PMID- 34173250
OWN - NLM
STAT- MEDLINE
DCOM- 20210914
LR  - 20210914
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 73
IP  - 3
DP  - 2021 Sep
TI  - Cell-free culture supernatant of Lactobacillus curvatus Wikim38 inhibits 
      RANKL-induced osteoclast differentiation and ameliorates bone loss in 
      ovariectomized mice.
PG  - 383-391
LID - 10.1111/lam.13525 [doi]
AB  - This study was conducted to investigate the inhibitory effects of the cell-free 
      culture supernatant of Lactobacillus curvatus Wikim 38 (LC38-CS) on RANKL-induced 
      osteoclast differentiation and bone loss in a mice model of ovariectomy-induced 
      post-menopausal osteoporosis. LC38-CS inhibited the RANKL-induced differentiation 
      of bone marrow-derived macrophages (BMDMs) into osteoclasts in a dose-dependent 
      manner. F-actin ring formation and bone resorption were also reduced by LC38-CS 
      treatment of RANKL-treated BMDMs. In addition, LC38-CS decreased the 
      RANKL-induced activation of the TRAF6/NF-kappaB/MAPKs axis at the early stage and the 
      expression of osteoclastogenesis-related genes in BMDMs treated with RANKL. PRMT1 
      and ADMA levels, new biomarkers for osteoclastogenesis, were decreased by LC38-CS 
      treatment. The administration of LC38-CS increased bone volume and bone mineral 
      density in ovariectomized mice in mu-CT analysis. These findings suggest that 
      LC38-CS inhibited RANKL-induced osteoclast differentiation by the downregulation 
      of molecular mechanisms and exerted anti-osteoporotic effects.
CI  - (c) 2021 The Society for Applied Microbiology.
FAU - Jang, A-R
AU  - Jang AR
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
FAU - Park, J-S
AU  - Park JS
AD  - Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, 
      Republic of Korea.
FAU - Kim, D-K
AU  - Kim DK
AD  - Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, 
      Republic of Korea.
FAU - Park, J-Y
AU  - Park JY
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
FAU - Ahn, J-H
AU  - Ahn JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
FAU - Kim, D-Y
AU  - Kim DY
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
FAU - Lee, T-S
AU  - Lee TS
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
FAU - Chang, J-Y
AU  - Chang JY
AD  - Microbiology and Functionality Research Group, World Institute of Kimchi, 
      Gwangju, Republic of Korea.
FAU - Choi, J-H
AU  - Choi JH
AD  - Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, 
      Republic of Korea.
FAU - Park, J-H
AU  - Park JH
AUID- ORCID: 0000-0003-4664-5640
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Republic of Korea.
LA  - eng
GR  - P0008590/Korea Institute for Advancement of Technology/
PT  - Journal Article
DEP - 20210707
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (NF-kappa B)
RN  - Lactobacillus curvatus
SB  - IM
MH  - Animals
MH  - *Bone Resorption
MH  - Cell Differentiation
MH  - Female
MH  - Lactobacillus
MH  - Mice
MH  - NF-kappa B
MH  - *Osteoclasts
OTO - NOTNLM
OT  - Lactobacillus curvatus
OT  - RANKL-induced osteoclast
OT  - osteoporosis
OT  - ovariectomized mice
OT  - postbiotics supplementation
EDAT- 2021/06/27 06:00
MHDA- 2021/09/15 06:00
CRDT- 2021/06/26 06:23
PHST- 2021/06/22 00:00 [revised]
PHST- 2021/03/29 00:00 [received]
PHST- 2021/06/22 00:00 [accepted]
PHST- 2021/06/27 06:00 [pubmed]
PHST- 2021/09/15 06:00 [medline]
PHST- 2021/06/26 06:23 [entrez]
AID - 10.1111/lam.13525 [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2021 Sep;73(3):383-391. doi: 10.1111/lam.13525. Epub 2021 
      Jul 7.