PMID- 34199095
OWN - NLM
STAT- MEDLINE
DCOM- 20210816
LR  - 20210816
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 13
IP  - 7
DP  - 2021 Jun 27
TI  - Myoinositol Reduces Inflammation and Oxidative Stress in Human Endothelial Cells 
      Exposed In Vivo to Chronic Hyperglycemia.
LID - 10.3390/nu13072210 [doi]
LID - 2210
AB  - Myo-inositol (Myo) improves insulin resistance, glucose metabolism, and helps 
      gestational diabetes (GDM) management. GDM is associated with a pro-inflammatory 
      state and increased oxidative stress, which are both involved in vascular damage 
      in diabetes. Our aim was to study Myo anti-inflammatory/antioxidant potential 
      effects on an in vitro model of human umbilical vein endothelial cells (HUVECs). 
      To this end, monocyte cell adhesion to HUVECs, adhesion molecule membrane 
      exposure, and oxidative stress levels were determined in cells from control (C-) 
      and GDM women treated during pregnancy either with diet only (GD-) or with diet 
      plus Myo (GD+Myo). To deeply study the vascular effects of Myo, the same 
      evaluations were performed in C- and GD-HUVECs following 48 h in vitro 
      stimulation with Myo. Notably, we first observed that GD-HUVECs obtained from 
      women assuming Myo supplementation exhibited a significantly decreased number of 
      monocytes that adhered to endothelial cells, less adhesion molecule exposure, and 
      lower intracellular reactive oxygen species (ROS) levels in the basal state as 
      compared to GD-HUVECs obtained from women treated by diet only. This Myo 
      anti-inflammatory/antioxidant effect was confirmed by 48 h in vitro stimulation 
      of GD-HUVECs as compared to controls. Altogether, these results strongly suggest 
      that Myo may exert protective actions against chronic inflammation induced by 
      endothelial dysfunction in diabetes.
FAU - Baldassarre, Maria Pompea Antonia
AU  - Baldassarre MPA
AUID- ORCID: 0000-0002-9707-7439
AD  - Department of Medicine and Aging Sciences, G. d'Annunzio University 
      Chieti-Pescara, 66100 Chieti, Italy.
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Di Tomo, Pamela
AU  - Di Tomo P
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
AD  - Department of Medical, Oral and Biotechnological Sciences, G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Centorame, Giorgia
AU  - Centorame G
AD  - Department of Medicine and Aging Sciences, G. d'Annunzio University 
      Chieti-Pescara, 66100 Chieti, Italy.
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Pandolfi, Assunta
AU  - Pandolfi A
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
AD  - Department of Medical, Oral and Biotechnological Sciences, G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Di Pietro, Natalia
AU  - Di Pietro N
AUID- ORCID: 0000-0001-9720-2116
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
AD  - Department of Medical, Oral and Biotechnological Sciences, G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Consoli, Agostino
AU  - Consoli A
AD  - Department of Medicine and Aging Sciences, G. d'Annunzio University 
      Chieti-Pescara, 66100 Chieti, Italy.
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
FAU - Formoso, Gloria
AU  - Formoso G
AD  - Department of Medicine and Aging Sciences, G. d'Annunzio University 
      Chieti-Pescara, 66100 Chieti, Italy.
AD  - Center for Advanced Studies and Technology-CAST (ex CeSI Met), G. d'Annunzio 
      University Chieti-Pescara, 66100 Chieti, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210627
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antioxidants)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Reactive Oxygen Species)
RN  - 4L6452S749 (Inositol)
SB  - IM
MH  - Adult
MH  - Antioxidants/pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/metabolism
MH  - Diabetes, Gestational/metabolism
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/drug effects
MH  - Humans
MH  - Hyperglycemia/*metabolism
MH  - Inflammation/*drug therapy
MH  - Inositol/*therapeutic use
MH  - Monocytes/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Pregnancy
MH  - Reactive Oxygen Species/metabolism
MH  - U937 Cells
PMC - PMC8308270
OTO - NOTNLM
OT  - HUVEC
OT  - endothelial dysfunction
OT  - gestational diabetes
OT  - inositol
OT  - myoinositol
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/03 06:00
MHDA- 2021/08/17 06:00
PMCR- 2021/06/27
CRDT- 2021/07/02 01:02
PHST- 2021/05/27 00:00 [received]
PHST- 2021/06/21 00:00 [revised]
PHST- 2021/06/24 00:00 [accepted]
PHST- 2021/07/02 01:02 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/08/17 06:00 [medline]
PHST- 2021/06/27 00:00 [pmc-release]
AID - nu13072210 [pii]
AID - nutrients-13-02210 [pii]
AID - 10.3390/nu13072210 [doi]
PST - epublish
SO  - Nutrients. 2021 Jun 27;13(7):2210. doi: 10.3390/nu13072210.