PMID- 34185082
OWN - NLM
STAT- MEDLINE
DCOM- 20211124
LR  - 20211124
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Linking)
VI  - 72
IP  - 20
DP  - 2021 Oct 26
TI  - Two citrus KNAT-like genes, CsKN1 and CsKN2, are involved in the regulation of 
      spring shoot development in sweet orange.
PG  - 7002-7019
LID - 10.1093/jxb/erab311 [doi]
AB  - Shoot-tip abortion is a very common phenomenon in some perennial woody plants and 
      it affects the height, architecture, and branch orientation of trees; however, 
      little is currently known about the underlying mechanisms. In this study, we 
      identified a gene in sweet orange (Citrus sinensis) encoding a KNAT-like protein 
      (CsKN1) and found high expression in the shoot apical meristem (SAM). 
      Overexpression of CsKN1 in transgenic plants prolonged the vegetative growth of 
      SAMs, whilst silencing resulted in either the loss or inhibition of SAMs. Yeast 
      two-hybrid analysis revealed that CsKN1 interacted with another citrus KNAT-like 
      protein (CsKN2), and overexpression of CsKN2 in lemon and tobacco caused an 
      extreme multiple-meristem phenotype. Overexpression of CsKN1 and CsKN2 in 
      transgenic plants resulted in the differential expression of numerous genes 
      related to hormone biosynthesis and signaling. Yeast one-hybrid analysis revealed 
      that the CsKN1-CsKN2 complex can bind to the promoter of citrus floral meristem 
      gene LEAFY (CsLFY) and inhibit its expression. These results indicated that CsKN1 
      might prolong the vegetative growth period of SAMs by delaying flowering. In 
      addition, an ethylene-responsive factor (CsERF) was found to bind to the CsKN1 
      promoter and suppresses its transcription. Overexpression of CsERF in Arabidopsis 
      increased the contents of ethylene and reactive oxygen species, which might 
      induce the occurrence of shoot-tip abscission. On the basis of our results, we 
      conclude that CsKN1 and CsKN2 might work cooperatively to regulate the shoot-tip 
      abscission process in spring shoots of sweet orange.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of the 
      Society for Experimental Biology. All rights reserved. For permissions, please 
      email: journals.permissions@oup.com.
FAU - Zeng, Ren-Fang
AU  - Zeng RF
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Zhou, Huan
AU  - Zhou H
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Fu, Li-Ming
AU  - Fu LM
AD  - College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Yan, Zhen
AU  - Yan Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Ye, Li-Xia
AU  - Ye LX
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Hu, Si-Fan
AU  - Hu SF
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Gan, Zhi-Meng
AU  - Gan ZM
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Ai, Xiao-Yan
AU  - Ai XY
AD  - Institute of Pomology and Tea, Hubei Academy of Agricultural Sciences, Wuhan, 
      China.
FAU - Hu, Chun-Gen
AU  - Hu CG
AUID- ORCID: 0000-0003-0178-468X
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
FAU - Zhang, Jin-Zhi
AU  - Zhang JZ
AUID- ORCID: 0000-0002-3820-889X
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Plant Proteins)
SB  - IM
MH  - *Citrus/genetics/metabolism
MH  - *Citrus sinensis/genetics/metabolism
MH  - Gene Expression Regulation, Plant
MH  - Meristem/genetics/metabolism
MH  - Plant Proteins/genetics/metabolism
OTO - NOTNLM
OT  - BREVIPEDICELLUS/KNAT1
OT  - Citrus
OT  - SHOOT MERISTEMLESS
OT  - sinensis
OT  - Auxin
OT  - ethylene
OT  - shoot apical meristem
OT  - shoot tip abscission
OT  - sweet orange
EDAT- 2021/06/30 06:00
MHDA- 2021/11/25 06:00
CRDT- 2021/06/29 12:18
PHST- 2021/02/05 00:00 [received]
PHST- 2021/06/26 00:00 [accepted]
PHST- 2021/06/30 06:00 [pubmed]
PHST- 2021/11/25 06:00 [medline]
PHST- 2021/06/29 12:18 [entrez]
AID - 6311289 [pii]
AID - 10.1093/jxb/erab311 [doi]
PST - ppublish
SO  - J Exp Bot. 2021 Oct 26;72(20):7002-7019. doi: 10.1093/jxb/erab311.