PMID- 34175439
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20210826
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 172
DP  - 2021 Aug 20
TI  - Temporal characterization of mitochondrial impairment in the unilateral ureteral 
      obstruction model in rats.
PG  - 358-371
LID - S0891-5849(21)00384-1 [pii]
LID - 10.1016/j.freeradbiomed.2021.06.019 [doi]
AB  - Renal fibrosis is a well-known mechanism that favors chronic kidney disease (CKD) 
      development in obstructive nephropathy, a significant pathology worldwide. 
      Fibrosis induction involves several pathways, and although mitochondrial 
      alterations have recently emerged as a critical factor that triggers renal damage 
      in the obstructed kidney, the temporal mitochondrial alterations during the 
      fibrotic induction remain unexplored. Therefore, in this work, we evaluated the 
      time course of mitochondrial mass and bioenergetics alterations induced by a 
      unilateral ureteral obstruction (UUO), a widely used model to study the mechanism 
      involved in kidney fibrosis induction and progression. Our results show a marked 
      reduction in mitochondrial oxidative phosphorylation (OXPHOS) in the obstructed 
      kidney on days 7 to 28 of obstruction without significant mitochondrial coupling 
      changes. Besides, we observed that mitochondrial mass was reduced, probably due 
      to decreased biogenesis and mitophagy induction. OXPHOS impairment was associated 
      with decreased mitochondrial biogenesis markers, the peroxisome 
      proliferator-activated receptor gamma co-activator-1alpha (PGC-1alpha), and nuclear 
      respiratory factor 1 (NRF1); and also, with the induction of mitophagy in a 
      PTEN-induced kinase 1 (PINK1) and Parkin independent way. It is concluded that 
      the impairment of OXPHOS capacity may be explained by the reduction in 
      mitochondrial biogenesis and the induction of mitophagy during fibrotic 
      progression.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Jimenez-Uribe, Alexis Paulina
AU  - Jimenez-Uribe AP
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico, CDMX 04510, Mexico.
FAU - Bellido, Belen
AU  - Bellido B
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico, CDMX 04510, Mexico.
FAU - Aparicio-Trejo, Omar Emiliano
AU  - Aparicio-Trejo OE
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico, CDMX 04510, Mexico.
FAU - Tapia, Edilia
AU  - Tapia E
AD  - Departmento de Patofisiologia Cardio-renal, Instituto Nacional de Cardiologia 
      "Ignacio Chavez", Mexico City 14080, Mexico.
FAU - Sanchez-Lozada, Laura Gabriela
AU  - Sanchez-Lozada LG
AD  - Departmento de Patofisiologia Cardio-renal, Instituto Nacional de Cardiologia 
      "Ignacio Chavez", Mexico City 14080, Mexico.
FAU - Hernandez-Santos, Jose Antonio
AU  - Hernandez-Santos JA
AD  - Laboratorio de Neurobiologia Molecular y Celular, Instituto Nacional de 
      Neurologia y Neurocirugia, Manuel Velasco Suarez, Av. Insurgentes Sur # 3877, La 
      Fama, Alcaldia Tlalpan, CP 14269, Ciudad de Mexico, Mexico.
FAU - Fernandez-Valverde, Francisca
AU  - Fernandez-Valverde F
AD  - Laboratorio de Patologia Experimental, Instituto Nacional de Neurologia y 
      Neurocirugia, Manuel Velasco Suarez, Av. Insurgentes Sur # 3877, La Fama, 
      Alcaldia Tlalpan, CP 14269, Ciudad de Mexico, Mexico.
FAU - Hernandez-Cruz, Estefani Yaquelin
AU  - Hernandez-Cruz EY
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico, CDMX 04510, Mexico.
FAU - Orozco-Ibarra, Marisol
AU  - Orozco-Ibarra M
AD  - Laboratorio de Neurobiologia Molecular y Celular, Instituto Nacional de 
      Neurologia y Neurocirugia, Manuel Velasco Suarez, Av. Insurgentes Sur # 3877, La 
      Fama, Alcaldia Tlalpan, CP 14269, Ciudad de Mexico, Mexico.
FAU - Pedraza-Chaverri, Jose
AU  - Pedraza-Chaverri J
AD  - Facultad de Quimica, Departamento de Biologia, Universidad Nacional Autonoma de 
      Mexico, CDMX 04510, Mexico. Electronic address: pedraza@unam.mx.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210624
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
SB  - IM
MH  - Animals
MH  - Fibrosis
MH  - Mitochondria
MH  - Mitophagy
MH  - Organelle Biogenesis
MH  - Rats
MH  - *Ureteral Obstruction
OTO - NOTNLM
OT  - Mitochondrial bioenergetics alterations
OT  - Mitochondrial biogenesis
OT  - Mitochondrial dysfunction
OT  - Mitophagy
OT  - Renal fibrosis
OT  - Unilateral ureteral obstruction
EDAT- 2021/06/28 06:00
MHDA- 2021/08/27 06:00
CRDT- 2021/06/27 20:42
PHST- 2021/05/05 00:00 [received]
PHST- 2021/05/31 00:00 [revised]
PHST- 2021/06/21 00:00 [accepted]
PHST- 2021/06/28 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2021/06/27 20:42 [entrez]
AID - S0891-5849(21)00384-1 [pii]
AID - 10.1016/j.freeradbiomed.2021.06.019 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2021 Aug 20;172:358-371. doi: 
      10.1016/j.freeradbiomed.2021.06.019. Epub 2021 Jun 24.