PMID- 34150927
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220521
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 11
IP  - 10
DP  - 2021 May 20
TI  - In vitro and In vivo CD8(+) T Cell Suppression Assays.
PG  - e4020
LID - 10.21769/BioProtoc.4020 [doi]
LID - e4020
AB  - CD8(+)CD28(-) T suppressor cells (Ts) have been documented to promote immune 
      tolerance by suppressing effector T cell responses to alloantigens following 
      transplantation. The suppressive function of T cells has been defined as the 
      inhibitory effect of Ts on the proliferation rate of effector T cells. 
      (3)H-thymidine is a classical immunological technique for assaying T cell 
      proliferation but this approach has drawbacks such as the inconvenience of 
      working with radioactive materials. Labeling T cells with CFSE allows relatively 
      easy tracking of generations of proliferated cells. In this report, we utilized 
      antigen presenting cells (APCs) and T cells matched for human leukocyte antigen 
      (HLA) class I or class II to study CD8(+)CD28(-) T cell suppression generated in 
      vitro by this novel approach of combining allogeneic APCs and gammac cytokines. The 
      expanded CD8(+)CD28(-) T cells were isolated (purity 95%) and evaluated for their 
      suppressive capacity in mixed lymphocyte reactions using CD4(+) T cells as 
      responders. Here, we present our adapted protocol for assaying the Ts 
      allospecific suppression of CFSE-labeled responder T cells.
CI  - Copyright (c) 2021 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Xie, Lu
AU  - Xie L
AD  - Department of Urology, Guangdong Provincial People's Hospital (Guangdong Academy 
      of Medical Sciences), Guangzhou, China.
AD  - School of Medicine, South China University of Technology, Guangzhou, China.
FAU - Liu, Guihuan
AU  - Liu G
AD  - Department of Immunology, School of Basic Medical Science, Southern Medical 
      University, Guangzhou, China.
FAU - Liu, Yanjun
AU  - Liu Y
AD  - Department of Immunology, School of Basic Medical Science, Southern Medical 
      University, Guangzhou, China.
FAU - Yu, Yuming
AU  - Yu Y
AD  - Department of Urology, Guangdong Provincial People's Hospital (Guangdong Academy 
      of Medical Sciences), Guangzhou, China.
AD  - School of Medicine, South China University of Technology, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20210520
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC8187121
OTO - NOTNLM
OT  - CD8+CD28- T cells
OT  - Alloantigen specific tolerance
OT  - Common gamma chain cytokines
OT  - Immune tolerance
OT  - Suppression
COIS- Competing interestsThe authors declare no competing interests, financial or 
      non-financial.
EDAT- 2021/06/22 06:00
MHDA- 2021/06/22 06:01
PMCR- 2022/05/20
CRDT- 2021/06/21 06:02
PHST- 2020/09/24 00:00 [received]
PHST- 2021/03/03 00:00 [revised]
PHST- 2021/03/04 00:00 [accepted]
PHST- 2021/06/21 06:02 [entrez]
PHST- 2021/06/22 06:00 [pubmed]
PHST- 2021/06/22 06:01 [medline]
PHST- 2022/05/20 00:00 [pmc-release]
AID - e4020 [pii]
AID - 4020 [pii]
AID - 10.21769/BioProtoc.4020 [doi]
PST - epublish
SO  - Bio Protoc. 2021 May 20;11(10):e4020. doi: 10.21769/BioProtoc.4020. eCollection 
      2021 May 20.