PMID- 34155704
OWN - NLM
STAT- MEDLINE
DCOM- 20210927
LR  - 20210927
IS  - 1099-1573 (Electronic)
IS  - 0951-418X (Linking)
VI  - 35
IP  - 7
DP  - 2021 Jul
TI  - Anti-gastric cancer activity and mechanism of natural compound "Heilaohulignan C" 
      isolated from Kadsura coccinea.
PG  - 3977-3987
LID - 10.1002/ptr.7114 [doi]
AB  - In this research, we analyzed the antitumor activity of one new compound 
      Heilaohulignan C (B-6) on the human gastric carcinoma cells. MTT, cell migration, 
      Calcein AM/Propidium Iodide (PI), and flow cytometry in BGC-823 cell line 
      (gastric tumor). Western blot was utilized to distinguish the protein level. 
      Xenografts nude mice were used for in vivo anticancer analysis. H&E staining and 
      laboratory investigation was accomplished for toxicity study. MTT test 
      demonstrated the cytotoxicity of BGC-823 cells, Calcein AM/Propidium Iodide (PI) 
      examine indicated increment dead cells proportion with a high dose of B-6, Flow 
      cytometry (FACS) measure showed that B-6 influenced gastric cancer cells by 
      initiating apoptosis. Western blot analysis confirmed that (B-6) decrease the 
      level of Bcl-2 and increase the level of p53, Bax, and cleaved Caspase-3, this 
      confirms that the B-6 doing the apoptosis through caspase and cytochrome C 
      apoptotic pathways. Also, B-6 particularly decline the tumor volume and tumor 
      size in the xenograft mice. H&E staining additionally supports that B-6 does not 
      have any toxic impact on the normal tissues. This research supports that B-6 have 
      pharmacological activity against gastric cancer, by p53 and mitochondrial 
      dependent apoptotic pathway, and have no toxicity on normal tissues.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Daniyal, Muhammad
AU  - Daniyal M
AUID- ORCID: 0000-0002-5663-5211
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Liu, Yongbei
AU  - Liu Y
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Yang, Yupei
AU  - Yang Y
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Xiao, Feng
AU  - Xiao F
AD  - College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics 
      and Developmental Regulation, Hunan University, Changsha, Hunan, China.
FAU - Fan, Jialong
AU  - Fan J
AD  - College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics 
      and Developmental Regulation, Hunan University, Changsha, Hunan, China.
FAU - Yu, Huanghe
AU  - Yu H
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Qiu, Yixing
AU  - Qiu Y
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Liu, Bin
AU  - Liu B
AD  - College of Biology, Hunan Province Key Laboratory of Plant Functional Genomics 
      and Developmental Regulation, Hunan University, Changsha, Hunan, China.
FAU - Wang, Wei
AU  - Wang W
AUID- ORCID: 0000-0003-0876-2205
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
FAU - Yuhui, Qin
AU  - Yuhui Q
AD  - TCM and Ethnomedicine Innovative & Development International Laboratory, 
      Academician Atta-ur-Rahman Belt and Road Traditional Medicine Research Center, 
      School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China.
LA  - eng
GR  - 2018SK2110/Hunan Department of Science and Technology/
GR  - 2018Wk2081/Hunan Department of Science and Technology/
GR  - 2018FY100703/Ministry of Science and Technology/
GR  - 2018YFC1707902/Ministry of Science and Technology/
GR  - 81874369/Natural Science Foundation of China/
GR  - 81673579/Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210621
PL  - England
TA  - Phytother Res
JT  - Phytotherapy research : PTR
JID - 8904486
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Humans
MH  - *Kadsura/chemistry
MH  - Mice
MH  - Mice, Nude
MH  - *Stomach Neoplasms/drug therapy
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - Heilaohulignan C
OT  - apoptosis
OT  - gastric cancer
OT  - natural compound
OT  - p53
EDAT- 2021/06/23 06:00
MHDA- 2021/09/28 06:00
CRDT- 2021/06/22 06:50
PHST- 2021/02/20 00:00 [revised]
PHST- 2020/12/18 00:00 [received]
PHST- 2021/03/25 00:00 [accepted]
PHST- 2021/06/23 06:00 [pubmed]
PHST- 2021/09/28 06:00 [medline]
PHST- 2021/06/22 06:50 [entrez]
AID - 10.1002/ptr.7114 [doi]
PST - ppublish
SO  - Phytother Res. 2021 Jul;35(7):3977-3987. doi: 10.1002/ptr.7114. Epub 2021 Jun 21.