PMID- 34156041
OWN - NLM
STAT- MEDLINE
DCOM- 20220119
LR  - 20221207
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 12
IP  - 15
DP  - 2021 Aug 2
TI  - Biogenic selenium nanoparticles by Lactobacillus casei ATCC 393 alleviate the 
      intestinal permeability, mitochondrial dysfunction and mitophagy induced by 
      oxidative stress.
PG  - 7068-7080
LID - 10.1039/d0fo03141k [doi]
AB  - Selenium (Se) is an essential trace element. Nano-selenium has attracted great 
      attention due to its various biological properties, especially strong antioxidant 
      activity, high bioavailability, and low toxicity. Our previous studies 
      demonstrated that the selenium nanoparticles (SeNPs) synthesized by Lactobacillus 
      casei ATCC 393 (L. casei ATCC 393) alleviate hydrogen peroxide (H2O2)-induced 
      intestinal epithelial barrier dysfunction via the mitochondrial pathway. However, 
      the mechanism of SeNPs exerting antioxidant activity through the mitochondrial 
      pathway remains unclear. This study was conducted to investigate the role of 
      mitophagy in the protective effects of SeNPs on H2O2-induced porcine intestinal 
      epithelial cells against oxidative damage. The results showed that the SeNPs 
      synthesized by L. casei ATCC 393 had no cytotoxicity on IPEC-J2 cells and 
      effectively antagonized the cytotoxicity of 500 muM H2O2 on IPEC-J2 cells. 
      Moreover, SeNPs attenuated the H2O2-induced intestinal epithelial barrier 
      dysfunction and ROS overproduction, as well as alleviated the adenosine 
      triphosphate (ATP) level and the mitochondrial membrane potential (MMP) decrease. 
      In addition, compared to the oxidative stress model group, pretreatment with 
      biogenic SeNPs significantly up-regulated the expression levels of occludin and 
      claudin-1. Moreover, when compared to the oxidative stress model group, SeNPs 
      inhibited the phosphorylation level of the mammalian target of rapamycin (m-TOR), 
      as well as the expression levels of Unc-51-like kinase 1(ULK1), light chain 3 
      (LC3)-II/LC3-I, PTEN-induced kinase 1 (PINK1) and Parkin proteins. The 
      fluorescence colocalization images of mitochondria and lysosomes demonstrated 
      that SeNPs significantly reduced the fusion of mitochondria and lysosomes when 
      compared to the oxidative stress model group. These results demonstrate that the 
      SeNPs synthesized by L. casei ATCC 393 can effectively alleviate the H2O2-induced 
      intestinal epithelial barrier dysfunction through regulating mTOR/PINK1-mediated 
      mitophagy.
FAU - Yan, Shuqi
AU  - Yan S
AD  - The Key Laboratory for Space Bioscience and Biotechnology, School of Life 
      Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China. 
      clxu@nwpu.edu.cn.
FAU - Qiao, Lei
AU  - Qiao L
FAU - Dou, Xina
AU  - Dou X
FAU - Song, Xiaofan
AU  - Song X
FAU - Chen, Yue
AU  - Chen Y
FAU - Zhang, Baohua
AU  - Zhang B
FAU - Xu, Chunlan
AU  - Xu C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - H6241UJ22B (Selenium)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Intestinal Absorption/*drug effects
MH  - Lacticaseibacillus casei/*metabolism
MH  - Metal Nanoparticles/*chemistry
MH  - Mitochondria/drug effects/pathology
MH  - Mitophagy/drug effects
MH  - Oxidative Stress/*drug effects
MH  - *Selenium/chemistry/pharmacology
MH  - Swine
EDAT- 2021/06/23 06:00
MHDA- 2022/01/20 06:00
CRDT- 2021/06/22 08:57
PHST- 2021/06/23 06:00 [pubmed]
PHST- 2022/01/20 06:00 [medline]
PHST- 2021/06/22 08:57 [entrez]
AID - 10.1039/d0fo03141k [doi]
PST - ppublish
SO  - Food Funct. 2021 Aug 2;12(15):7068-7080. doi: 10.1039/d0fo03141k.