PMID- 34187291
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20211214
IS  - 1369-1651 (Electronic)
IS  - 0899-0220 (Linking)
VI  - 38
IP  - 3
DP  - 2021 Sep
TI  - Intracellular potassium depletion enhances apoptosis induced by staurosporine in 
      cultured trigeminal satellite glial cells.
PG  - 194-201
LID - 10.1080/08990220.2021.1941843 [doi]
AB  - PURPOSE: Satellite glial cells (SGC) surrounding neurons in sensory ganglia can 
      buffer extracellular potassium, regulating the excitability of injured neurons 
      and possibly influencing a shift from acute to neuropathic pain. SGC apoptosis 
      may be a key component in this process. This work evaluated induction or 
      enhancement of apoptosis in cultured trigeminal SGC following changes in 
      intracellular potassium [K]ic. MATERIALS AND METHODS: We developed SGC primary 
      cultures from rat trigeminal ganglia (TG). Purity of our cultures was confirmed 
      using immunofluorescence and western blot analysis for the presence of the 
      specific marker of SGC, glutamine synthetase (GS). SGC [K]ic was depleted using 
      hypo-osmotic shock and 4 mM bumetanide plus 10 mM ouabain. [K]ic was measured 
      using the K(+) fluorescent indicator potassium benzofuran isophthalate (PBFI-AM). 
      RESULTS: SGC tested positive for GS and hypo-osmotic shock induced a significant 
      decrease in [K]ic at every evaluated time. Cells were then incubated for 5 h with 
      either 2 mM staurosporine (STS) or 20 ng/ml of TNF-alpha and evaluated for early 
      apoptosis and late apoptosis/necrosis by flow cytometry using annexin V and 
      propidium iodide. A significant increase in early apoptosis, from 16 to 38%, was 
      detected in SGC with depleted [K]ic after incubation with STS. In contrast, TNF-alpha 
      did not increase early apoptosis in normal or [K]ic depleted SGC. CONCLUSION: 
      Hypo-osmotic shock induced a decrease in intracellular potassium in cultured 
      trigeminal SGC and this enhanced apoptosis induced by STS that is associated with 
      the mitochondrial pathway. These results suggest that K(+) dysregulation may 
      underlie apoptosis in trigeminal SGC.
FAU - Bustamante, Hedie A
AU  - Bustamante HA
AD  - Faculty of Veterinary Sciences, Veterinary Clinical Sciences Institute, 
      Universidad Austral de Chile, Valdivia, Chile.
FAU - Ehrich, Marion F
AU  - Ehrich MF
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
FAU - Klein, Bradley G
AU  - Klein BG
AD  - Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of 
      Veterinary Medicine, Virginia Polytechnic Institute and State University, 
      Blacksburg, VA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210630
PL  - England
TA  - Somatosens Mot Res
JT  - Somatosensory & motor research
JID - 8904127
RN  - H88EPA0A3N (Staurosporine)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - *Neuroglia
MH  - Potassium
MH  - Rats
MH  - Staurosporine/pharmacology
MH  - *Trigeminal Ganglion
OTO - NOTNLM
OT  - Satellite glial cells
OT  - apoptosis
OT  - neuropathic pain
OT  - staurosporine
EDAT- 2021/07/01 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/06/30 05:30
PHST- 2021/07/01 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/06/30 05:30 [entrez]
AID - 10.1080/08990220.2021.1941843 [doi]
PST - ppublish
SO  - Somatosens Mot Res. 2021 Sep;38(3):194-201. doi: 10.1080/08990220.2021.1941843. 
      Epub 2021 Jun 30.