PMID- 34159362
OWN - NLM
STAT- MEDLINE
DCOM- 20220103
LR  - 20220103
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 105
IP  - 4
DP  - 2021 Oct 11
TI  - Evidence for a shift in placental HLA-G allelic dominance and the HLA-G isoform 
      profile during a healthy pregnancy and preeclampsiadagger.
PG  - 846-858
LID - 10.1093/biolre/ioab121 [doi]
AB  - Human leukocyte antigen (HLA)-G, which belongs to a nonclassical class Ib major 
      histocompatibility complex gene family expressed by placental trophoblast cells, 
      plays a central role in establishing tolerance to the semiallogeneic fetus and in 
      placentation. HLA-G exists in different soluble or membrane-bound isoforms. 
      Preeclampsia, a major cause of fetal and maternal morbidity and mortality, has 
      been linked to insufficient placentation and an altered immune response in 
      pregnancy, including altered HLA-G expression. The 14 bp insertion/deletion 
      polymorphism in the 3' untranslated region of the gene and the isoform profile 
      may affect HLA-G expression. The aim of the current pilot study was to 
      characterize the expression patterns of HLAG mRNA, protein, and isoform profile 
      in uncomplicated term pregnancies and in cases of preeclampsia. Maternal sHLA-G 
      mRNA and protein levels were slightly reduced in preeclampsia. No difference was 
      found for placental blood, and no correlation between peripheral and placental 
      sHLA-G levels was found. We observed no association between neither fetal nor 
      maternal HLA-G 14 bp insertion/deletion genotypes and preeclampsia, nor a 
      significant difference in isoform profiles. However, in HLA-G 14 bp 
      insertion/deletion heterozygous placental samples, we observed abundant HLA-G1 
      14 bp insertion allele expression in the term placentae, which is contrary to 
      previous findings in first trimester trophoblast. Increased HLA-G1 14 bp 
      insertion allele expression in the placenta was associated with reduced levels of 
      placental sHLA-G and an altered isoform profile with increased relative levels of 
      HLA-G1 and -G5 and reduced levels of HLA-G3. The results indicate that an allelic 
      shift in heterozygous individuals could represent a novel regulatory pathway.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Persson, Gry
AU  - Persson G
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
AD  - Department of Clinical Medicine, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
AD  - The ReproHealth Research Consortium ZUH, Zealand University Hospital, Roskilde, 
      Denmark.
FAU - Staehr, Christina Seefeldt
AU  - Staehr CS
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
AD  - Department of Clinical Medicine, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
AD  - The ReproHealth Research Consortium ZUH, Zealand University Hospital, Roskilde, 
      Denmark.
FAU - Klok, Freja Syrach
AU  - Klok FS
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
AD  - Department of Clinical Medicine, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
AD  - The ReproHealth Research Consortium ZUH, Zealand University Hospital, Roskilde, 
      Denmark.
FAU - Lebech, Morten
AU  - Lebech M
AD  - Department of Clinical Medicine, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
AD  - The ReproHealth Research Consortium ZUH, Zealand University Hospital, Roskilde, 
      Denmark.
AD  - Department of Gynaecology and Obstetrics, Zealand University Hospital, Roskilde, 
      Denmark.
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TVF
AD  - Centre for Immune Regulation and Reproductive Immunology (CIRRI), Department of 
      Clinical Biochemistry, Zealand University Hospital, Roskilde, Denmark.
AD  - Department of Clinical Medicine, Faculty of Health and Medical Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
AD  - The ReproHealth Research Consortium ZUH, Zealand University Hospital, Roskilde, 
      Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (HLA-G Antigens)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Adult
MH  - Female
MH  - Gene Expression Profiling
MH  - HLA-G Antigens/*genetics/metabolism
MH  - Humans
MH  - Pilot Projects
MH  - *Polymorphism, Genetic
MH  - Pre-Eclampsia/*genetics/metabolism
MH  - Pregnancy/*metabolism
MH  - Protein Isoforms
OTO - NOTNLM
OT  - HLA-G
OT  - allele
OT  - isoform
OT  - preeclampsia
OT  - pregnancy
OT  - reproductive immunology
EDAT- 2021/06/24 06:00
MHDA- 2022/01/04 06:00
CRDT- 2021/06/23 07:25
PHST- 2021/03/17 00:00 [received]
PHST- 2021/06/08 00:00 [revised]
PHST- 2021/06/15 00:00 [accepted]
PHST- 2021/06/24 06:00 [pubmed]
PHST- 2022/01/04 06:00 [medline]
PHST- 2021/06/23 07:25 [entrez]
AID - 6307734 [pii]
AID - 10.1093/biolre/ioab121 [doi]
PST - ppublish
SO  - Biol Reprod. 2021 Oct 11;105(4):846-858. doi: 10.1093/biolre/ioab121.