PMID- 34180734
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20220716
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 33
IP  - 5
DP  - 2021 Sep
TI  - PRRSV2 genetic diversity defined by RFLP patterns in the United States from 2007 
      to 2019.
PG  - 920-931
LID - 10.1177/10406387211027221 [doi]
AB  - The genetic diversity of porcine reproductive and respiratory syndrome virus 
      (PRRSV) increases over time. In 1998, restriction-fragment length polymorphism 
      (RFLP) pattern analysis was introduced to differentiate PRRSV wild-type strains 
      from VR2332, a reference strain from which a commercial vaccine (Ingelvac PRRS 
      MLV) was derived. We have characterized here the PRRSV genetic diversity within 
      selected RFLP families over time and U.S. geographic space, using available 
      ISU-VDL data from 2007 to 2019. The 40,454 ORF5 sequences recovered corresponded 
      to 228 distinct RFLPs. Four RFLPs [2-5-2 (21.2%), 1-7-4 (15.6%), 1-4-4 (11.8%), 
      and 1-8-4 (9.9%)] represented 58.5% of all ORF5 sequences and were used for 
      cluster analysis. Over time, there was increased detection of RFLPs 2-5-2, 1-7-4, 
      1-3-4, 1-3-2, and 1-12-4; decreased detection of 1-4-2, 1-18-4, 1-18-2, and 
      1-2-2; and different detection trends for 1-8-4, 1-4-4, 1-26-1, 1-22-2, and 
      1-2-4. An over-time cluster analysis revealed a single cluster for RFLP 2-5-2, 
      supporting that sequences within RFLP 2-5-2 are still relatively conserved. For 
      1-7-4, 1-4-4, and 1-8-4, there were multiple clusters. State-wise cluster 
      analysis demonstrated 4 main clusters for RFLP 1-7-4 and 1-8-4, and 6 for RFLP 
      1-4-4. For the other RFLPs, there was a significant genetic difference within 
      them, particularly between states. RFLP typing is limited in its ability to 
      discriminate among different strains of PRRSV. Understanding the magnitude of 
      genetic divergence within RFLPs helps develop PRRSV regional control programs, 
      placement, herd immunization strategies, and design of appropriate animal 
      movements across borders to minimize the risk of PRRSV transmission.
FAU - Trevisan, Giovani
AU  - Trevisan G
AUID- ORCID: 0000-0002-4980-526X
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Sharma, Aditi
AU  - Sharma A
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Gauger, Phillip
AU  - Gauger P
AUID- ORCID: 0000-0003-2540-8769
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Harmon, Karen M
AU  - Harmon KM
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Zhang, Jianqiang
AU  - Zhang J
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Main, Rodger
AU  - Main R
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Zeller, Michael
AU  - Zeller M
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Linhares, Leticia C M
AU  - Linhares LCM
AUID- ORCID: 0000-0001-5305-7981
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
FAU - Linhares, Daniel C L
AU  - Linhares DCL
AD  - Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 
      Medicine, Iowa State University, Ames, IA, USA.
LA  - eng
PT  - Journal Article
DEP - 20210628
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
SB  - IM
MH  - Animals
MH  - Genetic Variation
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - *Porcine Reproductive and Respiratory Syndrome/epidemiology
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - *Swine Diseases
MH  - United States/epidemiology
PMC - PMC8366264
OTO - NOTNLM
OT  - ORF5
OT  - PRRSV
OT  - RFLP
OT  - pairwise distance
OT  - phylogenetic
OT  - swine
COIS- Declaration of conflicting interests: The authors declared no potential conflicts 
      of interest with respect to the research, authorship, and/or publication of this 
      article.
EDAT- 2021/06/29 06:00
MHDA- 2021/10/16 06:00
PMCR- 2022/06/28
CRDT- 2021/06/28 12:14
PHST- 2021/06/29 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2021/06/28 12:14 [entrez]
PHST- 2022/06/28 00:00 [pmc-release]
AID - 10.1177_10406387211027221 [pii]
AID - 10.1177/10406387211027221 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2021 Sep;33(5):920-931. doi: 10.1177/10406387211027221. Epub 
      2021 Jun 28.