PMID- 34162950
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20240402
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jun 23
TI  - Fatty acid desaturase 2 (FADS 2) rs174575 (C/G) polymorphism, circulating lipid 
      levels and susceptibility to type-2 diabetes mellitus.
PG  - 13151
LID - 10.1038/s41598-021-92572-7 [doi]
LID - 13151
AB  - Several factors influence an individual's susceptibility in inter-individual 
      lipid changes and its role in the onset of type-2 diabetes mellitus (T2DM). 
      Considering the above fact, the present investigation focuses on determining the 
      association between fatty acid desaturase 2 (FADS2) rs174575 (C/G) polymorphism, 
      circulating lipid levels and susceptibility to type-2 diabetes mellitus. As per 
      the inclusion and exclusion criteria a total of 429 subjects (non-diabetic-216; 
      diabetic-213) were recruited for the study. Glycemic and lipid profile status 
      were assessed using commercially available kits. Based on the previous reports 
      SNP rs174575 of fatty acid desaturase gene (FADS2) was selected and identified 
      using the dbSNP database. The amplified products were sequenced by means of 
      Sanger sequencing method. Lipid profile status and apolipoprotein levels revealed 
      statistically significant difference between the groups. Three models were 
      assessed namely, recessive model (CC vs CG + GG), dominant model (CC + CG vs GG) 
      and additive model (CC vs CG vs GG). The recessive model, displayed a 
      statistically significant variations between the circulating lipid levels in 
      T2DM. The multivariate model with genotype (G allele carriers), triglyceride (TG) 
      and insulin served as a predictive model. The study results potentiate the 
      functional link between FADS2 gene polymorphism, lipid levels and type-2 diabetes 
      mellitus.
FAU - Shetty, Shilpa S
AU  - Shetty SS
AD  - Central Research Laboratory, K.S.Hegde Medical Academy, Nitte (Deemed To Be 
      University), Deralakatte, Mangalore, India.
FAU - Kumari, N Suchetha
AU  - Kumari NS
AD  - Department of Biochemistry, K.S.Hegde Medical Academy, Nitte (Deemed To Be 
      University), Deralakatte, Mangalore, India. kumarin@nitte.edu.in.
LA  - eng
PT  - Journal Article
DEP - 20210623
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Apolipoproteins)
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (Lipids)
RN  - 0 (Triglycerides)
RN  - EC 1.14.19.- (Fatty Acid Desaturases)
RN  - EC 1.14.19.3 (FADS2 protein, human)
SB  - IM
MH  - Adult
MH  - Apolipoproteins/blood
MH  - Blood Glucose/analysis
MH  - Diabetes Mellitus, Type 2/blood/enzymology/*genetics
MH  - Dyslipidemias/blood/enzymology/*genetics
MH  - Fatty Acid Desaturases/*genetics
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Insulin/blood
MH  - Lipids/*blood
MH  - Male
MH  - Middle Aged
MH  - Models, Genetic
MH  - *Polymorphism, Single Nucleotide
MH  - Triglycerides/blood
PMC - PMC8222307
COIS- The authors declare no competing interests.
EDAT- 2021/06/25 06:00
MHDA- 2021/11/03 06:00
PMCR- 2021/06/23
CRDT- 2021/06/24 06:12
PHST- 2021/02/23 00:00 [received]
PHST- 2021/06/02 00:00 [accepted]
PHST- 2021/06/24 06:12 [entrez]
PHST- 2021/06/25 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/06/23 00:00 [pmc-release]
AID - 10.1038/s41598-021-92572-7 [pii]
AID - 92572 [pii]
AID - 10.1038/s41598-021-92572-7 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jun 23;11(1):13151. doi: 10.1038/s41598-021-92572-7.