PMID- 34163516
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210625
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 12
DP  - 2021
TI  - Development and Molecular Cytogenetic Identification of a New 
      Wheat-Psathyrostachys huashanica Keng Translocation Line Resistant to Powdery 
      Mildew.
PG  - 689502
LID - 10.3389/fpls.2021.689502 [doi]
LID - 689502
AB  - Psathyrostachys huashanica Keng, a wild relative of common wheat with many 
      desirable traits, is an invaluable source of genetic material for wheat 
      improvement. Few wheat-P. huashanica translocation lines resistant to powdery 
      mildew have been reported. In this study, a wheat-P. huashanica line, 
      E24-3-1-6-2-1, was generated via distant hybridization, ethyl methanesulfonate 
      (EMS) mutagenesis, and backcross breeding. A chromosome karyotype of 2n = 44 was 
      observed at the mitotic stage in E24-3-1-6-2-1. Genomic in situ hybridization 
      (GISH) analysis revealed four translocated chromosomes in E24-3-1-6-2-1, and P. 
      huashanica chromosome-specific marker analysis showed that the alien chromosome 
      fragment was from the P. huashanica 4Ns chromosome. Moreover, fluorescence in 
      situ hybridization (FISH) analysis demonstrated that reciprocal translocation had 
      occurred between the P. huashanica 4Ns chromosome and the wheat 3D chromosome; 
      thus, E24-3-1-6-2-1 carried two translocations: T3DS.3DL-4NsL and T3DL-4NsS. 
      Translocation also occurred between wheat chromosomes 2A and 4A. At the adult 
      stage, E24-3-1-6-2-1 was highly resistant to powdery mildew, caused by prevalent 
      pathotypes in China. Further, the spike length, numbers of fertile spikelets, 
      kernels per spike, thousand-kernel weight, and grain yield of E24-3-1-6-2-1 were 
      significantly higher than those of its wheat parent 7182 and addition line 
      24-6-3-1. Thus, this translocation line that is highly resistant to powdery 
      mildew and has excellent agronomic traits can be used as a novel promising 
      germplasm for breeding resistant and high-yielding cultivars.
CI  - Copyright (c) 2021 Liu, Huang, Han, Hou, Zheng, Zhang and Wu.
FAU - Liu, Yuxiu
AU  - Liu Y
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
FAU - Huang, Shuhua
AU  - Huang S
AD  - College of Horticulture, Northwest A&F University, Yangling, China.
FAU - Han, Jing
AU  - Han J
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
FAU - Hou, Chenchen
AU  - Hou C
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
FAU - Zheng, Dasheng
AU  - Zheng D
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
FAU - Zhang, Zhengmao
AU  - Zhang Z
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
FAU - Wu, Jun
AU  - Wu J
AD  - College of Agronomy, Northwest A&F University, Yangling, China.
LA  - eng
PT  - Journal Article
DEP - 20210607
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC8215663
OTO - NOTNLM
OT  - P. huashanica
OT  - agronomic performance
OT  - translocation line
OT  - wheat
OT  - wheat powdery mildew
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/06/25 06:00
MHDA- 2021/06/25 06:01
PMCR- 2021/01/01
CRDT- 2021/06/24 06:34
PHST- 2021/04/01 00:00 [received]
PHST- 2021/05/18 00:00 [accepted]
PHST- 2021/06/24 06:34 [entrez]
PHST- 2021/06/25 06:00 [pubmed]
PHST- 2021/06/25 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2021.689502 [doi]
PST - epublish
SO  - Front Plant Sci. 2021 Jun 7;12:689502. doi: 10.3389/fpls.2021.689502. eCollection 
      2021.