PMID- 34163351
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210625
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 12
DP  - 2021
TI  - Dietary Inorganic Nitrate Protects Hepatic Ischemia-Reperfusion Injury Through 
      NRF2-Mediated Antioxidative Stress.
PG  - 634115
LID - 10.3389/fphar.2021.634115 [doi]
LID - 634115
AB  - Objectives: Hepatic ischemia-reperfusion injury (HIRI) is of common occurrence 
      during liver surgery and liver transplantation and may cause hepatic impairment, 
      resulting in acute liver dysfunction. Nitrate plays an important physiological 
      regulatory role in the human body. Whether dietary nitrate could prevent HIRI is, 
      however, unknown. Methods: A HIRI mouse model was established in that the blood 
      supply to the median lobe and left lateral lobe was blocked for 60 min through 
      the portal vein and related structures using an atraumatic clip. Sodium nitrate 
      (4 mM) was administrated in advance through drinking water to compare the 
      influence of sodium nitrate and normal water on HIRI. Results: Liver necrosis and 
      injury aggravated after HIRI. The group treated with sodium nitrate showed the 
      lowest activities of plasma aminotransferase and lactate dehydrogenase and 
      improved outcomes in histological investigation and TUNEL assay. Mechanistically, 
      sodium nitrate intake increased plasma and liver nitric oxide levels, upregulated 
      nuclear factor erythroid 2-related factor 2 (NRF2)-related molecules to reduce 
      malondialdehyde level, and increased the activities of antioxidant enzymes to 
      modulate hepatic oxidative stress. Conclusions: Dietary inorganic nitrate could 
      prevent HIRI, possibly by activating the NRF2 pathway and modulating oxidative 
      stress. Our study provides a novel therapeutic compound that could potentially 
      prevent HIRI during liver transplantation or hepatic surgery.
CI  - Copyright (c) 2021 Li, Jin, Sun, Zhang, Wang, Xu, Zhang and Wang.
FAU - Li, Shaorong
AU  - Li S
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Beijing Laboratory of Oral Health, Capital Medical 
      University School of Stomatology, Beijing, China.
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
FAU - Jin, Hua
AU  - Jin H
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
AD  - Beijing Key Laboratory of Tolerance Induction and Organ Protection in 
      Transplantation, Beijing, China.
FAU - Sun, Guangyong
AU  - Sun G
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
AD  - Beijing Key Laboratory of Tolerance Induction and Organ Protection in 
      Transplantation, Beijing, China.
FAU - Zhang, Chunmei
AU  - Zhang C
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Beijing Laboratory of Oral Health, Capital Medical 
      University School of Stomatology, Beijing, China.
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
FAU - Wang, Jinsong
AU  - Wang J
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Beijing Laboratory of Oral Health, Capital Medical 
      University School of Stomatology, Beijing, China.
AD  - Department of Biochemistry and Molecular Biology, Capital Medical University 
      School of Basic Medicine, Beijing, China.
FAU - Xu, Hufeng
AU  - Xu H
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
AD  - Beijing Key Laboratory of Tolerance Induction and Organ Protection in 
      Transplantation, Beijing, China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
AD  - Beijing Key Laboratory of Tolerance Induction and Organ Protection in 
      Transplantation, Beijing, China.
FAU - Wang, Songlin
AU  - Wang S
AD  - Salivary Gland Disease Center and Beijing Key Laboratory of Tooth Regeneration 
      and Function Reconstruction, Beijing Laboratory of Oral Health, Capital Medical 
      University School of Stomatology, Beijing, China.
AD  - Immunology Research Center for Oral and Systemic Health, Beijing Friendship 
      Hospital, Capital Medical University, Beijing, China.
AD  - Department of Biochemistry and Molecular Biology, Capital Medical University 
      School of Basic Medicine, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20210607
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC8215696
OTO - NOTNLM
OT  - NRF2
OT  - hepatic ischemia reperfusion injury (HIRI)
OT  - nitrate
OT  - oral administration
OT  - oxidative stress
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/25 06:00
MHDA- 2021/06/25 06:01
PMCR- 2021/06/07
CRDT- 2021/06/24 06:33
PHST- 2020/11/27 00:00 [received]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/06/24 06:33 [entrez]
PHST- 2021/06/25 06:00 [pubmed]
PHST- 2021/06/25 06:01 [medline]
PHST- 2021/06/07 00:00 [pmc-release]
AID - 634115 [pii]
AID - 10.3389/fphar.2021.634115 [doi]
PST - epublish
SO  - Front Pharmacol. 2021 Jun 7;12:634115. doi: 10.3389/fphar.2021.634115. 
      eCollection 2021.