PMID- 34187411
OWN - NLM
STAT- MEDLINE
DCOM- 20211019
LR  - 20211019
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jun 29
TI  - TINCR inhibits the proliferation and invasion of laryngeal squamous cell 
      carcinoma by regulating miR-210/BTG2.
PG  - 753
LID - 10.1186/s12885-021-08513-0 [doi]
LID - 753
AB  - BACKGROUND: Terminal differentiation-induced ncRNA (TINCR) plays an essential 
      role in epidermal differentiation and is involved in the development of various 
      cancers. METHODS: qPCR was used to detect the expression level of TINCR in 
      tissues and cell lines of laryngeal squamous cell carcinoma (LSCC). The potential 
      targets of TINCR were predicted by the bioinformation website. The expression of 
      miR-210 and BTG2 genes were detected by qPCR, and the protein levels of BTG2 and 
      Ki-67 were evaluated by western blot. CCK-8 assay, scratch test, and transwell 
      chamber were used to evaluate the proliferation, invasion, and metastasis ability 
      of LSCC cells. The relationships among TINCR, miR-210, and BTG2 were investigated 
      by bioinformatics software and luciferase reporter assay. The in vivo function of 
      TINCR was accessed on survival rate and tumor growth in nude mice. RESULTS: We 
      used qRT-PCR to detect the expression of TINCR in laryngeal squamous cell 
      carcinoma (LSCC) tissues and cells and found significantly lower levels in cancer 
      tissues compared with adjacent tissues. Additionally, patients with high TINCR 
      expression had a better prognosis. TINCR overexpression was observed to inhibit 
      the proliferation and invasion of LSCC cells. TINCR was shown to exert its 
      antiproliferation and invasion effects by adsorbing miR-210, which significantly 
      promoted the proliferation and invasion of laryngeal squamous cells. 
      Overexpression of miR-210 was determined to reverse the tumour-suppressive 
      effects of TINCR. BTG2 (anti-proliferation factor 2) was identified as the target 
      gene of miR-210, and BTG2 overexpression inhibited the proliferation and invasion 
      of LSCC cells. BTG2 knockdown relieved the inhibitory effects of TINCR on the 
      proliferation and invasion of LSCC. Finally, TINCR upregulation slowed xenograft 
      tumour growth in nude mice and significantly increased survival compared with 
      control mice. CONCLUSION: The results of this study suggest that TINCR inhibits 
      the proliferation and invasion of LSCC by regulating the miR-210/BTG2 pathway, 
      participates in cell cycle regulation, and may become a target for the treatment 
      of LSCC.
FAU - He, Guoqing
AU  - He G
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246 Xuefu Road, Harbin, 150086, China.
FAU - Pang, Rui
AU  - Pang R
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Han, Jihua
AU  - Han J
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Jia, Jinliang
AU  - Jia J
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Ding, Zhaoming
AU  - Ding Z
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Bi, Wen
AU  - Bi W
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Yu, Jiawei
AU  - Yu J
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Chen, Lili
AU  - Chen L
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China.
FAU - Zhang, Jiewu
AU  - Zhang J
AD  - Department of Head and Neck Surgery, Harbin Medical University Cancer Hospital, 
      No.150 Haping Road, Harbin, 150081, China. Zhang_Jwu_nic@126.com.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 246 Xuefu Road, Harbin, 150086, China. 
      syn2767@126.com.
LA  - eng
GR  - 81572472, 81272965, 81241085, 81372902, 81402257 and 81402234/the National 
      Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210629
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - 0 (MIRN210 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TINCR lncRNA, human)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Humans
MH  - Laryngeal Neoplasms/pathology
MH  - Mice
MH  - Mice, Nude
MH  - MicroRNAs/*metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - Transfection
PMC - PMC8243464
OTO - NOTNLM
OT  - BTG2
OT  - Laryngeal squamous cell carcinoma
OT  - TINCR
OT  - TU212
OT  - miR-210
COIS- The authors declare that they have no competing interests.
EDAT- 2021/07/01 06:00
MHDA- 2021/10/21 06:00
PMCR- 2021/06/29
CRDT- 2021/06/30 05:35
PHST- 2020/09/27 00:00 [received]
PHST- 2021/06/15 00:00 [accepted]
PHST- 2021/06/30 05:35 [entrez]
PHST- 2021/07/01 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/06/29 00:00 [pmc-release]
AID - 10.1186/s12885-021-08513-0 [pii]
AID - 8513 [pii]
AID - 10.1186/s12885-021-08513-0 [doi]
PST - epublish
SO  - BMC Cancer. 2021 Jun 29;21(1):753. doi: 10.1186/s12885-021-08513-0.