PMID- 34198263
OWN - NLM
STAT- MEDLINE
DCOM- 20210729
LR  - 20210729
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 13
DP  - 2021 Jul 1
TI  - Plasma cell-free circRNAs panel act as fingerprint predicts the occurrence of 
      laryngeal squamous cell carcinoma.
PG  - 17328-17336
LID - 10.18632/aging.203215 [doi]
AB  - BACKGROUND: Circular RNAs (circRNAs) have recently emerged as a new class of 
      RNAs, highly enriched in the human tissues and very stable within cells, exosomes 
      and body fluids. In this study, we aimed to screen the plasma cell-free derived 
      circRNAs in laryngeal squamous cell carcinoma (LSCC) and investigate whether 
      these circRNAs could predicted LSCC as potential biomarkers. METHODS: The circRNA 
      microarray was employed with three samples in each group to screen the 
      dysregulated circRNAs isolated from plasma samples. The top 20 circRNAs were 
      first selected as candidates with the upregulated level in the plasma of LSCC. 
      RESULTS: Further validation found that only circ_0019201, circ_0011773 and 
      circ_0122790 was consistent with training set. The ROC curve also revealed a high 
      diagnostic ability an area under ROC curve value (AUC) for single circRNA and 
      combined. The AUC for circ_0019201, circ_0011773 and circ_0122790 and the 
      combined was 0.933, 0.908, 0.965 and 0.990 in training set. For the validation 
      set, the AUC was 0.766, 0.864, 0.908 and 0.951. The three circRNAs were further 
      investigated with stable expression in human plasma samples. CONCLUSIONS: The 
      plasma derived circ_0019201, circ_0011773 and circ_0122790 might be the potential 
      biomarker for predicting the LSCC.
FAU - Han, Jiahui
AU  - Han J
AD  - Department of Otolaryngology Head and Neck Surgery, The First People's Hospital 
      of Lianyungang, The Affiliated Lianyungang Hospital of Xuzhou Medical University, 
      Lianyungang, Jiangsu Province 222061, PR China.
FAU - Lin, Qiuhong
AU  - Lin Q
AD  - Department of Otolaryngology Head and Neck Surgery, The First People's Hospital 
      of Lianyungang, The Affiliated Lianyungang Hospital of Xuzhou Medical University, 
      Lianyungang, Jiangsu Province 222061, PR China.
FAU - Dong, Chunguang
AU  - Dong C
AD  - Department of Otolaryngology Head and Neck Surgery, The First People's Hospital 
      of Lianyungang, The Affiliated Lianyungang Hospital of Xuzhou Medical University, 
      Lianyungang, Jiangsu Province 222061, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210701
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell-Free Nucleic Acids)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Case-Control Studies
MH  - Cell-Free Nucleic Acids
MH  - DNA Fingerprinting/*methods
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Microarray Analysis
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Predictive Value of Tests
MH  - RNA, Circular/*genetics
MH  - ROC Curve
MH  - Reproducibility of Results
MH  - Retrospective Studies
PMC - PMC8312444
OTO - NOTNLM
OT  - circRNA
OT  - laryngeal squamous cell carcinoma
OT  - plasma
OT  - receiver operating characteristic curve
OT  - risk score function
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2021/07/02 06:00
MHDA- 2021/07/30 06:00
PMCR- 2021/07/15
CRDT- 2021/07/01 20:28
PHST- 2021/05/21 00:00 [received]
PHST- 2021/06/08 00:00 [accepted]
PHST- 2021/07/02 06:00 [pubmed]
PHST- 2021/07/30 06:00 [medline]
PHST- 2021/07/01 20:28 [entrez]
PHST- 2021/07/15 00:00 [pmc-release]
AID - 203215 [pii]
AID - 10.18632/aging.203215 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2021 Jul 1;13(13):17328-17336. doi: 10.18632/aging.203215. 
      Epub 2021 Jul 1.