PMID- 34155336
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20240404
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 15
IP  - 12
DP  - 2021 Dec
TI  - "Candidatus Dechloromonas phosphoritropha" and "Ca. D. phosphorivorans", novel 
      polyphosphate accumulating organisms abundant in wastewater treatment systems.
PG  - 3605-3614
LID - 10.1038/s41396-021-01029-2 [doi]
AB  - Members of the genus Dechloromonas are often abundant in enhanced biological 
      phosphorus removal (EBPR) systems and are recognized putative polyphosphate 
      accumulating organisms (PAOs), but their role in phosphate removal is still 
      unclear. Here, we used 16S rRNA gene sequencing and fluorescence in situ 
      hybridization (FISH) to investigate the abundance and distribution of 
      Dechloromonas spp. in Danish and global wastewater treatment plants. The two most 
      abundant species worldwide revealed in situ dynamics of important intracellular 
      storage polymers, measured by FISH-Raman in activated sludge from four full-scale 
      EBPR plants and from a lab-scale reactor fed with different substrates. Moreover, 
      seven distinct Dechloromonas species were determined from a set of ten 
      high-quality metagenome-assembled genomes (MAGs) from Danish EBPR plants, each 
      encoding the potential for polyphosphate (poly-P), glycogen, and 
      polyhydroxyalkanoates (PHA) accumulation. The two species exhibited an in situ 
      phenotype in complete accordance with the metabolic information retrieved by the 
      MAGs, with dynamic levels of poly-P, glycogen, and PHA during feast-famine 
      anaerobic-aerobic cycling, legitimately placing these microorganisms among the 
      important PAOs. They are potentially involved in denitrification showing niche 
      partitioning within the genus and with other important PAOs. As no isolates are 
      available for the two species, we propose the names Candidatus Dechloromonas 
      phosphoritropha and Candidatus Dechloromonas phosphorivorans.
CI  - (c) 2021. The Author(s).
FAU - Petriglieri, Francesca
AU  - Petriglieri F
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark.
FAU - Singleton, Caitlin
AU  - Singleton C
AUID- ORCID: 0000-0001-9688-8208
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark.
FAU - Peces, Miriam
AU  - Peces M
AUID- ORCID: 0000-0003-2522-7490
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark.
FAU - Petersen, Jette F
AU  - Petersen JF
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark.
FAU - Nierychlo, Marta
AU  - Nierychlo M
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark.
FAU - Nielsen, Per H
AU  - Nielsen PH
AUID- ORCID: 0000-0002-6402-1877
AD  - Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg 
      University, Aalborg, Denmark. phn@bio.aau.dk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210621
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (Polyphosphates)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - Bioreactors
MH  - In Situ Hybridization, Fluorescence
MH  - Phosphorus
MH  - *Polyphosphates
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage
MH  - *Water Purification
PMC - PMC8630035
COIS- The authors declare no competing interests.
EDAT- 2021/06/23 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/06/21
CRDT- 2021/06/22 06:27
PHST- 2020/12/04 00:00 [received]
PHST- 2021/06/02 00:00 [accepted]
PHST- 2021/05/22 00:00 [revised]
PHST- 2021/06/23 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/06/22 06:27 [entrez]
PHST- 2021/06/21 00:00 [pmc-release]
AID - 10.1038/s41396-021-01029-2 [pii]
AID - 1029 [pii]
AID - 10.1038/s41396-021-01029-2 [doi]
PST - ppublish
SO  - ISME J. 2021 Dec;15(12):3605-3614. doi: 10.1038/s41396-021-01029-2. Epub 2021 Jun 
      21.