PMID- 3416813
OWN - NLM
STAT- MEDLINE
DCOM- 19881027
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 123
IP  - 4
DP  - 1988 Oct
TI  - Regional sex differences in cell nuclear estrogen-binding capacity in the rat 
      hypothalamus and preoptic area.
PG  - 1761-70
AB  - Estrogen binding was compared in cell nuclear KCl extracts from microdissected 
      brain regions of gonadectomized-adrenalectomized male and female rats treated 
      with a near-saturating dose of 17 beta-estradiol. Injection of 3.6 or 36.0 
      micrograms 17 beta-estradiol/kg BW, iv, 1 h before death resulted in a higher 
      level of estrogen binding in the periventricular preoptic area (PVPOA), medial 
      preoptic area, and ventromedial nucleus of the hypothalamus (VMN) of the female 
      than in comparable tissue samples from the male. No significant sex differences 
      in nuclear estrogen binding were observed in the arcuate-median eminence region, 
      bed nucleus of the stria terminalis, or corticomedial amygdala. Scatchard 
      analysis of saturation binding data revealed that the sex differences in cell 
      nuclear estrogen binding in the PVPOA, medial preoptic area, and VMN reflect a 
      difference in binding capacity rather than binding affinity. These in vitro 
      biochemical findings were confirmed by autoradiographic studies. 
      Gonadectomized-adrenalectomized animals were injected with 125I-labeled 11 
      beta-methoxy-16 alpha-iodoestradiol (2.0 micrograms/kg BW). Thin frozen sections 
      (10 microns) through the preoptic area and hypothalamus were thaw-mounted onto 
      microscope slides, then exposed against LKB Ultrofilm for 21 days. The 
      autoradiographic images exhibited similar silver distributions and densities in 
      males and females in the arcuate-median eminence region bed nucleus of the stria 
      terminalis, and amygdala. However, 11 beta-[125I]methoxy-16 alpha-iodoestradiol 
      uptake was lower in males than in females in the PVPOA and VMN. These results 
      suggest that sex differences in responsiveness to estrogen stimulation in the rat 
      may be due in part to sex differences in estrogen-binding capacity in specific 
      regions of the hypothalamus that play important roles in the control of pituitary 
      function and reproductive behaviors.
FAU - Brown, T J
AU  - Brown TJ
AD  - Department of Obstetrics and Gynecology, Yale University School of Medicine, New 
      Haven, Connecticut 06510.
FAU - Hochberg, R B
AU  - Hochberg RB
FAU - Zielinski, J E
AU  - Zielinski JE
FAU - MacLusky, N J
AU  - MacLusky NJ
LA  - eng
GR  - CA-37799/CA/NCI NIH HHS/United States
GR  - HD-13587/HD/NICHD NIH HHS/United States
GR  - NS-07807/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Receptors, Estrogen)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Adrenalectomy
MH  - Animals
MH  - Cell Nucleus/*metabolism
MH  - Estradiol/metabolism/pharmacology
MH  - Female
MH  - Hypothalamus/*metabolism
MH  - Male
MH  - Orchiectomy
MH  - Organ Specificity
MH  - Ovariectomy
MH  - Preoptic Area/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Estrogen/drug effects/*metabolism
MH  - Reference Values
MH  - Sex Factors
EDAT- 1988/10/01 00:00
MHDA- 1988/10/01 00:01
CRDT- 1988/10/01 00:00
PHST- 1988/10/01 00:00 [pubmed]
PHST- 1988/10/01 00:01 [medline]
PHST- 1988/10/01 00:00 [entrez]
AID - 10.1210/endo-123-4-1761 [doi]
PST - ppublish
SO  - Endocrinology. 1988 Oct;123(4):1761-70. doi: 10.1210/endo-123-4-1761.