PMID- 34193399
OWN - NLM
STAT- MEDLINE
DCOM- 20211028
LR  - 20211028
IS  - 1664-2848 (Electronic)
IS  - 0036-7281 (Linking)
VI  - 163
IP  - 7
DP  - 2021 Jul
TI  - [Detection of porcine reproductive and respiratory syndrome virus (PRRS) genome 
      fragments and anti-- bodies in chewing ropes allow -monitoring of PRRS in pig 
      farms].
PG  - 471-484
LID - 10.17236/sat00308 [doi]
AB  - Saliva samples from chewing ropes are a reliable diagnostic of porcine 
      reproductive and respiratory syndrome virus (PRRSV) infections. The aim of this 
      study was to test whether saliva samples taken with saliva swabs (cotton swabs 
      and GenoTube Livestock) or with chewing ropes are suitable for monitoring PRRSV 
      in unsuspicious farms, this means to detect a prevalence of 20% infected animals 
      with a 95% probability. Saliva samples were collected from 12-16 pens in five pig 
      farms by using a chewing rope for collective samples and by individual saliva 
      swaps from five randomly selected animals per pen. A total of 291 animals from 58 
      pens in four study farms and 60 animals from 12 pens in one control farm were 
      collected. The samples were taken from all age categories. According to the 
      current monitoring system the analysis of five individual serum samples from the 
      same pens served as the reference method for the relative sensitivity of the 
      saliva samples. Serum and chewing rope samples were tested by ELISA for 
      antibodies. Two different systems were used for the serum samples. Chewing ropes, 
      saliva swabs (GenoTube Livestock) and serum samples were examined for virus 
      genomes using a nested reverse-transcriptase PCR and a commercial real-time 
      reverse-transcriptase PCR kit. Cohen's Kappa was used as a measure of agreement. 
      PRRSV antibodies were detected in the chewing ropes of 44 pens and in the serum 
      samples of only 34 pens. Viral RNA was found in 13 (chewing ropes), respectively 
      16 pens (serum samples). Saliva swabs (GenoTube Livestock) showed a lower 
      relative sensitivity of 20.00% compared to serum samples. The agreement of the 
      two serum analysis was very good for the ELISAs (kappa = 0,911), and moderate for the 
      PCR (kappa = 0,706). The comparison of the chewing rope method with the analysis of 
      the serum samples advocates this method as a suitable supplementary monitoring 
      tool in PRRSV unsuspicious pig farms. Easy handling and lower examination costs 
      of the chewing rope method allow higher testing frequency and would therefore 
      improve the monitoring system. However, they are not an alternative to serum 
      samples. Sampling with saliva swabs is unsuitable.
FAU - David, K
AU  - David K
AD  - Thuringer Tierseuchenkasse AdoR, Tiergesundheitsdienst, Jena, Deutschland.
FAU - Dittmar, K
AU  - Dittmar K
AD  - Thuringer Tierseuchenkasse AdoR, Tiergesundheitsdienst, Jena, Deutschland.
FAU - Heenemann, K
AU  - Heenemann K
AD  - Universitat Leipzig, Veterinarmedizinische Fakultat, Zentrum fur 
      Infektionsmedizin, Institut fur Virologie, Deutschland.
FAU - Reiner, G
AU  - Reiner G
AD  - Justus-Liebig-Universitat Giessen, Klinik fur Schweine (Innere Medizin und 
      Chirurgie), Deutschland.
FAU - Donat, K
AU  - Donat K
AD  - Thuringer Tierseuchenkasse AdoR, Tiergesundheitsdienst, Jena, Deutschland.
AD  - Klinik fur Geburtshilfe, Gynakologie und Andrologie der Gross- und Kleintiere mit 
      Tierarztlicher Ambulanz der Justus-Liebig-Universitat Giessen.
LA  - ger
PT  - Journal Article
TT  - Der Nachweis von Genomfragmenten des Porcinen Reproduktiven und -Respiratorischen 
      Syndrom Virus (PRRSV) und -spezifischen Antikorpern in Kaustricken ermoglicht die 
      Uberwachung PRRSV-unverdachtiger schweine-haltender Betriebe.
PL  - Switzerland
TA  - Schweiz Arch Tierheilkd
JT  - Schweizer Archiv fur Tierheilkunde
JID - 0424247
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Farms
MH  - Mastication
MH  - *Porcine Reproductive and Respiratory Syndrome/diagnosis
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - *Swine Diseases
OTO - NOTNLM
OT  - Coefficient Kappa de Cohen
OT  - Cohen's Kappa coefficient
OT  - Cohen's Kappa-Koeffizient
OT  - ELISA
OT  - RT-PCR en temps reel
OT  - RT-PCR in tempo reale
OT  - Schweine
OT  - Sensitivitat
OT  - Speichelproben
OT  - campioni di saliva
OT  - coefficiente Kappa di Cohen
OT  - pigs
OT  - porcs
OT  - real-time -RT-PCR
OT  - saliva samples
OT  - sensibilita
OT  - sensibilite
OT  - sensitivity
OT  - suini
OT  - -real-time RT-PCR
OT  - echantillons de salive
EDAT- 2021/07/02 06:00
MHDA- 2021/10/29 06:00
CRDT- 2021/07/01 06:12
PHST- 2021/07/01 06:12 [entrez]
PHST- 2021/07/02 06:00 [pubmed]
PHST- 2021/10/29 06:00 [medline]
AID - 10.17236/sat00308 [doi]
PST - ppublish
SO  - Schweiz Arch Tierheilkd. 2021 Jul;163(7):471-484. doi: 10.17236/sat00308.