PMID- 34174607
OWN - NLM
STAT- MEDLINE
DCOM- 20210906
LR  - 20210906
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 793
DP  - 2021 Nov 1
TI  - Deep-sea plastisphere: Long-term colonization by plastic-associated bacterial and 
      archaeal communities in the Southwest Atlantic Ocean.
PG  - 148335
LID - S0048-9697(21)03406-9 [pii]
LID - 10.1016/j.scitotenv.2021.148335 [doi]
AB  - Marine plastic pollution is a global concern because of continuous release into 
      the oceans over the last several decades. Although recent studies have made 
      efforts to characterize the so-called plastisphere, or microbial community 
      inhabiting plastic substrates, it is not clear whether the plastisphere is 
      defined as a core community or as a random attachment of microbial cells. 
      Likewise, little is known about the influence of the deep-sea environment on the 
      plastisphere. In our experimental study, we evaluated the microbial colonization 
      on polypropylene pellets and two types of plastic bags: regular high density 
      polyethylene (HDPE) and HDPE with the oxo-biodegradable additive BDA. Gravel was 
      used as control. Samples were deployed at three sites at 3300 m depth in the 
      Southwest Atlantic Ocean and left for microbial colonization for 719 days. For 
      microbial communities analysis, DNA was extracted from the biofilm on plastic and 
      gravel substrates, and then the 16S rRNA was sequenced through the Illumina Miseq 
      platform. Cultivation was performed to isolate strains from the plastic and 
      gravel substrates. Substrate type strongly influenced the microbial composition 
      and structure, while no difference between sites was detected. Although several 
      taxa were shared among plastics, we observed some groups specific for each 
      plastic substrate. These communities comprised taxa previously reported from both 
      epipelagic zones and deep-sea benthic ecosystems. The core microbiome (microbial 
      taxa shared by all plastic substrates) was exclusively composed by low abundance 
      taxa, with some members well-described in the plastisphere and with known 
      plastic-degradation capabilities. Additionally, we obtained bacterial strains 
      that have been previously reported inhabiting plastic substrates and/or degrading 
      hydrocarbon compounds, which corroborates our metabarcoding data and suggests the 
      presence of microbial members potentially active and involved with degradation of 
      these plastics in the deep sea.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Agostini, Luana
AU  - Agostini L
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Moreira, Julio Cezar Fornazier
AU  - Moreira JCF
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil. Electronic address: jfornazier@usp.br.
FAU - Bendia, Amanda Goncalves
AU  - Bendia AG
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Kmit, Maria Carolina Pezzo
AU  - Kmit MCP
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Waters, Linda Gwen
AU  - Waters LG
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Santana, Marina Ferreira Mourao
AU  - Santana MFM
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Sumida, Paulo Yukio Gomes
AU  - Sumida PYG
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Turra, Alexander
AU  - Turra A
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
FAU - Pellizari, Vivian Helena
AU  - Pellizari VH
AD  - Instituto Oceanografico, Universidade de Sao Paulo, Praca do Oceanografico, 191, 
      Sao Paulo CEP: 05508-120, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210608
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Plastics)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Archaea/genetics
MH  - Atlantic Ocean
MH  - *Microbiota
MH  - *Plastics
MH  - RNA, Ribosomal, 16S
OTO - NOTNLM
OT  - 16S rRNA
OT  - Core microbiome
OT  - Microbial colonization
OT  - Plastics
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/06/27 06:00
MHDA- 2021/09/07 06:00
CRDT- 2021/06/26 20:20
PHST- 2021/01/14 00:00 [received]
PHST- 2021/05/20 00:00 [revised]
PHST- 2021/06/04 00:00 [accepted]
PHST- 2021/06/27 06:00 [pubmed]
PHST- 2021/09/07 06:00 [medline]
PHST- 2021/06/26 20:20 [entrez]
AID - S0048-9697(21)03406-9 [pii]
AID - 10.1016/j.scitotenv.2021.148335 [doi]
PST - ppublish
SO  - Sci Total Environ. 2021 Nov 1;793:148335. doi: 10.1016/j.scitotenv.2021.148335. 
      Epub 2021 Jun 8.