PMID- 34198747
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210714
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 10
IP  - 6
DP  - 2021 Jun 5
TI  - Insights into Temperature and Hypoxia Tolerance in Cowpea Weevil via HIF-1.
LID - 10.3390/pathogens10060704 [doi]
LID - 704
AB  - Cowpea weevil (Callosobruchus maculatus) is a major pest that leads to severe 
      damage of the stored leguminous grains. Several management approaches, including 
      physical barriers, biological or chemical methods, are used for controlling 
      bruchid in cowpea. These methods usually target the metabolically active state of 
      weevil. However, it becomes less effective at early stages as egg, larva, or pupa 
      under low temperature and oxygen conditions. Since hypoxia-inducible factor-1 
      (HIF-1) is known to coordinate multiple gene responses to low oxygen or low 
      temperature signals, we examined the HIF-1alpha gene expression under low temperature 
      and hypoxic treatments. At -20  degrees C, it took 4 h to reduce the survival rate for 
      eggs, larvae, and pupae down to 10%, while at 4  degrees C and 15  degrees C, the survival rate 
      remained higher than 50% even after 128 h as HIF-1alpha gene expression peaked at 15 
       degrees C. Moreover, HIF-1 protein offers a valuable target for early stage pest control 
      complementary to traditional methods. In particular, HIF-1 inhibitor camptothecin 
      (CPT), one of the five HIF-1 inhibitors examined, achieved a very significant 
      reduction of 96.2% and 95.5% relative to the control in weevil survival rate into 
      adult at 4  degrees C and 30  degrees C, respectively. Our study can be used as one model system 
      for drug development in virus infections and human cancer.
FAU - Liu, Qin
AU  - Liu Q
AD  - School of Life Sciences, Jianghan University, Wuhan 430056, China.
FAU - Liu, Zhichao
AU  - Liu Z
AD  - School of Biological Information, Chongqing University of Posts and 
      Telecommunications, Chongqing 400065, China.
FAU - Gao, Zhipeng
AU  - Gao Z
AD  - School of Life Sciences, Jianghan University, Wuhan 430056, China.
FAU - Chen, Guanjun
AU  - Chen G
AD  - School of Life Sciences, Jianghan University, Wuhan 430056, China.
FAU - Liu, Changyan
AU  - Liu C
AD  - Institute of Food Crop, Hubei Academy of Agricultural Sciences, Wuhan 430064, 
      China.
FAU - Wan, Zhenghuang
AU  - Wan Z
AD  - Institute of Food Crop, Hubei Academy of Agricultural Sciences, Wuhan 430064, 
      China.
FAU - Chen, Chanyou
AU  - Chen C
AD  - School of Life Sciences, Jianghan University, Wuhan 430056, China.
FAU - Zeng, Chen
AU  - Zeng C
AUID- ORCID: 0000-0001-7778-6388
AD  - Department of Physics, The George Washington University, Washington, DC 20052, 
      USA.
FAU - Zhao, Yunjie
AU  - Zhao Y
AD  - Institute of Biophysics and Department of Physics, Central China Normal 
      University, Wuhan 430079, China.
FAU - Pan, Lei
AU  - Pan L
AD  - School of Life Sciences, Jianghan University, Wuhan 430056, China.
LA  - eng
GR  - 11704140/National Natural Science Foundation of China/
GR  - 201708420039/China Scholarship Council Fund/
GR  - CCNU20TS004/self-determined research funds of CCNU from the colleges' basic 
      research and operation of MOE/
PT  - Journal Article
DEP - 20210605
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC8228136
OTO - NOTNLM
OT  - HIF-1alpha
OT  - cowpea weevil
OT  - inhibitor screening
OT  - mRNA transcript
COIS- All authors declare no conflict of interest in this paper.
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
PMCR- 2021/06/05
CRDT- 2021/07/02 01:01
PHST- 2021/04/25 00:00 [received]
PHST- 2021/05/29 00:00 [revised]
PHST- 2021/06/01 00:00 [accepted]
PHST- 2021/07/02 01:01 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
PHST- 2021/06/05 00:00 [pmc-release]
AID - pathogens10060704 [pii]
AID - pathogens-10-00704 [pii]
AID - 10.3390/pathogens10060704 [doi]
PST - epublish
SO  - Pathogens. 2021 Jun 5;10(6):704. doi: 10.3390/pathogens10060704.