PMID- 34206894
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210727
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 13
IP  - 7
DP  - 2021 Jun 26
TI  - Light- and Melanin Nanoparticle-Induced Cytotoxicity in Metastatic Cancer Cells.
LID - 10.3390/pharmaceutics13070965 [doi]
LID - 965
AB  - Melanin nanoparticles are known to be biologically benign to human cells for a 
      wide range of concentrations in a high glucose culture nutrition. Here, we show 
      cytotoxic behavior at high nanoparticle and low glucose concentrations, as well 
      as at low nanoparticle concentration under exposure to (nonionizing) visible 
      radiation. To study these effects in detail, we developed highly monodispersed 
      melanin nanoparticles (both uncoated and glucose-coated). In order to study the 
      effect of significant cellular uptake of these nanoparticles, we employed three 
      cancer cell lines: VM-M3, A375 (derived from melanoma), and HeLa, all known to 
      exhibit strong macrophagic character, i.e., strong nanoparticle uptake through 
      phagocytic ingestion. Our main observations are: (i) metastatic VM-M3 cancer 
      cells massively ingest melanin nanoparticles (mNPs); (ii) the observed ingestion 
      is enhanced by coating mNPs with glucose; (iii) after a certain level of mNP 
      ingestion, the metastatic cancer cells studied here are observed to die-glucose 
      coating appears to slow that process; (iv) cells that accumulate mNPs are much 
      more susceptible to killing by laser illumination than cells that do not 
      accumulate mNPs; and (v) non-metastatic VM-NM1 cancer cells also studied in this 
      work do not ingest the mNPs, and remain unaffected after receiving identical 
      optical energy levels and doses. Results of this study could lead to the 
      development of a therapy for control of metastatic stages of cancer.
FAU - Gabriele, Victoria R
AU  - Gabriele VR
AD  - Department of Physics, Boston College, Chestnut Hill, MA 02467, USA.
FAU - Mazhabi, Robabeh M
AU  - Mazhabi RM
AD  - International Academy of Optoelectronics at Zhaoqing, South China Normal 
      University, Zhaoqing 526238, China.
FAU - Alexander, Natalie
AU  - Alexander N
AUID- ORCID: 0000-0001-7427-2672
AD  - Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
FAU - Mukherjee, Purna
AU  - Mukherjee P
AD  - Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
FAU - Seyfried, Thomas N
AU  - Seyfried TN
AUID- ORCID: 0000-0003-1491-3989
AD  - Department of Biology, Boston College, Chestnut Hill, MA 02467, USA.
FAU - Nwaji, Njemuwa
AU  - Nwaji N
AUID- ORCID: 0000-0003-2350-1396
AD  - International Academy of Optoelectronics at Zhaoqing, South China Normal 
      University, Zhaoqing 526238, China.
FAU - Akinoglu, Eser M
AU  - Akinoglu EM
AD  - International Academy of Optoelectronics at Zhaoqing, South China Normal 
      University, Zhaoqing 526238, China.
FAU - Mackiewicz, Andrzej
AU  - Mackiewicz A
AD  - Greater Poland Cancer Centre, Poznan University of Medical Sciences, 61-867 
      Poznan, Poland.
FAU - Zhou, Guofu
AU  - Zhou G
AUID- ORCID: 0000-0002-4104-403X
AD  - International Academy of Optoelectronics at Zhaoqing, South China Normal 
      University, Zhaoqing 526238, China.
AD  - Guangdong Provincial Key Laboratory of Optical Information Materials and 
      Technology & Institute of Electronic Paper Displays, South China Academy of 
      Advanced Optoelectronics, South China Normal University, Guangzhou 510006, China.
FAU - Giersig, Michael
AU  - Giersig M
AUID- ORCID: 0000-0002-5394-0413
AD  - International Academy of Optoelectronics at Zhaoqing, South China Normal 
      University, Zhaoqing 526238, China.
AD  - Institute of Fundamental Technological Research of Polish Academy of Sciences, 
      02-106 Warsaw, Poland.
FAU - Naughton, Michael J
AU  - Naughton MJ
AUID- ORCID: 0000-0002-6733-2398
AD  - Department of Physics, Boston College, Chestnut Hill, MA 02467, USA.
FAU - Kempa, Krzysztof
AU  - Kempa K
AD  - Department of Physics, Boston College, Chestnut Hill, MA 02467, USA.
LA  - eng
GR  - 1748906/National Science Foundation/
GR  - 2016ZT06C517/Guangdong Innovative and Entrepreneurial Research Team Program/
PT  - Journal Article
DEP - 20210626
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC8309021
OTO - NOTNLM
OT  - cytotoxicity
OT  - hyperthermia
OT  - laser medical applications
OT  - melanin nanoparticles
OT  - melanoma
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/03 06:00
MHDA- 2021/07/03 06:01
PMCR- 2021/06/26
CRDT- 2021/07/02 01:29
PHST- 2021/05/21 00:00 [received]
PHST- 2021/06/13 00:00 [revised]
PHST- 2021/06/23 00:00 [accepted]
PHST- 2021/07/02 01:29 [entrez]
PHST- 2021/07/03 06:00 [pubmed]
PHST- 2021/07/03 06:01 [medline]
PHST- 2021/06/26 00:00 [pmc-release]
AID - pharmaceutics13070965 [pii]
AID - pharmaceutics-13-00965 [pii]
AID - 10.3390/pharmaceutics13070965 [doi]
PST - epublish
SO  - Pharmaceutics. 2021 Jun 26;13(7):965. doi: 10.3390/pharmaceutics13070965.