - Gong, Guohua
AU  - Gong G
AD  - Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for 
      Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, 
      China.
AD  - First Clinical Medical of Inner Mongolia University for Nationalities Tongliao, 
      Inner Mongolia Autonomous Region, China.
FAU - She, Jikai
AU  - She J
AD  - Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for 
      Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, 
      China.
AD  - Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for The 
      Nationalities Tongliao, Inner Mongolia Autonomous Region, China.
FAU - Fu, Danni
AU  - Fu D
AD  - Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for 
      Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, 
      China.
AD  - Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for The 
      Nationalities Tongliao, Inner Mongolia Autonomous Region, China.
FAU - Zhen, Dong
AU  - Zhen D
AD  - Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for 
      Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, 
      China.
AD  - Medicinal Chemistry and Pharmacology Institute, Inner Mongolia University for The 
      Nationalities Tongliao, Inner Mongolia Autonomous Region, China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - Inner Mongolia Key Laboratory of Mongolian Medicine Pharmacology for 
      Cardio-Cerebral Vascular System Tongliao, Inner Mongolia Autonomous Region, 
      China.
AD  - First Clinical Medical of Inner Mongolia University for Nationalities Tongliao, 
      Inner Mongolia Autonomous Region, China.
LA  - eng
PT  - Journal Article
DEP - 20210515
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8205726
OTO - NOTNLM
OT  - ELKS/RAB6-interacting/CAST family member-1
OT  - breast cancer
OT  - circ_0084927
OT  - miR-142-3p
COIS- None.
EDAT- 2021/06/22 06:00
MHDA- 2021/06/22 06:01
PMCR- 2021/05/15
CRDT- 2021/06/21 05:55
PHST- 2020/11/30 00:00 [received]
PHST- 2021/02/09 00:00 [accepted]
PHST- 2021/06/21 05:55 [entrez]
PHST- 2021/06/22 06:00 [pubmed]
PHST- 2021/06/22 06:01 [medline]
PHST- 2021/05/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 May 15;13(5):4120-4136. eCollection 2021.