PMID- 34166824
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 1878-8769 (Electronic)
IS  - 1878-8750 (Linking)
VI  - 153
DP  - 2021 Sep
TI  - Long Noncoding RNA Expression Profiles of Rat Extrasynaptic and Synaptic Neurons 
      Expressing the N-methyl-D-Aspartate Receptor Revealed by Microarray Analysis.
PG  - e168-e178
LID - S1878-8750(21)00901-3 [pii]
LID - 10.1016/j.wneu.2021.06.070 [doi]
AB  - PURPOSE: To study the 24-hour expression of long noncoding RNAs (lncRNAs) in 
      synaptic and extrasynaptic neurons expressing N-methyl-D-aspartate receptor 
      (NMDAR), and normal neuronal cultures, via microarray analysis. MATERIALS AND 
      METHODS: Cortical neurons from embryonic (day E18) Sprague-Dawley rats were used 
      for primary neuronal culture. NMDAR activation was blocked and the cells were 
      then incubated for 6 hours. Total RNA was extracted, quantified, and analyzed for 
      purity and integrity. Double-stranded cDNA was synthesized, followed by quantile 
      normalization, quantitative polymerase chain reaction validation, and data 
      analysis. The interactions between transcription factors and lncRNAs were 
      analyzed by Pearson correlation. RESULTS: The lncRNA profiles were obtained after 
      synaptic and extrasynaptic NMDAR activation of rat cortical neuron cultures for 
      24 hours. In total, 251 lncRNAs were consistently upregulated, and 335 were 
      downregulated, after extrasynaptic NMDAR activation compared with normal neurons. 
      After synaptic NMDAR activation, only 9 lncRNAs were upregulated and 2 were 
      downregulated. CONCLUSIONS: Differential expression of lncRNAs after synaptic and 
      extrasynaptic NMDAR activation suggests that lncRNAs may be responsible for 
      extrasynaptic NMDAR-induced neurodegeneration.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Wang, Huan
AU  - Wang H
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Hu, Shunze
AU  - Hu S
AD  - Department of Pathology, Maternal and Children's Hospital of Hubei Province, 
      Wuhan, China.
FAU - Gao, Shutao
AU  - Gao S
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Chen, Kun
AU  - Chen K
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Sun, Xuying
AU  - Sun X
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Fang, Huang
AU  - Fang H
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China. Electronic address: 
      fanghuangtjh@hust.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210622
PL  - United States
TA  - World Neurosurg
JT  - World neurosurgery
JID - 101528275
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (GABA-A Receptor Antagonists)
RN  - 0 (Glycine Agents)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6384-92-5 (N-Methylaspartate)
RN  - BH3B64OKL9 (4-Aminopyridine)
RN  - TE7660XO1C (Glycine)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 4-Aminopyridine/pharmacology
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - Cerebral Cortex/cytology
MH  - Down-Regulation
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - GABA-A Receptor Antagonists/pharmacology
MH  - Glycine/pharmacology
MH  - Glycine Agents/pharmacology
MH  - Microarray Analysis
MH  - N-Methylaspartate/pharmacology
MH  - Neurons/*metabolism
MH  - Polymerase Chain Reaction
MH  - Potassium Channel Blockers/pharmacology
MH  - Primary Cell Culture
MH  - RNA, Long Noncoding/*metabolism
MH  - Rats
MH  - Receptors, N-Methyl-D-Aspartate/drug effects/*metabolism
MH  - Synapses/drug effects/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Long noncoding RNAs
OT  - Microarray analysis
OT  - NMDA receptor
OT  - Synapse
EDAT- 2021/06/25 06:00
MHDA- 2021/11/09 06:00
CRDT- 2021/06/24 20:13
PHST- 2021/04/16 00:00 [received]
PHST- 2021/06/14 00:00 [revised]
PHST- 2021/06/14 00:00 [accepted]
PHST- 2021/06/25 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/06/24 20:13 [entrez]
AID - S1878-8750(21)00901-3 [pii]
AID - 10.1016/j.wneu.2021.06.070 [doi]
PST - ppublish
SO  - World Neurosurg. 2021 Sep;153:e168-e178. doi: 10.1016/j.wneu.2021.06.070. Epub 
      2021 Jun 22.