PMID- 34154318
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20220531
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 56
IP  - 6
DP  - 2021 Jun 25
TI  - [Expression of lncRNA MIR210HG in preeclampsia placental tissue and its 
      functional analysis].
PG  - 425-433
LID - 10.3760/cma.j.cn112141-20210118-00029 [doi]
AB  - Objective: To investigate the differential expression of long non-coding RNA 
      (lncRNA) in placental tissues of women with preeclampsia (PE) and the effect of 
      MIR210HG on the biological function of HTR8/SVneo cells. Methods: A total of 39 
      cases of PE women (PE group) and 39 cases of normal pregnant women (CTL group) 
      admitted to the Affiliated Hospital of Qingdao University from July 2018 to July 
      2019 were collected. (1) Transcriptome sequencing (RNA-seq) was used to analyze 
      the differentially expressed lncRNAs in the placental tissues of the two groups. 
      (2) The expression level of MIR210HG, one of the differentially expressed 
      lncRNAs, in the placental tissues of the two groups was detected by real-time 
      quantitative PCR. And the correlations between the expression level of MIR210HG 
      and systolic blood pressure, diastolic blood pressure and neonatal birth weight 
      were analyzed. (3) The constructed small interfering RNA and negative control 
      (NC) RNA were transfected into the HTR8/SVneo cells. The cells were divided into 
      MIR210HG knockdown (KD) group and NC group. The effects of living cell counting 
      (CCK-8) and transwell assay on the proliferation and migration of HTR8/SVneo 
      cells were detected. (4) RNA interacting with MIR210HG was predicted using the 
      Encyclopedia of RNA Interactomes (ENCORI) database. Gene Ontology (GO) functional 
      annotation, Kyoto Encyclopedia of Gene and Genomes (KEGG) and BioCarta pathway 
      enrichment analysis were performed. Results: (1) A total of 26 significantly 
      differentially expressed lncRNAs were found by RNA-seq, among which 21 lncRNAs 
      were up-regulated and 5 lncRNAs were down-regulated. (2) The relative expression 
      level of MIR210HG in the PE group was significantly higher than that in the CTL 
      group (9.30+/-1.90 and 1.10+/-0.20, respectively; t=4.425, P<0.01). The relative 
      expression level of MIR210HG had positive linear correlation with systolic blood 
      pressure (r(2)=0.234, P<0.05) and diastolic blood pressure (r(2)=0.190, P<0.05), 
      but had a negative linear correlation with newborn birth weight (r(2)=0.157, 
      P<0.05). (3) Compared with the NC group, the proliferation and migration ability 
      of HTR8/SVneo cells in the KD group were increased (all P<0.05). (4) A total of 
      38 RNAs that might interact with MIR210HG were predicted by ENCORI database. GO 
      functional annotation analysis showed that MIR210HG might be involved in the 
      functions of 27 pathways, including the regulation of production of molecular 
      mediator of immune response, etc; KEGG pathway analysis showed that MIR210HG 
      might be involved in the function of 8 pathways including allograft rejection, 
      etc; Biocarta pathway analysis showed that MIR210HG may be involved in the 
      functions of 8 pathways, including the eukaryotic initiation factor (eIF) 
      pathway, etc. Conclusion: The expression of MIR210HG is up-regulated in the 
      placental tissue of PE women, and MIR210HG might be a regulator of the biological 
      behavior of trophoblast cells.
FAU - Li, X L
AU  - Li XL
AD  - Department of Endocrinology and Metabolism, Medical College of Qingdao 
      University, Qingdao 266003, China.
FAU - Zhang, L
AU  - Zhang L
AD  - Prenatal Diagnosis Center and Medical Genetic Department, the Affiliated Hospital 
      of Qingdao University, Qingdao 266003, China.
FAU - Hou, B
AU  - Hou B
AD  - Department of Cardiology, the Affiliated Hospital of Qingdao University, Qingdao 
      266003, China.
FAU - Piao, S F
AU  - Piao SF
AD  - Department of Obstetrics, the Affiliated Hospital of Qingdao University, Qingdao 
      266003, China.
FAU - Tang, Q
AU  - Tang Q
AD  - Prenatal Diagnosis Center and Medical Genetic Department, the Affiliated Hospital 
      of Qingdao University, Qingdao 266003, China.
FAU - Dong, M
AU  - Dong M
AD  - Qingdao International Travel Health Care Center, Qingdao 266071, China.
FAU - Liu, S G
AU  - Liu SG
AD  - Prenatal Diagnosis Center and Medical Genetic Department, the Affiliated Hospital 
      of Qingdao University, Qingdao 266003, China.
FAU - Cao, C X
AU  - Cao CX
AD  - Department of Geriatrics, the Affiliated Hospital of Qingdao University, Qingdao 
      266003, China.
LA  - chi
GR  - 82071667/National Natural Science Foundation of China/
GR  - ZR2019MH127/Natural Science Foundation of Shandong Province/
GR  - 2019GSF108106/Shandong Province Key Research and Development Plan/
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Female
MH  - Humans
MH  - Infant, Newborn
MH  - *Placenta
MH  - *Pre-Eclampsia/genetics
MH  - Pregnancy
MH  - *RNA, Long Noncoding/genetics
MH  - RNA, Small Interfering
MH  - Trophoblasts
EDAT- 2021/06/23 06:00
MHDA- 2021/06/24 06:00
CRDT- 2021/06/22 03:54
PHST- 2021/06/22 03:54 [entrez]
PHST- 2021/06/23 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
AID - 10.3760/cma.j.cn112141-20210118-00029 [doi]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2021 Jun 25;56(6):425-433. doi: 
      10.3760/cma.j.cn112141-20210118-00029.