PMID- 34175651
OWN - NLM
STAT- MEDLINE
DCOM- 20210819
LR  - 20210819
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 191
DP  - 2021 Nov 1
TI  - High selectivity detection of FMDV- SAT-2 using a newly-developed electrochemical 
      nanosensors.
PG  - 113435
LID - S0956-5663(21)00472-3 [pii]
LID - 10.1016/j.bios.2021.113435 [doi]
AB  - Foot-and-mouth disease virus serotype South-Africa territories-2 (FMDV-SAT-2) is 
      the most fastidious known type in Aphthovirus which is subsequently reflected in 
      the diagnosis regime. Rapid and early diagnostic actions are usually taken in 
      response to the FMDV outbreak to prevent the dramatic spread of the disease. 
      Virus imprinted sensor (VIP sensor) is gathering huge attention for the selective 
      detection of pathogens. Thus, the whole virus particles of SAT-2 together with an 
      electropolymerized film of poly(o-phenylenediamine) (PoPD) on gold-copper 
      modified screen-printed electrode were applied to fabricate SAT-2-virus imprinted 
      polymer (SAT-2-VIP). The SAT-2-VIPs were fully characterized using cyclic 
      voltammetry (CV), linear sweep voltammetry (LSV), Atomic force microscopy (AFM), 
      Scanning electron microscope (SEM), and Fourier transform Infra-Red (FTIR) 
      spectroscopy. Excellent selective binding affinity towards the targeted virus 
      particle was achieved with limits of detection and quantification of 0.1 ng/mL 
      and 0.4 ng/mL, respectively. In terms of viral interference, the sensor did not 
      show cross-reactivity towards other animal viruses including FMDV serotype A, O, 
      or even SAT-2 subtype Libya and the un-related virus Lumpy skin disease virus 
      (LSDV). This high selectivity provides a sensible platform with 70 folds more 
      sensitivity than the reference RT-PCR as revealed from the application of 
      SAT-2-VIP sensor for rapid analysis of clinical samples with no need for 
      treatment or equipped labs. Thus, as diagnostic and surveillance technologies, 
      on-site point of care diagnostics for SAT-2 virus are supported.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Hussein, Heba A
AU  - Hussein HA
AD  - Center for Materials Science, Zewail City of Science and Technology, 6th October 
      City, 12578, Giza, Egypt; Virology Department, Animal Health Research Institute 
      (AHRI), Agricultural Research Center (ARC), Giza, 12619, Egypt.
FAU - El Nashar, Rasha Mohamed
AU  - El Nashar RM
AD  - Chemistry Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.
FAU - El-Sherbiny, Ibrahim M
AU  - El-Sherbiny IM
AD  - Center for Materials Science, Zewail City of Science and Technology, 6th October 
      City, 12578, Giza, Egypt. Electronic address: ielsherbiny@zewailcity.edu.eg.
FAU - Hassan, Rabeay Y A
AU  - Hassan RYA
AD  - Center for Materials Science, Zewail City of Science and Technology, 6th October 
      City, 12578, Giza, Egypt; Applied Organic Chemistry Department, National Research 
      Centre (NRC), Dokki, 12622, Giza, Egypt. Electronic address: 
      ryounes@zewailcity.edu.eg.
LA  - eng
PT  - Journal Article
DEP - 20210624
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Animals
MH  - *Biosensing Techniques
MH  - Cattle
MH  - Disease Outbreaks
MH  - *Foot-and-Mouth Disease
MH  - *Foot-and-Mouth Disease Virus
MH  - Serogroup
OTO - NOTNLM
OT  - Foot-and-mouth disease virus (FMDV)
OT  - Metal nanoparticles
OT  - On-site diagnosis
OT  - SAT-2 virus imprinting
OT  - Virus biosensors
EDAT- 2021/06/28 06:00
MHDA- 2021/08/20 06:00
CRDT- 2021/06/27 20:51
PHST- 2021/02/22 00:00 [received]
PHST- 2021/06/07 00:00 [revised]
PHST- 2021/06/10 00:00 [accepted]
PHST- 2021/06/28 06:00 [pubmed]
PHST- 2021/08/20 06:00 [medline]
PHST- 2021/06/27 20:51 [entrez]
AID - S0956-5663(21)00472-3 [pii]
AID - 10.1016/j.bios.2021.113435 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2021 Nov 1;191:113435. doi: 10.1016/j.bios.2021.113435. Epub 
      2021 Jun 24.