PMID- 34157688
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 16
IP  - 5
DP  - 2021 Jul 6
TI  - Effects of bovine cancellous bone powder/poly amino acid composites on cellular 
      behaviors and osteogenic performances.
LID - 10.1088/1748-605X/ac0d94 [doi]
AB  - Xenogeneic bone has good biological activity, but eliminating immunogenicity, 
      while retaining osteogenic abilities, is a challenge. By combining xenogeneic 
      bone with poly amino acid (PAA) that has an amide bond structure, a new type of 
      composite conforming to bionics and low immunogenicity may be obtained. In this 
      study, according to the principles of component bionics, three composites of 
      delipidized cancellous bone powder (DCBP) and PAA were designed and obtained by 
      anin situpolycondensation method, an extrusion molding (EM) method, and a 
      solution-blend method. The three composites were all macroscopically uniform, 
      non-cytotoxic, and demonstrated low immunogenicity by effective removal of 
      residual antigens during preparation. Compared with PAA, mouse bone marrow 
      mesenchymal stem cells (BMSCs) on the surfaces of three composites showed 
      different cellular morphologies. The effects of different preparation methods and 
      cellular morphology on cellular differentiation were confirmed by alkaline 
      phosphatase activity, calcium nodule formation and the expression levels of 
      osteogenic differentiation-related genes (bone morphogenetic protein 2, 
      runt-related transcription factor 2, osteopontin and osteocalcin). Among these 
      composites, DCBP/PAA EM showed best cell proliferation and osteogenic 
      differentiationin vitro, and possessed greater bone formation than PAA in a 
      rabbit femoral condyle study. This study may provide a new method for preparing 
      bioactive bone repair materials with low immunogenicity and superior ability to 
      stimulate differentiation of BMSCsin vitroand osteogenesisin vivo. DCBP/PAA EM 
      might be a promising bone repair material for bone defect treatment.
CI  - (c) 2021 IOP Publishing Ltd.
FAU - Luo, Lin
AU  - Luo L
AD  - College of Physics, Sichuan University, Chengdu, People's Republic of China.
FAU - Li, Pengcheng
AU  - Li P
AD  - College of Physics, Sichuan University, Chengdu, People's Republic of China.
FAU - Ren, Haohao
AU  - Ren H
AD  - College of Physics, Sichuan University, Chengdu, People's Republic of China.
FAU - Ding, Zhengwen
AU  - Ding Z
AD  - College of Physics, Sichuan University, Chengdu, People's Republic of China.
FAU - Yan, Yonggang
AU  - Yan Y
AUID- ORCID: 0000-0002-9534-1013
AD  - College of Physics, Sichuan University, Chengdu, People's Republic of China.
FAU - Li, Shuyang
AU  - Li S
AD  - Sichuan Provincial Laboratory of Orthopaedic Engineering, The Affiliated Hospital 
      of Southwest Medical University, Luzhou, People's Republic of China.
FAU - Yin, Jie
AU  - Yin J
AD  - School of Automation and Information Engineering, Sichuan University of Science 
      and Engineering, Zigong, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210706
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Amino Acids)
RN  - 0 (Bone Substitutes)
SB  - IM
MH  - *Amino Acids/chemistry/pharmacology
MH  - Animals
MH  - Bone Regeneration/drug effects
MH  - *Bone Substitutes/chemistry/pharmacology
MH  - Cancellous Bone/*chemistry
MH  - Cattle
MH  - Cell Differentiation/drug effects
MH  - Female
MH  - Mesenchymal Stem Cells/*drug effects
MH  - Mice
MH  - Osteogenesis/*drug effects
MH  - Rabbits
OTO - NOTNLM
OT  - bone regeneration
OT  - bovine cancellous bone
OT  - cellular and cytoskeletal morphology
OT  - cellular differentiation
OT  - low immunogenicity
EDAT- 2021/06/23 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/06/22 20:25
PHST- 2021/02/05 00:00 [received]
PHST- 2021/06/22 00:00 [accepted]
PHST- 2021/06/23 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/06/22 20:25 [entrez]
AID - 10.1088/1748-605X/ac0d94 [doi]
PST - epublish
SO  - Biomed Mater. 2021 Jul 6;16(5). doi: 10.1088/1748-605X/ac0d94.