PMID- 34204558
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20210702
LR  - 20240402
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 26
IP  - 12
DP  - 2021 Jun 17
TI  - Efficient Generation of Microdroplets Using Tail Breakup Induced with 
      Multi-Branch Channels.
LID - 10.3390/molecules26123707 [doi]
LID - 3707
AB  - In recent years, research on the application of microdroplets in the fields of 
      biotechnology and chemistry has made remarkable progress, but the technology for 
      the stable generation of single-micrometer-scale microdroplets has not yet been 
      established. In this paper, we developed an efficient and stable 
      single-micrometer-scale droplet generation device based on the fragmentation of 
      droplet tails, called "tail thread mode", that appears under moderate flow 
      conditions. This method can efficiently encapsulate microbeads that mimic cells 
      and chemical products in passively generated single-micrometer-scale 
      microdroplets. The device has a simple 2D structure; a T-junction is used for 
      droplet generation; and in the downstream, multi-branch channels are designed for 
      droplet deformation into the tail. Several 1-2 microm droplets were successfully 
      produced by the tail's fragmentation; this continuous splitting was induced by 
      the branch channels. We examined a wide range of experimental conditions and 
      found the optimal flow rate condition can be reduced to one-tenth compared to the 
      conventional tip-streaming method. A mold was fabricated by simple soft 
      lithography, and a polydimethylsiloxane (PDMS) device was fabricated using the 
      mold. Based on the 15 patterns of experimental conditions and the results, the 
      key factors for the generation of microdroplets in this device were examined. In 
      the most efficient condition, 61.1% of the total droplets generated were smaller 
      than 2 mum.
FAU - Tanaka, Daiki
AU  - Tanaka D
AUID- ORCID: 0000-0003-1900-4848
AD  - Research Organization for Nano & Life Innovation, Waseda University, 513 
      Wasedatsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
FAU - Kajiya, Satsuki
AU  - Kajiya S
AD  - Department of Electronic and Physical Systems, School of Fundamental Science and 
      Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 145-0065, Japan.
FAU - Shijo, Seito
AU  - Shijo S
AD  - Department of Electronic and Physical Systems, School of Fundamental Science and 
      Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 145-0065, Japan.
FAU - Yoon, Dong Hyun
AU  - Yoon DH
AUID- ORCID: 0000-0001-7180-4370
AD  - Research Organization for Nano & Life Innovation, Waseda University, 513 
      Wasedatsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
FAU - Furuya, Masahiro
AU  - Furuya M
AUID- ORCID: 0000-0002-1221-2874
AD  - Department of Electronic and Physical Systems, School of Fundamental Science and 
      Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 145-0065, Japan.
FAU - Nozaki, Yoshito
AU  - Nozaki Y
AD  - Research Organization for Nano & Life Innovation, Waseda University, 513 
      Wasedatsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
FAU - Fujita, Hiroyuki
AU  - Fujita H
AD  - Canon Medical Systems Corporation, 1385 Shimoishigami, Otawara-shi, Tochigi 
      324-8550, Japan.
FAU - Sekiguchi, Tetsushi
AU  - Sekiguchi T
AD  - Research Organization for Nano & Life Innovation, Waseda University, 513 
      Wasedatsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
FAU - Shoji, Shuichi
AU  - Shoji S
AD  - Department of Electronic and Physical Systems, School of Fundamental Science and 
      Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 145-0065, Japan.
LA  - eng
GR  - 20H00336/Basic Scientific Research (A) from the Japanese Ministry of Education, 
      Culture, Sports, Science and Technology/
PT  - Journal Article
DEP - 20210617
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
SB  - IM
PMC - PMC8235478
OTO - NOTNLM
OT  - droplet generation
OT  - encapsulation
OT  - microfluidics
OT  - single-micrometer scale
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
PMCR- 2021/06/17
CRDT- 2021/07/02 01:21
PHST- 2021/04/29 00:00 [received]
PHST- 2021/06/10 00:00 [revised]
PHST- 2021/06/14 00:00 [accepted]
PHST- 2021/07/02 01:21 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
PHST- 2021/06/17 00:00 [pmc-release]
AID - molecules26123707 [pii]
AID - molecules-26-03707 [pii]
AID - 10.3390/molecules26123707 [doi]
PST - epublish
SO  - Molecules. 2021 Jun 17;26(12):3707. doi: 10.3390/molecules26123707.