PMID- 34195070
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - Long Noncoding RNA SNHG12 Promotes Gastric Cancer Proliferation by Binding to HuR 
      and Stabilizing YWHAZ Expression Through the AKT/GSK-3beta Pathway.
PG  - 645832
LID - 10.3389/fonc.2021.645832 [doi]
LID - 645832
AB  - BACKGROUND: Gastric cancer (GC) is a malignancy with high morbidity and mortality 
      rates worldwide. SNHG12 is a long noncoding RNA (lncRNA) commonly involved many 
      types of cancers in the contexts of tumorigenesis, migration and drug resistance. 
      Nevertheless, its role in GC proliferation is poorly understood. METHODS: 
      Bioinformatics and qRT-PCR assays were used to analyze the expression of SNHG12 
      in GC tissues and cells. In vitro and in vivo experiments were conducted to 
      detect the role of SNHG12 in GC development. qRT-PCR, PCR, western blotting (WB), 
      RNA binding protein immunoprecipitation (RIP), immunoprecipitation (IP), 
      immunohistochemistry (IHC), fluorescence in situ hybridization (FISH) and in situ 
      hybridization (ISH) were performed to investigate the underlying mechanisms by 
      which SNHG12 promotes GC proliferation. RESULTS: SNHG12 was highly expressed in 
      GC cells and tissues, and predicted poor survival. In vitro and in vivo assays 
      showed that SNHG12 knockdown inhibited GC proliferation, while SNHG12 
      overexpression promoted GC proliferation. Further experiments confirmed that 
      SNHG12 was mainly located in the cytoplasm and bound to HuR. Bioinformatics 
      analysis predicted that YWHAZ was the common target of SNHG12 and HuR, and that 
      the "SNHG12-HuR" complex enhanced the stability of YWHAZ mRNA. Furthermore, 
      YWHAZ, which was highly expressed in GC, predicted poor survival and promoted GC 
      proliferation by phosphorylating AKT. Rescue assays verified that SNHG12 promoted 
      GC proliferation by activating the AKT/GSK-3beta pathway. CONCLUSIONS: SNHG12 binds 
      to HuR and stabilizes YWHAZ. SNHG12 promotes GC proliferation via modulation of 
      the YWHAZ/AKT/GSK-3beta axis in vitro and in vivo. Thus, SNHG12 could become a novel 
      therapeutic target for anti-tumor therapy.
CI  - Copyright (c) 2021 Zhang, Beeharry, Zheng, Wang, Li, Zhu and Li.
FAU - Zhang, Tianqi
AU  - Zhang T
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Beeharry, Maneesh Kumarsing
AU  - Beeharry MK
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Zheng, Yanan
AU  - Zheng Y
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Wang, Zhenqiang
AU  - Wang Z
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Li, Jianfang
AU  - Li J
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Zhu, Zhenggang
AU  - Zhu Z
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Li, Chen
AU  - Li C
AD  - Department of General Surgery, Shanghai Key Laboratory of Gastric Neoplasms, 
      Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210614
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8236831
OTO - NOTNLM
OT  - HuR
OT  - SNHG12
OT  - YWHAZ
OT  - gastric cancer
OT  - proliferation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/02 06:00
MHDA- 2021/07/02 06:01
PMCR- 2021/01/01
CRDT- 2021/07/01 07:02
PHST- 2020/12/24 00:00 [received]
PHST- 2021/05/17 00:00 [accepted]
PHST- 2021/07/01 07:02 [entrez]
PHST- 2021/07/02 06:00 [pubmed]
PHST- 2021/07/02 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.645832 [doi]
PST - epublish
SO  - Front Oncol. 2021 Jun 14;11:645832. doi: 10.3389/fonc.2021.645832. eCollection 
      2021.